Real time monitoring of bioreactor mAb IgG3 cell culture process dynamics via Fourier transform infrared spectroscopy: Implications for enabling cell culture process analytical technologyŽ 

Compared to small molecule process analytical technology (PAT) applications, biotechnology product PAT applications have certain unique challenges and opportunities. Understanding process dynamics of bioreactor cell culture process is essential to establish an appropriate process control strategy for biotechnology product PAT applications. Inline spectroscopic techniques for real time monitoring of bioreactor cell culture process have the distinct potential to develop PAT approaches in manufacturing biotechnology drug products. However, the use of inline Fourier transform infrared (FTIR) spectroscopic techniques for bioreactor cell culture process monitoring has not been reported. In this work, real time inline FTIR Spectroscopy was applied to a lab scale bioreactor mAb IgG3 cell culture fluid biomolecular dynamic model. The technical feasibility of using FTIR Spectroscopy for real time tracking and monitoring four key cell culture metabolites (including glucose, glutamine, lactate, and ammonia) and protein yield at increasing levels of complexity (simple binary system, fully formulated media, actual bioreactor cell culture process) was evaluated via a stepwise approach. The FTIR fingerprints of the key metabolites were identified. The multivariate partial least squares (PLS) calibration models were established to correlate the process FTIR spectra with the concentrations of key metabolites and protein yield of in-process samples, either individually for each metabolite and protein or globally for all four metabolites simultaneously. Applying the 2^nd derivative pre-processing algorithm to the FTIR spectra helps to reduce the number of PLS latent variables needed significantly and thus simplify the interpretation of the PLS models. The validated PLS models show promise in predicting the concentration profiles of glucose, glutamine, lactate, and ammonia and protein yield over the course of the bioreactor cell culture process. Therefore, this work demonstrated the technical feasibility of real time monitoring of the bioreactor cell culture process via FTIR spectroscopy. Its implications for enabling cell culture PAT were discussed.     

基于BLE4.0姿态解算的无线语音鼠标设计

设计了一款残疾人专用智能无线语音鼠标,让双手不灵活的残疾人灵活地操纵电脑成为现实.本设计发送端采用BLE4.0技术负责头部姿态解算、语音识别、向电脑发送操作指令等功能.无需任何接收装置,只需与电脑自带的蓝牙设备配对后便可通过相应的蓝牙协议对电脑直接进行操控.     

3D图形环境中的鼠标精确定位的研究

在三维图形环境以及其他的三维软件系统中,如何选取三维图形空间中的物体是设计三维图形环境的一个关键点。选取的精确程度往往决定了一个三维图形环境作图质量的高低。阐述了在三维图形环境软件设计中通过光线跟踪的方法来选取三维空间中的几何物体的方法,通过这种方法可以精确并方便地选择三维空间中的物体,在实际的工程应用中取得了很好的效果。     

Systematic robustness analysis of least squares mobile robot velocity estimation using a regular polygonal optical mouse array

This paper presents the robust velocity estimation of an omnidirectional mobile robot using a regular polygonal array of optical mice that are installed at the bottom of a mobile robot. First, the velocity kinematics from a mobile robot to an array of optical mice is derived, from which the least squares estimation of the mobile robot velocity is obtained as the simple average of the optical mouse velocity readings. Second, it is shown that a redundant number of optical mice contributes to the robustness of the least squares mobile robot velocity estimation against both measurement noises and partial malfunction of optical mice. Third, the sensitivity analysis of the least squares mobile robot velocity estimation to imprecise installation of optical mice is made, from which a practical method of optical mouse position calibration is devised. Finally, some experimental results using commercial optical mice are given to demonstrate the validity and performance of the proposed mobile robot velocity estimation. Copyright © 2011 John Wiley and Sons Asia Pte Ltd and Chinese Automatic Control Society     

Enhanced recognition memory following glycine transporter 1 deletion in forebrain neurons.

