阴极电泳涂装中MEQ值测定的探讨

介绍了阴极电泳涂装中MEQ值的作用及概念。研究了不同测定方法及NaOH和KOH-C2H5OH标液对MEQ测量值的影响,以及该值对漆膜外观和漆液稳定性的影响。     

Effect of Substrate Composition (C/N/P ratio) on Microbial Community and Membrane Fouling Tendency of Biomass in Membrane Bioreactors

In this study, biomass characteristics including bacterial community, extracellular polymeric substances (EPS) production, and membrane fouling propensity were examined when the membrane bioreactors (MBRs) were fed with different substrates (i.e., different C/N/P ratios). Denaturing gradient gel electrophoresis (DGGE) analysis revealed that significant shifts of bacterial communities happened when increasing nitrogen or phosphorus loading in the MBRs, which followed in an almost similar way. At steady state, the biomass from the low C/N- and C/P-MBRs had comparable concentrations and produced similar EPS levels as those in the control MBR. However, the median particle size increased when the MBRs fed with low C/N- or C/P- substrate, possibly associated with the filamentous bacteria propagating in the MBRs. Increasing nitrogen or phosphorus loading 1-fold could not induce more serious membrane fouling compared to the control MBR.     

配位作用毛细管电泳分离天冬中氨基酸

基于配位相互作用的毛细管电泳技术,利用Cu2＋与氨基酸的配位作用,形成具有不同离子淌度的络合物,在50 mmol/L的CuSO4缓冲液中可实现中药天冬中氨基酸的有效分离。在最优条件下,被分离的氨基酸分别为：赖氨酸、组氨酸、精氨酸、甘氨酸、色氨酸、亮氨酸、缬氨酸、谷氨酸。该法无需样品衍生处理,快速、简便,灵敏度较高,值得推广。     

筒状复杂组合件阴极电泳涂装质量问题的解决办法

分析了防护装备生产过程中筒状复杂组合件阴极电泳涂装时出现阴阳面、表面色彩不均匀、异常附着、缩孔、水滴迹、二次流痕等缺陷的原因,提出了相应的解决办法。     

The effect of perspiration on photo-induced chemical reaction of azo dyes and the determination of aromatic amine products

In order to investigate the impact of perspiration on photo-induced chemical reaction of azo dyes and the carcinogenic aromatic amine products produced from the reaction, we have carried out experimental studies on the photochemical reaction of C.I. Reactive Red 2 mixed with American Association of Textile Chemists and Colorists（AATCC）standard artificial perspiration. UV-vis spectroscopic technique was employed to monitor the reaction processes, and the reaction products were analyzed by hollow fiber protected liquid-liquid-liquid phase micro-extraction with capillary electrophoresis（HF-LLLME-CE). The results showed that perspiration had remarkable influence on the photochemical reaction of azo dyes. Aromtic amines formed during the photochemical process as a result of reduction of azo dyes by organic components in perspiration. The HF-LLLME-CE methodology was validated in analyzing aromatic amines produced from the photochemical degradation of azo dye C.I. Reactive Red 2 and C.I. Acid Red 35 mixed with artificial perspiration.     

Identification of tillering node proteins differentially accumulated in barley recombinant inbred lines with different juvenile growth habits

Barley (Hordeum vulgare L.) is an important cereal crop grown for both the feed and malting industries. The allelic dwarfing gene sdw1/denso has been used throughout the world to develop commercial barley varieties. Proteomic analysis offers a new approach to identify a broad spectrum of genes that are expressed in the living system. Two-dimensional electrophoresis and mass spectrometry were applied to investigate changes in protein abundance associated with different juvenile growth habit as effect of the denso locus in barley homozygous lines derived from a Maresi × Pomo cross combination. A total of 31 protein spots were revealed that demonstrate quantitative differences in protein abundance between the analyzed plants with different juvenile growth habit, and these protein spots were selected to be identified by mass spectrometry. Identification was successful for 27 spots, and functional annotations of proteins revealed that most of them are involved in metabolism and disease/defense-related processes. Functions of the identified proteins and their probable influence on the growth habit in barley are discussed.     

客车涂装底层材料配套性分析

针对南方客户在使用我司客车一段时间后,车身涂层不同程度出现了起泡的质量问题,进行了现场查看,发现起泡位置在车身左右侧蒙皮,且发生在涂层最底层。初步分析原因是热镀锌板+阴极电泳漆+原子灰三者之间配套存在问题,通过热镀锌板+阴极电泳漆+原子灰三者之间的耐高温80℃配套试验,以及室外曝晒试验,得出镀锌板表面是否钝化、电泳漆膜致密程度和原子灰苯乙烯含量是造成涂层底层起泡的主要原因。为了控制涂层起泡问题,重新修订了我司原子灰、镀锌板、电泳漆的技术标准以及部分工艺文件。     

2—甲基—1，4—萘醌对DNA光敏损伤的诱导作用

选择波长337nm的激光作为激励光源，借助凝胶电泳研究了2－甲基－1，4－萘醌诱导的DNA光敏损伤。结果表明：在氧气饱和、脱氧条件下光敏损伤显著，DNA损伤主要与光子剂量、核酸与萘醌浓度比及DNA存在形式有关。     

两性离子表面活性剂在分析化学中的应用

综述了两性离子表面活性剂在分析检测尤其在色谱分离和毛细管电泳技术中的应用。在色谱分离中,两性表面活性剂首先被用作固定相,能很好地分离海水及雨水中的阴离子;后来随着色谱技术在生物领域的应用,两性表面活性剂又被用作流动相,即把其溶解于电解质溶液中,此流动相能很好地分离含蛋白质的生物样品。两性表面活性剂还被广泛地用于毛细管电泳中,即作毛细管壁上的动态或静态的涂层,此法既可控制电渗流,提高分离效果,又可避免蛋白质等一些样品粘在壁上,是一种较理想的生物分离技术。     

使用毛细管电泳定量分析黑白显影液衰退反应中的对苯二酚和对苯二酚单磺酸盐

使用毛细管电泳分离并定量分析了对苯二酚——— 1种黑白显影液中的显影剂和它的衰退反应产物对苯二酚单磺酸盐。优化了的毛细管电泳分离。