Beatriz Tlatelpa-Romero  Verna Czares-Ordoez  Luis F. Oyarzbal  Luis G. Vzquez-de-Lara 《International journal of molecular sciences》2023,24(1)

Diffuse parenchymal lung diseases (DPLD) or Interstitial lung diseases (ILD) are a heterogeneous group of lung conditions with common characteristics that can progress to fibrosis. Within this group of pneumonias, idiopathic pulmonary fibrosis (IPF) is considered the most common. This disease has no known cause, is devastating and has no cure. Chronic lesion of alveolar type II (ATII) cells represents a key mechanism for the development of IPF. ATII cells are specialized in the biosynthesis and secretion of pulmonary surfactant (PS), a lipid-protein complex that reduces surface tension and minimizes breathing effort. Some differences in PS composition have been reported between patients with idiopathic pulmonary disease and healthy individuals, especially regarding some specific proteins in the PS; however, few reports have been conducted on the lipid components. This review focuses on the mechanisms by which phospholipids (PLs) could be involved in the development of the fibroproliferative response.  相似文献   

    

Georgios Tsakonas  Andreas Koulouris  Dominika Kazmierczak  Johan Botling  Cristian Ortiz-Villalon  Helena Nord  Magnus Lindskog  Martin Sandelin  Patrick Micke  Per Hydbring  Simon Ekman 《International journal of molecular sciences》2023,24(1)

Distant spreading of tumor cells to the central nervous system in non-small cell lung cancer (NSCLC) occurs frequently and poses major clinical issues due to limited treatment options. RNAs displaying differential expression in brain metastasis versus primary NSCLC may explain distant tumor growth and may potentially be used as therapeutic targets. In this study, we conducted systematic microRNA expression profiling from tissue biopsies of primary NSCLC and brain metastases from 25 patients. RNA analysis was performed using the nCounter Human v3 miRNA Expression Assay, NanoString technologies, followed by differential expression analysis and in silico target gene pathway analysis. We uncovered a panel of 11 microRNAs with differential expression and excellent diagnostic performance in brain metastasis versus primary NSCLC. Five microRNAs were upregulated in brain metastasis (miR-129-2-3p, miR-124-3p, miR-219a-2-3p, miR-219a-5p, and miR-9-5p) and six microRNAs were downregulated in brain metastasis (miR-142-3p, miR-150-5p, miR-199b-5p, miR-199a-3p, miR-199b-5p, and miR-199a-5p). The differentially expressed microRNAs were predicted to converge on distinct target gene networks originating from five to twelve core target genes. In conclusion, we uncovered a unique microRNA profile linked to two target gene networks. Our results highlight the potential of specific microRNAs as biomarkers for brain metastasis in NSCLC and indicate plausible mechanistic connections.  相似文献   

    

Xiang Wang  Jae‐Hyeok Lee  Ruibin Li  Yu‐Pei Liao  Joohoon Kang  Chong Hyun Chang  Linda M. Guiney  Vahid Mirshafiee  Linjiang Li  Jianqin Lu  Tian Xia  Mark C. Hersam  André E. Nel 《Small (Weinheim an der Bergstrasse, Germany)》2018,14(23)

Carbon nanotubes (CNTs) exhibit a number of physicochemical properties that contribute to adverse biological outcomes. However, it is difficult to define the independent contribution of individual properties without purified materials. A library of highly purified single‐walled carbon nanotubes (SWCNTs) of different lengths is prepared from the same base material by density gradient ultracentrifugation, designated as short (318 nm), medium (789 nm), and long (1215 nm) SWCNTs. In vitro screening shows length‐dependent interleukin‐1β (IL‐1β) production, in order of long > medium > short. However, there are no differences in transforming growth factor‐β1 production in BEAS‐2B cells. Oropharyngeal aspiration shows that all the SWCNTs induce profibrogenic effects in mouse lung at 21 d postexposure, but there are no differences between tube lengths. In contrast, these SWCNTs demonstrate length‐dependent antibacterial effects on Escherichia coli, with the long SWCNT exerting stronger effects than the medium or short tubes. These effects are reduced by Pluronic F108 coating or supplementing with glucose. The data show length‐dependent effects on proinflammatory response in macrophage cell line and antibacterial effects, but not on collagen deposition in the lung. These data demonstrate that over the length scale tested, the biological response to highly purified SWCNTs is dependent on the complexity of the nano/bio interface.  相似文献   

    

Wei Cheng  Chaoyu Liang  Lv Xu  Gan Liu  Nansha Gao  Wei Tao  Lingyan Luo  Yixiong Zuo  Xusheng Wang  Xudong Zhang  Xiaowei Zeng  Lin Mei 《Small (Weinheim an der Bergstrasse, Germany)》2017,13(29)

A nanocarrier system of d ‐a‐tocopheryl polyethylene glycol 1000 succinate (TPGS)‐functionalized polydopamine‐coated mesoporous silica nanoparticles (NPs) is developed for sustainable and pH‐responsive delivery of doxorubicin (DOX) as a model drug for the treatment of drug‐resistant nonsmall cell lung cancer. Such nanoparticles are of desired particle size, drug loading, and drug release profile. The surface morphology, surface charge, and surface chemical properties are also successfully characterized by a series of techniques such as transmission electron microscopy (TEM), X‐ray photoelectron spectroscopy (XPS), Brunauer‐Emmett‐Teller (BET) method, thermal gravimetric analysis (TGA), dynamic light scattering (DLS), and Fourier transform infrared spectroscopy (FTIR). The normal A549 cells and drug‐resistant A549 cells are employed to access the cytotoxicity and cellular uptake of the NPs. The therapeutic effects of TPGS‐conjugated nanoparticles are evaluated in vitro and in vivo. Compared with free DOX and DOX‐loaded NPs without TPGS ligand modification, MSNs‐DOX@PDA‐TPGS exhibits outstanding capacity to overcome multidrug resistance and shows better in vivo therapeutic efficacy. This splendid drug delivery platform can also be sued to deliver other hydrophilic and hydrophobic drugs.  相似文献   

