Uterine Deletion of Bmal1 Impairs Placental Vascularization and Induces Intrauterine Fetal Death in Mice

Recently, it was demonstrated that the expression of BMAL1 was decreased in the endometrium of women suffering from recurrent spontaneous abortion. To investigate the pathological roles of uterine clock genes during pregnancy, we produced conditional deletion of uterine Bmal1 (cKO) mice and found that cKO mice could receive embryo implantation but not sustain pregnancy. Gene ontology analysis of microarray suggested that uterine NK (uNK) cell function was suppressed in cKO mice. Histological examination revealed the poor formation of maternal vascular spaces in the placenta. In contrast to WT mice, uNK cells in the spongiotrophoblast layer, where maternal uNK cells are directly in contact with fetal trophoblast, hardly expressed an immunosuppressive NK marker, CD161, in cKO mice. By progesterone supplementation, pregnancy could be sustained until the end of pregnancy in some cKO mice. Although this treatment did not improve the structural abnormalities of the placenta, it recruited CD161-positive NK cells into the spongiotrophoblast layer in cKO mice. These findings indicate that the uterine clock system may be critical for pregnancy maintenance after embryo implantation.     

基于BLE4.0姿态解算的无线语音鼠标设计

设计了一款残疾人专用智能无线语音鼠标,让双手不灵活的残疾人灵活地操纵电脑成为现实.本设计发送端采用BLE4.0技术负责头部姿态解算、语音识别、向电脑发送操作指令等功能.无需任何接收装置,只需与电脑自带的蓝牙设备配对后便可通过相应的蓝牙协议对电脑直接进行操控.     

用Visual Basic语言实现模拟绘图功能

本文介绍在Visual Basic语言编程环境中,利用鼠标mousedown、mousemove和mouseup事件,建立一个可供写字或绘图的窗口,将其编译成在桌面上可执行的文件,代替教学用粉笔写字或绘图.     

混合菌种发酵酸马乳的降血脂功能研究

为研究混合菌种发酵酸马乳的降血脂作用,采用混合菌种发酵酸马乳对小鼠进行实验,研究其对高脂模型小鼠体重、血脂水平、脏器指数和动脉硬化（AI）指数的影响。结果表明,混合菌种发酵酸马乳中、高剂量能够显著降低高血脂症小鼠TC、TG、LDL-C、AI水平（p<0.05）,显著升高HLD-C水平（p<0.05）,并且能降低小鼠体重和肝脏指数,说明混合菌种发酵酸马乳对实验性高脂血症小鼠具有降低血脂的作用,对高脂血症及动脉粥样硬化具有一定的预防作用。      

羊栖菜多糖对小鼠抗疲劳作用的研究

研究羊栖菜多糖对小鼠的抗疲劳作用,对于开发利用羊栖菜资源具有重要意义。通过对小鼠灌胃不同剂量的羊栖菜多糖(50、150mg/mL·d),经15d后测定小鼠体重、负重游泳力竭时间、超氧化物歧化酶(SOD)、丙二醛(MDA)、肝糖原、肌乳酸及血清尿素的含量,研究羊栖菜多糖抗疲劳作用机理。结果表明,羊栖菜多糖对小鼠体重增长无明显影响(p>0.05),但能明显延长小鼠负重游泳时间(p<0.01),增强小鼠血清SOD活性(p<0.01),提高肝糖原含量(p<0.01),降低血清MDA(p<0.05)、肌乳酸(p<0.01、0.05)及血清尿素(p<0.01)的含量。说明羊栖菜多糖具有抗疲劳的作用。      

不同发酵天数的芝芪菌质对免疫抑制小鼠免疫功能的影响

为筛选较佳的芝芪菌质发酵时间,本实验通过小鼠碳粒廓清实验,鸡红细胞吞噬功能实验,小鼠血清溶血素、Ig M和Ig G的含量检测,以及小鼠脾淋巴细胞增殖实验,考察了不同发酵天数的芝芪菌质对免疫抑制小鼠非特异性和特异性免疫功能的影响。结果表明:发酵25、30 d的芝芪菌质具有显著的免疫增强功效(p<0.05,0.01;0.05),提示可有效增强免疫功能低下人群的机体免疫力。      

实验小鼠运动参数的模板匹配及粒子滤波提取方法

实验小鼠是一种变形体对象,现有方法难以从连续视频图像中同时提取出运动轨迹和体态细节.本文采用模板匹配和粒子滤波的目标跟踪方法求解这一问题.提出了一种几何体部件模型,在引入小鼠移动速率的基础上给出了其运动状态方程,以二值化前景像素与几何部件模型间的差异度方程为观测模型,以状态方程及相互独立的多维随机变量为运动模型,从而建立起基本粒子滤波算法.与逐帧差分识别方法的对比实验研究表明,所提出的模型与实验小鼠形体相似,能够达到视频在线提取的计算效率.新方法在强噪声干扰条件下解决了运动轨迹和体态同时精确估计,并有效避免了首尾识别混淆及虚影干扰等困境,从而为后续生物学行为分析提供依据.     

基于SolidWorks的鼠标壳注射模设计及模流分析

通过对鼠标壳制件进行工艺分析及注射模的模流分析,最终完成了注射模的结构设计。具体介绍了应用MoldFlow Plastics Insight（MPI）件进行充模、冷却、翘曲等方面的分析过程及应用三雏CAD软件SolidWorks进行模具设计的具体过程。     

Glia in Neurodegeneration: The Housekeeper,the Defender and the Perpetrator

Over the past decade, research has unveiled the intimate relationship between neuroinflammation and neurodegeneration. Microglia and astrocytes react to brain insult by setting up a multimodal inflammatory state and act as the primary defenders and executioners of neuroinflammatory structural and functional changes. Microglia and astrocytes also play critical roles in the maintenance of normal brain function. This intricate balance of homeostatic and neuroinflammatory functions can influence the onset and the course of neurodegenerative diseases. The emergent role of the microglial-astrocytic axis in neurodegenerative disease presents many druggable targets that may have broad therapeutic benefits across neurodegenerative disease. Here, we provide a brief review of the basal function of both microglia and astrocytes, how they are changed in disease states, the significant differences between mouse and human glia, and use of human induced pluripotent stem cells derived from patients to study cell autonomous changes in human astrocytes and microglia.     

Chronic Oral Selegiline Treatment Mitigates Age-Related Hearing Loss in BALB/c Mice

Age-related hearing loss (ARHL), a sensorineural hearing loss of multifactorial origin, increases its prevalence in aging societies. Besides hearing aids and cochlear implants, there is no FDA approved efficient pharmacotherapy to either cure or prevent ARHL. We hypothesized that selegiline, an antiparkinsonian drug, could be a promising candidate for the treatment due to its complex neuroprotective, antioxidant, antiapoptotic, and dopaminergic neurotransmission enhancing effects. We monitored by repeated Auditory Brainstem Response (ABR) measurements the effect of chronic per os selegiline administration on the hearing function in BALB/c and DBA/2J mice, which strains exhibit moderate and rapid progressive high frequency hearing loss, respectively. The treatments were started at 1 month of age and lasted until almost a year and 5 months of age, respectively. In BALB/c mice, 4 mg/kg selegiline significantly mitigated the progression of ARHL at higher frequencies. Used in a wide dose range (0.15–45 mg/kg), selegiline had no effect in DBA/2J mice. Our results suggest that selegiline can partially preserve the hearing in certain forms of ARHL by alleviating its development. It might also be otoprotective in other mammals or humans.