Genetic Dissection of Light-Regulated Adventitious Root Induction in Arabidopsis thaliana Hypocotyls

Photomorphogenic responses of etiolated seedlings include the inhibition of hypocotyl elongation and opening of the apical hook. In addition, dark-grown seedlings respond to light by the formation of adventitious roots (AR) on the hypocotyl. How light signaling controls adventitious rooting is less well understood. Hereto, we analyzed adventitious rooting under different light conditions in wild type and photomorphogenesis mutants in Arabidopsis thaliana. Etiolation was not essential for AR formation but raised the competence to form AR under white and blue light. The blue light receptors CRY1 and PHOT1/PHOT2 are key elements contributing to the induction of AR formation in response to light. Furthermore, etiolation-controlled competence for AR formation depended on the COP9 signalosome, E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC (COP1), the COP1 interacting SUPPRESSOR OF PHYA-105 (SPA) kinase family members (SPA1,2 and 3) and Phytochrome-Interacting Factors (PIF). In contrast, ELONGATED HYPOCOTYL5 (HY5), suppressed AR formation. These findings provide a genetic framework that explains the high and low AR competence of Arabidopsis thaliana hypocotyls that were treated with dark, and light, respectively. We propose that light-induced auxin signal dissipation generates a transient auxin maximum that explains AR induction by a dark to light switch.     

Order vs. Disorder: Cholesterol and Omega-3 Phospholipids Determine Biomembrane Organization

Lipid structural diversity strongly affects biomembrane chemico-physical and structural properties in addition to membrane-associated events. At high concentrations, cholesterol increases membrane order and rigidity, while polyunsaturated lipids are reported to increase disorder and flexibility. How these different tendencies balance in composite bilayers is still controversial. In this study, electron paramagnetic resonance spectroscopy, small angle neutron scattering, and neutron reflectivity were used to investigate the structural properties of cholesterol-containing lipid bilayers in the fluid state with increasing amounts of polyunsaturated omega-3 lipids. Either the hybrid 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine or the symmetric 1,2-docosahexaenoyl-sn-glycero-3-phosphocholine were added to the mixture of the naturally abundant 1-palmitoyl-2-oleyl-sn-glycero-3-phosphocholine and cholesterol. Our results indicate that the hybrid and the symmetric omega-3 phospholipids affect the microscopic organization of lipid bilayers differently. Cholesterol does not segregate from polyunsaturated phospholipids and, through interactions with them, is able to suppress the formation of non-lamellar structures induced by the symmetric polyunsaturated lipid. However, this order/disorder balance leads to a bilayer whose structural organization cannot be ascribed to either a liquid ordered or to a canonical liquid disordered phase, in that it displays a very loose packing of the intermediate segments of lipid chains.     

PAN基原丝预氧丝炭纤维微结构的几种新的X射线衍射散射定量表征技术

介绍几种新近开发的针对PAN基原丝、预氧丝和炭丝微结构和结构缺陷的X射线衍射与散射定量表征技术,它们是:纤维材料中柱状(椭球状)纳米取向微孔体积分数X射线小角散射定量表征方法;纤维材料中柱状(椭球状)纳米取向微孔取向角的小角散射定量表征方法;预氧丝中链状相与环化相体积分数的小角衍射定量表征方法以及炭纤维中乱层石墨相与单层石墨相的X射线广角衍射定量表征方法等。     

Short-Period GaMnAs/GaAs Superlattices: Optical and Magnetic Characterization

Superlattices with magnetic layers containing from 8 to 16 GaMnAs monolayers corresponding to the mixed crystal composition between 5 and 6% of Mn and from 4 to 10 GaAs monolayers were grown by the low temperature MBE technique and characterized by Raman scattering. Folded acoustic phonons were observed for all superlattices in the Raman scattering spectra. The interlayer exchange coupling, found previously by the wide-angle neutron diffraction in selected superlattices was investigated by the spin-polarized neutron reflectometry. It was always of ferromagnetic type, no trace of antiferromagnetic coupling was found.     

基于RGBD数据与深度权重的压缩光场显示

使用多层空间光调制器的压缩光场(compressive light field,CLF) 显示技术具有空间带宽利用率高、图像分辨率好等优势,是一种很有前景的光场显示技术。传统的方法将光场分解视为超定问题并使用优化算法求解。随着重建光场的分辨率、深度、视角等性能参数的提升,优化算法的计算效率低、内存消耗大的缺陷被放大,难以实现快速计算。为此,本文提出了一种新型CLF分解算法,该算法将原始光场分解为物点进行存储,仅占用少量内存;利用深度权重和加权平均代替了优化算法,大幅提高了运算效率。在同等条件下,所提出的算法占用内存仅是传统方法的38.8%,计算时间缩短了93.7%,图像质量提高约1 dB。最后通过仿真和实验两种方式对比了该方法和传统方法的显示效果,验证了算法的有效性。     

基于雾气特征与不等关系约束的去雾方法

针对现有去雾算法未充分考虑图像雾气信息、复原图像细节模糊等问题,提出一种新颖的反映图像雾信息分布的雾气特征图,并采用不等关系约束方法提高图像质量。首先,提取退化图像的极值通道以实现雾气信息的粗略估计,并通过L-1正则化对其进行优化从而得到雾气特征图。其次,提出一种基于雾气特征的初级大气光幕函数,通过对颜色通道和大气光幕作深入分析,利用均值不等式获得约束后的退化场景大气光幕。最后,利用雾气特征图对局部大气光进行改进,并基于大气散射模型实现图像去雾。将所提算法在真实雾图和合成数据集雾图上与其他经典方法进行比较分析,可以发现,所提算法在单幅图像去雾中展现了较好的性能,且在夜间雾图复原中更具优势。     

Light Spectral Composition Modifies Polyamine Metabolism in Young Wheat Plants

Although light-emitting diode (LED) technology has extended the research on targeted photomorphogenic, physiological, and biochemical responses in plants, there is not enough direct information about how light affects polyamine metabolism. In this study, the effect of three spectral compositions (referred to by their most typical characteristic: blue, red, and the combination of blue and red [pink] lights) on polyamine metabolism was compared to those obtained under white light conditions at the same light intensity. Although light quality induced pronounced differences in plant morphology, pigment contents, and the expression of polyamine metabolism-related genes, endogenous polyamine levels did not differ substantially. When exogenous polyamines were applied, their roborative effect were detected under all light conditions, but these beneficial changes were correlated with an increase in polyamine content and polyamine metabolism-related gene expression only under blue light. The effect of the polyamines on leaf gene expression under red light was the opposite, with a decreasing tendency. Results suggest that light quality may optimize plant growth through the adjustment of polyamine metabolism at the gene expression level. Polyamine treatments induced different strategies in fine-tuning of polyamine metabolism, which were induced for optimal plant growth and development under different spectral compositions.