A guided tour into subcellular colocalization analysis in light microscopy

It is generally accepted that the functional compartmentalization of eukaryotic cells is reflected by the differential occurrence of proteins in their compartments. The location and physiological function of a protein are closely related; local information of a protein is thus crucial to understanding its role in biological processes. The visualization of proteins residing on intracellular structures by fluorescence microscopy has become a routine approach in cell biology and is increasingly used to assess their colocalization with well‐characterized markers. However, image‐analysis methods for colocalization studies are a field of contention and enigma. We have therefore undertaken to review the most currently used colocalization analysis methods, introducing the basic optical concepts important for image acquisition and subsequent analysis. We provide a summary of practical tips for image acquisition and treatment that should precede proper colocalization analysis. Furthermore, we discuss the application and feasibility of colocalization tools for various biological colocalization situations and discuss their respective strengths and weaknesses. We have created a novel toolbox for subcellular colocalization analysis under ImageJ, named JACoP, that integrates current global statistic methods and a novel object‐based approach.     

Preserving global features of fluid animation from a single image using video examples

We synthesize animations from a single image by transferring fluid motion of a video example globally.Given a target image of a fluid scene,an alpha matte is required to extract the fluid region.Our me...     

基于Base64编码的数据加密技术

深入分析了Base64编码的原理，给出了基于动态编码表的Base64编码数据加密方法。     

现代产品设计中的区域文化意象研究

区域文化意象的研究,有助于体现产品区域特色和文化内涵.研究了区域文化意象的消费者认知匹配模型,阐述了区域文化意象的消费者认知匹配模型的3个层次.并以良渚文化意象的酒器设计为例进行验证.     

Study on the forecast method for underground coal mine

At present, coal mine fires were forecasted with some temperature, smog, CO, CO2, etc, however, this method can＇t meet the requirements for safe production of coal mines in monitoring accuracy and validity. Overcoming these problems of foregone monitoring methods, using multi-parameters which include fire image, smog, CO, CO2, O2, etc, the paper put forward a synthetical analysis monitor with advanced technology of neural network. The research and application of this method has significance in theory and practical value for coal mine fire forecast.     

基于DCT域的数字水印算法

该文系统介绍了数字水印的原理和应用方法,并研究了一种基于离散余弦变换（DCT）的数字图像水印算法,并借助MATLAB编程工具,实现数字水印的嵌入、提取和攻击测试。     

Efficient visual communication channels

This paper compares the efficiency of uniform quantization in the spatial domain with frequency dependent quantization in the spatial frequency domain, in the context of the end-to-end performance of visual-communication channels. Results show that the minimum data density required for informationally lossless transmission depends on the design of the image-gathering device. The information in the acquired signal, not the energy, dictates the trade-off between data transmission and visual quality. Frequency dependent quantization that maintains the information capacity of the channel while reducing the entropy of the encoded signal, improves its information efficiency. Information bit-allocation is preferable for optimized visual communication for restoration, whereas energy bit-allocation can be used only for image reconstruction.This research was supported by NASA Task Assignment NAS1-18584-90 with the Department of Mathematics, Old Dominion University, Norfolk, Virginia 23529.     

基于神经网络的图像来源识别方法比较研究

随着手机等便携式智能电子设备的普及,图像已成为最重要的信息载体之一,在新闻、社交及司法等领域发挥着重要作用.在享用电子图像带来便捷性的同时,图像处理工具给不法分子通过篡改电子图像实施诈骗等犯罪活动提供了可能,识别图像来源、辨别图像真伪已成为遏制和惩罚此类犯罪活动的重要技术手段.该文讨论了神经网络在图像源识别中的应用方法...     

数字图像三点中值去隔行技术研究

对传统三点中值去隔行算法进行改进,采用同步更新迭代的方法,提高了图像的画面质量.实验证明,该算法不仅降低了时间复杂度,保护图像边缘,而且明显改善了噪声滤波效果.     

Slicing Method for Reverse Engineering Based on Image Mosaic

Reverse engineering dealing with images is traditionally based on image processing and contour recognition. A new method is presented based on the combination of sectional slicing with image mosaic. Sectional contours of the target object are generated by colorful liquid or laser scanning, these images from different views are fused into a set of complete cross-sectional images, thereby the whole practical model is reconstructed in 3D space.