3DS MAX三维动画在胶体电泳MCAI课件中的应用

介绍了用3DS MAX动画技术制作模拟胶体电泳实验MCAI课件的过程。通过动画演示，形象、生动、直观地表达了胶体形成过程中溶液颜色的逐渐变化以及电泳过程中溶胶和介质之间的界面的移动。利用此课件取代课堂教学的演示实验，可以改进教学效果。     

脉冲电磁场对雄性小白鼠生物效应的研究

本文分析了生物体所处电磁环境的类型以及外电磁场对生物体的作用方式，并以雄性小白鼠为研究对象.采用血细胞计数及细胞电泳实验的方法研究了脉冲电场及脉冲磁场对其影响。在生物电介质介电模型的基础上，探讨了脉冲电磁场对生物体及其细胞作用的机理。     

毛细管区带电泳的紫外可见光吸收检测

利用紫外可见光吸收方法完成毛细管区带电泳的检测工作，本文就实验装置，实验结果进行了讨论。     

汽车电泳涂装工艺

在通常情况下,经过表面处理的车身要经过底漆、中涂和面漆3个涂装过程.从阴极电泳漆的性能入手,谈车身底漆的电泳涂装的特点、工艺和检测方法,旨在介绍电泳涂装的方法原理和工艺技巧.     

基于荧光显微镜的毛细管电泳系统的构建

毛细管电泳仪具有灵敏度高、分析速度快等优势,为降低其生产成本,基于电泳原理,以荧光显微镜为基础,设计了一套毛细管电泳系统。以20 bp(base pairs,碱基对)DNA ladder和100 bp DNA ladder为样本,全面分析了系统的稳定性、灵敏度和分离效果。结果表明:该系统在9 min内可以实现1500 bp以内DNA片段的高效分离,系统检测极限为0.1 ng/μL;在优化的电泳条件下,对限制性内切酶φX174-HincⅡ作用过的λ-DNA片段5 min内实现了291 bp与297 bp DNA片段的区分。     

苏州市食品中单增李斯特菌污染状况及分子特征分析

目的 研究2016—2020年苏州市各类食品中单增李斯特菌的污染状况及分子特征。方法 将采样食品按WS/T464—2015《食物成分数据表达规范》进行分类,计算各类食品污染率,通过聚合酶链式反应(polymerase chain reaction, PCR)和凝胶电泳对毒力基因(inlA、inlB)和耐药相关基因(virR、dltA、dltB、dltC、dltD、mprF)进行检测,通过脉冲场凝胶电泳进行分子分型。结果 683件食品中共检出44件阳性样品,总体检出率6.4%。各类食品中以肉类食品的检出率最高(17.2%),肉类食品中以预制肉制品检出率最高(37.2%)。分离的44株单增李斯特菌毒力基因inlA携带率为100.0%,inlB携带率为97.7%,耐药相关基因virR、dltA、dltB、dltC、dltD、mprF的携带率分别为90.9%、100.0%、90.9%、100.0%、95.5%、97.7%。分离株的PFGE型别可分为11个基因型簇,相似度为53.3%～100.0%,有11组100.0%相似度的带型,其中有3组为同一时间,同一地点采集样品所分离菌株。结论 苏州市肉...     

影响低电压电泳芯片分离的主要因素

运用简化的低电压电泳芯片的运动梯度场的分离和控制模型 ,对低电压芯片的各参数与分离度、分离效率的关系进行了讨论     

筒状复杂组合件阴极电泳涂装质量问题的解决办法

分析了防护装备生产过程中筒状复杂组合件阴极电泳涂装时出现阴阳面、表面色彩不均匀、异常附着、缩孔、水滴迹、二次流痕等缺陷的原因,提出了相应的解决办法。     

Occurrence and traceability of Listeria monocytogenes strains isolated from sheep's milk cheese-making plants environment

The aim of the study was to conduct an extensive survey on Listeria monocytogenes and Listeria spp. environmental contamination in 13 cheese-making plants. A total of 409 environmental and food samples were collected during years 2011–2013. Listeria spp. contamination was observed in all the facilities, while L. monocytogenes was recovered from 12 facilities with a prevalence ranging between 3.0% and 22.6%. Floor drains were the most contaminated sampling sites (48.8% of positive samples), serving as harbourage site for subsequent contamination. Out of 616 isolates, 277 (45.0%) were Listeria innocua, 274 (44.5%) L. monocytogenes, 41 (6.6%) Listeria ivanovii, 14 (2.3%) Listeria welshimeri and 10 (1.6%) Listeria gravyi. Serotyping carried out by PCR and agglutination method for L. monocytogenes revealed that 169 strains (61.7%) were serotype 1/2a, 65 (23.7%) 4b, 20 (7.3%) 1/2b, 10 (3.6%) 3a, 7 (2.5%) 1/2c and 3 (1.1%) 3b. PFGE conducted on L. monocytogenes isolates using AscI and ApaI restriction enzymes, yielded 6 clusters. Two predominant PFGE clusters were observed including respectively 36 and 32 strains. Within cheese-making plants, L. monocytogenes showed wide variability with strains distributed up to 4 different clusters. Pulsotypes isolated from raw milk filter were never detected in the processing environment, indicating that the contamination originated from sources other than raw milk. The isolation of strains with similar profile from different sampling sites, within and among cheese-making plants, indicated the possible transfer of L. monocytogenes contamination along production lines and from one facility to another. Strains recovered from food were confirmed as originating from the processing environment.     

Dendrimer as nanocarrier for drug delivery

Dendrimers are novel three dimensional, hyperbranched globular nanopolymeric architectures. Attractive features like nanoscopic size, narrow polydispersity index, excellent control over molecular structure, availability of multiple functional groups at the periphery and cavities in the interior distinguish them amongst the available polymers. Applications of dendrimers in a large variety of fields have been explored. Drug delivery scientists are especially enthusiastic about possible utility of dendrimers as drug delivery tool. Terminal functionalities provide a platform for conjugation of the drug and targeting moieties. In addition, these peripheral functional groups can be employed to tailor-make the properties of dendrimers, enhancing their versatility. The present review highlights the contribution of dendrimers in the field of nanotechnology with intent to aid the researchers in exploring dendrimers in the field of drug delivery.