    

Siobhan M. O'Sullivan  Tomas Lafarga  Maria Hayes  Nora M. O'Brien 《Journal of Food Biochemistry》2017,41(6)

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Mostafa Asadzade  Amir Shamloo 《国际能源研究杂志》2017,41(12):1730-1739

Finding the optimal flow pattern in bipolar plates of a proton exchange membrane is a crucial step for enhancing the performance of the device. This design plays a critical role in fluid mass transport through microporous layers, charge transfer through conductive media, management of the liquid water produced in microchannels, and microporous layers and heat management in fuel cells. This article investigates different types of common flow patterns in bipolar plates while considering a uniform pressure and velocity distribution as well as a uniform distribution of reactants through all the surfaces of the catalyst layer as the design criteria so that there would be a consistent electron production by the catalyst layer. Then, by identifying the important parameters in achieving the best performance of a fuel cell, a microfluidic flow pattern is inspired from the lungs in the human body, and an innovative bipolar plate is suggested, which was not proposed before. Afterwards, numerical simulations were carried out using computational fluid dynamics methods, and the mentioned bipolar plate called lung‐shaped bipolar plate was modeled. Simulations in this research showed that the lung‐shaped microfluidic flow pattern is an appropriate flow pattern to gain maximum power and energy density. In other words, the best polarization curve and power density curve are obtained by using the lung‐shaped bipolar plate in a proton exchange membrane fuel cell compared with previously suggested patterns. Copyright © 2017 John Wiley & Sons, Ltd.  相似文献   

    

Ge Zhan  Leiqing Pan  Kang Tu  Shunshan Jiao 《Journal of food science》2016,81(10):H2578-H2586

The preparation, quantification, and characterization of flavonoid compounds from Chinese water chestnut peel (CWCP) flavonoid extract and ethyl acetate fraction (EF), n‐butanol fraction, and water fraction were studied. Among these, EF showed the maximum free radical levels (IC₅₀ values of 0.36, 0.40, and 0.37 mg/mL for DPPH?, ABTS? ⁺ , and ?OH, respectively), nitrite scavenging effects (IC₅₀ = 1.89 mg/mL), and A549 cell inhibitory activities (IC₅₀ = 776.12 μg/mL) with the highest value of total flavonoid content (TFC, 421.32 mg/g). Moreover, the contents of 8 flavonoids in this fraction were quantified using high‐performance liquid chromatography, and fisetin, diosmetin, luteolin, and tectorigenin were the 4 major flavonoids with levels of 31.66, 29.91, 13.69, and 12.41 mg/g, respectively. Luteolin produced a greater inhibition of human lung cancer A549 cells (IC₅₀ = 59.60 μg/mL) than did fisetin, diosmetin, and tectorigenin. Flow cytometry revealed that the cellular mechanisms of luteolin inhibition of A549 cells were achieved via the induction of cell proliferation arrest at G₁ phase and apoptosis/necrosis. Our findings suggest that flavonoids are closely associated with antitumor, antioxidant, and nitrite scavenging effects of CWCP.  相似文献   

    

Xinyu Zhang;Chenyu Zhu;Luyun Zhang;Luyi Tan;Wenli Cheng;Min Li;Xingtan Zhang;Wenjuan Zhang;Wenji Zhang; 《Food Science & Nutrition》2024,12(11):9238-9251

Resveratrol, a natural compound found in various plants, is known for its anti-inflammatory, antioxidant, and senescence-delaying properties. RNA N6-methyladenosine (m6A) methylation plays a crucial role in oxidative stress and premature cellular senescence processes and is closely associated with age-related disorders. However, the anti-premature senescence via RNA m6A methylation mechanism of resveratrol is still not fully understood. In this study, based on premature senescence model of human embryonic lung fibroblasts (HEFs) induced by hydrogen peroxide (H2O2), a widely accepted model of premature senescence caused by oxidative stress, we explored the anti-aging regulatory effects of resveratrol at the RNA m6A methylation level. Our data suggested that resveratrol significantly delayed premature senescence by increasing cell viability, reducing SA-β-gal blue staining rate, ROS levels, and senescence-associated secretory phenotypes (SASP) expression in HEFs. Meanwhile, resveratrol increased the whole RNA methyltransferases activity and the overall m6A level during senescence. Furthermore, three genes CCND2, E2F1, and GADD45B have been identified as the main ones regulating premature by resveratrol. Specifically, it decreased E2F1, GADD45B RNA m6A methylation level, and increased CCND2 level in premature cells. Our study provided new clues for exploring the mechanism and application of resveratrol in the field of premature aging.  相似文献   

    

Chunyan Min  Hui Lu  Qirun Li  Jie Yuan  Chen Chen  Heran Li  Jian Zhang 《Journal of the science of food and agriculture》2018,98(12):4420-4426

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Luna Rahman  Andrew Williams  Krishna Gelda  Jake Nikota  Dongmei Wu  Ulla Vogel  Sabina Halappanavar 《Small (Weinheim an der Bergstrasse, Germany)》2020,16(36)

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