Cobalt Ferrite Magnetic Nanoparticles for Tracing Mesenchymal Stem Cells in Tissue: A Preliminary Study

Therapy with mesenchymal stem cells (MSCs) is promising in many diseases. Evaluation of their efficacy depends on adequate follow-up of MSCs after transplantation. Several studies have shown that MSCs can be labeled and subsequently visualized with magnetic nanoparticles (NPs). We investigated the homing of MSCs labeled with magnetic cobalt ferrite NPs in experimentally induced acute kidney injury in mice. To explore the homing of MSCs after systemic infusion into mice, we developed a pre-infusion strategy for optimal tracing and identification of MSCs with polyacrylic acid-coated cobalt ferrite (CoFe₂O₄) NPs by light and transmission electron microscopy (TEM) in various organs of mice with cisplatin-induced acute kidney injury and control mice. By correlative microscopy, we detected MSCs labeled with NPs in the lungs, spleen, kidney, and intestine of cisplatin-treated mice and in the lungs and spleen of control mice. Our results confirm that labeling MSCs with metal NPs did not affect the ultrastructure of MSCs and their ability to settle in various organs. This study demonstrates the usefulness of cobalt ferrite NPs in ex vivo visualization of MSCs and offers correlative microscopy as a useful method in routine histopathology laboratories for tracing MSCs in paraffin-embedded tissue.     

中空海胆状结构的碳包覆Fe2O3用作锂离子电池的高性能负极材料

Fe2O3由于成本低廉,储量丰富和理论比容量高(1007 mA hg^-1)等特点,在锂离子电池负极材料的应用中极具发展前景.然而一些问题仍然存在,如:充放电过程中比容量的迅速衰减,不可逆的体积膨胀以及较短的循环寿命等.这些问题严重制约了Fe2O3在锂离子电池中的实际应用.为了突破这些局限,本文以金属-有机骨架(MOF...     

Induced Zinc Loss Produces Heterogenous Biological Responses in Melanoma Cells

Zinc levels in serum and/or tissue are reported to be altered in melanoma with unknown effects on melanoma development and biology. The purpose of this study was to examine the effects of acute chelation of free intracellular zinc pools in melanoma cell lines Bowes and A375, as well as selected melanoma tissue explants with high or low intracellular free zinc. Zinc chelating agent TPEN at the concentration of 25 µM was employed during 48 h, which significantly reduced intracellular free zinc while decreasing melanoma cell proliferation, inducing G1/S arrest and cell damage leading to mitochondrial, caspase-dependent apoptosis. Chelation of free zinc was also associated with increased generation of superoxide in cell lines but not marked lysosomal membrane damage. Conversely, melanoma explant cultures mostly displayed time-dependent loss of lysosomal membrane integrity in the presence of slowly growing superoxide levels. Loss of free zinc-dependent p53 activity was similarly disparate in individual melanoma models. Surviving melanoma cells were arrested in the cell cycle, and varying proportions of them exhibited features characteristic of premature senescence, which increased in time despite zinc reloading. The present results show that melanoma cells with varying free zinc levels respond to its acute loss in a number of individual ways, reflecting activated mechanisms including oxidative stress, lysosomal damage, and p53 activity leading to heterogenous outcomes including cell death, transient, and/or permanent cell cycle arrest and premature senescence.     

氨浸工艺从废旧锂电池中选择性回收有价金属

研究了碳酸氢铵-还原剂体系选择性浸出废旧三元锂电池中锂、镍、钴的过程。考察了浸出温度、碳酸氢铵浓度、还原剂的种类和浓度、固液比及浸出时间等对有价金属浸出率的影响,并通过XRD、SEM-EDS和FT-IR等表征方法对选择性浸出机理进行了初步探明。结果表明：在浸出温度80 ℃、浸出时间2.5 h、碳酸氢铵浓度2.5 mol/L、固液比50 g/L、还原剂亚硫酸钠浓度0.6 mol/L的条件下,锂、镍、钴的浸出率分别为96.86%、96.36%、93.43%,而锰几乎不被浸出。碳酸氢铵-亚硫酸钠还原浸出体系可以实现从废旧三元锂电池材料中高效、选择性回收锂、镍和钴。     

超导磁体脉冲冲击下MOV的破坏特性微观分析

为了将氧化锌电阻阀片(MOV)用于超导磁体的保护,研究了MOV在高温超导磁体产生的脉冲下的冲击破坏过程中的电气特性变化;还通过破坏后MOV的金相分析、SEM微观分析以及成分分析,研究了MOV破坏前后的微观特性的变化;最后讨论了MOV在超导磁体脉冲破坏下的机理,并针对MOV用于在超导磁体保护提出了改进建议,认为保护用阀片应该采取低压大通流氧化锌压敏陶瓷材料,且应该在配方确定的基础上,探索先进的工艺技术改善阀片的性能。     

用MasterCAM加工双金属材料链轮硬齿表面

运用MasterCAM软件对双金属材料链轮硬齿表面进行曲面三维造型,编程,并对链轮双金属材料硬齿表面进行曲面合理工艺设计,加工出了符合图纸要求的产品零件。阐述了MasterCAM软件在加工实例产品时编程的基本步骤,重点突出了其加工工艺和加工参数等关键问题。     

现行焦炭强度指标在太钢高炉的适用性探讨

重点论述了焦炭反应性在高炉碱金属循环富集条件下的变化,并根据国内外研究提出的反应性会发生逆转的概念,分析了太钢高炉生产的实际情况,明确了现行焦炭强度指标对太钢高炉生产的指导意义。     

有色金属新材料项目风险管理探析

从安徽鑫科新材料股份有限公司面临的实际问题出发。通过建立系统模型,研究有色金属新材料项目的风险管理问题。     

金属镁中锌的测定

根据国际市场对金属镁的特殊要求,笔者探索出了一套金属镁中锌的检测方法。该方法符合国际要求,而且具有准确、高效的特点。     

中国石化工业的发展需要石油冶金技术的支持

中国陆壳的特殊性,决定了中国石油的特殊性。中国陆壳面积小,四面受挤,火山活动与新构造活动活跃。因此,在中国石油中含有丰富的地球深部物质,尤应指出的是来自核幔的元素,其中各类金属元素应予特别的关注。提出了开展石油冶金工业的理论研究与技术攻关研究的建议,这一构想的实现将会大大提高中国石油的经济价值,弥补矿产资源短缺的形势,使石油化工可持续发展走出新路。