Selective deletion of glycine transporter 1 (GlyT1) in forebrain neurons enhances N-methyl-D-aspartate receptor (NMDAR)-dependent neurotransmission and facilitates associative learning. These effects are attributable to increases in extracellular glycine availability in forebrain neurons due to reduced glycine re-uptake. Using a forebrain- and neuron-specific GlyT1-knockout mouse line (CamKIIαCre; GlyT1tm1.2fl/fI), the authors investigated whether this molecular intervention can affect recognition memory. In a spontaneous object recognition memory test, enhanced preference for a novel object was demonstrated in mutant mice relative to littermate control subjects at a retention interval of 2 hr, but not at 2 min. Furthermore, mutants were responsive to a switch in the relative spatial positions of objects, whereas control subjects were not. These potential procognitive effects were demonstrated against a lack of difference in contextual novelty detection: Mutant and control subjects showed equivalent preference for a novel over a familiar context. Results therefore extend the possible range of potential promnesic effects of specific forebrain neuronal GlyT1 deletion from associative learning to recognition memory and further support the possibility that mnemonic functions can be enhanced by reducing GlyT1 function. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

鼠笼破碎机的设计与改进

分析原鼠笼破碎机在设计上和生产中存在的问题，通过观察与计算，找出原因并提出改造措施。根据扩产要求，对鼠笼破碎机的结构和各项技术指标进行设计分析，指出影响鼠笼破碎机产能的主要因素，总结出鼠笼破碎机产能的估算公式。     

一种新的鼠标器结构探讨

作为计算机指点式输入设备,目前已有的器械包括:机械式鼠标、光学鼠标、轨迹球、电子笔等。鼠标的应用无疑给人们使用计算机带来了极大便利,但是,由于工作原理的缺陷使得传统鼠标无法应对日益提升的系统应用,比如在三维图形界面下光标定位、控制的实现。本文提出一种新的鼠标器结构,以较小的代价克服了上述传统鼠标器缺点,还实现了三维空间的定位。     

Web-based Biometric Computer Mouse Advisory System to Analyze a User's Emotions and Work Productivity

This article describes the analysis of emotional state and work productivity using a Web-based Biometric Computer Mouse Advisory System to Analyze a User's Emotions and Work Productivity (Advisory system hereafter) developed by this paper's authors. The Advisory system determines the level of emotional state and work productivity integrally by employing three main biometric techniques (physiological, psychological and behavioral). By using these three biometric techniques, the Advisory system can analyze a person's eleven states of being (stress, work productivity, mood, interest in work) and seven emotions (self-control, happiness, anger, fear, sadness, surprise and anxiety) during a realistic timeframe. Furthermore, to raise the reliability of the Advisory system even more, it also integrated the data supplied by the Biometric Finger (blood pressure and pulse rates). Worldwide research includes various scientists who conducted in-depth studies on the different and very important areas of biometric mouse systems. However, biometric mouse systems cannot generate recommendations. The Advisory system determines a user's physiological, psychological and behavioral/movement parameters based on that user's real-time needs and existing situation. It then generates thousands of alternative stress management recommendations based on the compiled Maslow's Pyramid Tables and selects out the most rational of these for the user's specific situation. The information compiled for Maslow's Pyramid Tables consists of a collection of respondent surveys and analyses of the best global practices. Maslow's Pyramid Tables were developed for an employee working with a computer in a typical organization. The Advisory system provides a user with a real-time assessment of his/her own productivity and emotional state. This article presents the Advisory system, a case study and a scenario used to test and validate the developed Advisory system and its composite parts to demonstrate its validity, efficiency and usefulness.     

一种无接触供电的无线鼠标

介绍了一种无接触供电的无线鼠标设计电路,无接触电能发射装置采用USB供电,通过自激振荡电路产生了138 kHz的高频振荡电压;无接触电能接收装置采用感应耦合方式获取电能.无线鼠标用无线模块nRF24E1构成信号发射和接收电路,该电路除具备有线鼠标全部功能外,还可以离开电脑远距离灵活操纵鼠标.     

基于光电传感器的移动机器人室内定位

设计了一种基于光电鼠标和电子罗盘的机器人定位系统.通过放置在小车尾部中央位置的光电鼠标芯片测出X和Y的单位时间内的位移数据,经过STM32处理得到坐标值,并将小车的坐标值等信息显示在液晶屏上.通过电子罗盘得到小车的航向.在获取小车坐标与方向后,上位机通过无线模块向小车发送控制命令,从而控制小车的行驶轨迹,并将行其显示在上位机界面.经实验测试,测量位移的定位误差在0.45％,可以达到满足机器人室内定位系统的要求.