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A sensing head for simultaneous measurement of temperature and strain is presented and analyzed. The proposed configuration is based on the combination of two Bragg gratings, written in different fibres and with different reflectivities, to form a single signature with a reflected step spectrum profile. This characteristic minimizes the spectrum allocated to each sensor in a series multiplexing topology. By measuring the changes in the peak wavelength and spectral width of this signature, resolutions of ±0.65 °C/√Hz and ±2.55 μ/√Hz were achieved for temperature and strain measurements, respectively. 相似文献
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Quantification of Protein‐Induced Membrane Remodeling Kinetics In Vitro with Lipid Multilayer Gratings 下载免费PDF全文
Troy W. Lowry Plengchart Prommapan Aubrey Kusi‐Appiah Nicholas Vafai Ewa A. Bienkiewicz David H. Van Winkle Scott M. Stagg Steven Lenhert 《Small (Weinheim an der Bergstrasse, Germany)》2016,12(4):506-515
The dynamic self‐organization of lipids in biological systems is a highly regulated process that enables the compartmentalization of living systems at micro‐ and nanoscopic scales. Consequently, quantitative methods for assaying the kinetics of supramolecular remodeling such as vesicle formation from planar lipid bilayers or multilayers are needed to understand cellular self‐organization. Here, a new nanotechnology‐based method for quantitative measurements of lipid–protein interactions is presented and its suitability for quantifying the membrane binding, inflation, and budding activity of the membrane‐remodeling protein Sar1 is demonstrated. Lipid multilayer gratings are printed onto surfaces using nanointaglio and exposed to Sar1, resulting in the inflation of lipid multilayers into unilamellar structures, which can be observed in a label‐free manner by monitoring the diffracted light. Local variations in lipid multilayer volume on the surface is used to vary substrate availability in a microarray format. A quantitative model is developed that allows quantification of binding affinity (K D) and kinetics (kon and koff). Importantly, this assay is uniquely capable of quantifying membrane remodeling. Upon Sar1‐induced inflation of single bilayers from surface supported multilayers, the semicylindrical grating lines are observed to remodel into semispherical buds when a critical radius of curvature is reached. 相似文献
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波分复用系统中的交错复用器(Interleaver)技术 总被引:3,自引:1,他引:3
鉴于波分复用技术已成为光纤通信的发展方向,介绍几种新型的交错复用器的原理、结构等情况,对各种器件进行了比较。 相似文献
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