Localizable imaging study for the noninvasive diagnosis of lesions with the backscattering polarized light

The asymmetry appearing in the degree of polarization (DOP) distribution of the backscattering polarized light from tissues is investigated by using polarized Monte Carlo simulation. When the incident point is close to the boundary of the lesion inside the tissue, high asymmetry emerges regardless of the polarized direction of the incident light. A noninvasive method based upon the DOP asymmetry of the backscattering light is proposed to locate lesions hidden in live tissues by scanning a point light source. Imaging of the front projection on complicated lesion structures is demonstrated with this method. Its transverse resolution, which is affected by the wavelength of incident light and the size of the scattering particle, can reach the diameter of the lesion scattering particle theoretically while the best longitudinal detection depth can be achieved by choosing a suitable incident wavelength according to the scattering characters of the tissue.     

Multiplexed-Based Assessment of DNA Damage Response to Chemotherapies Using Cell Imaging Cytometry

The current methods for measuring the DNA damage response (DDR) are relatively labor-intensive and usually based on Western blotting, flow cytometry, and/or confocal immunofluorescence analyses. They require many cells and are often limited to the assessment of a single or few proteins. Here, we used the Celigo^® image cytometer to evaluate the cell response to DNA-damaging agents based on a panel of biomarkers associated with the main DDR signaling pathways. We investigated the cytostatic or/and the cytotoxic effects of these drugs using simultaneous propidium iodide and calcein-AM staining. We also describe new dedicated multiplexed protocols to investigate the qualitative (phosphorylation) or the quantitative changes of eleven DDR markers (H2AX, DNA-PKcs, ATR, ATM, CHK1, CHK2, 53BP1, NBS1, RAD51, P53, P21). The results of our study clearly show the advantage of using this methodology because the multiplexed-based evaluation of these markers can be performed in a single experiment using the standard 384-well plate format. The analyses of multiple DDR markers together with the cell cycle status provide valuable insights into the mechanism of action of investigational drugs that induce DNA damage in a time- and cost-effective manner due to the low amounts of antibodies and reagents required.     

一种针对图像模糊的无参考质量评价指标

在成像模型的基础上,分析了图像模糊的原因,提出了一种为图像构造参考图像的方法,进而将结构相似度(SSIM)评价方法引入到无参考图像质量评价中,提出一种无参考结构清晰度(NRSS)的新的无参考图像质量评价方法,将其用于对模糊图像的质量评价。该方法通过低通滤波器来构造参考图像,通过计算原始图像与参考图像的结构相似度值来评价原始图像质量,很好地结合了成像系统的数学模型和结构相似度评价方法的优势,实验结果表明无参考结构清晰度评价指标能够给出和主观评价方法以及其余有参考评价方法一致的结果。     

Systematic Review: Targeted Molecular Imaging of Angiogenesis and Its Mediators in Rheumatoid Arthritis

Extensive angiogenesis is a characteristic feature in the synovial tissue of rheumatoid arthritis (RA) from a very early stage of the disease onward and constitutes a crucial event for the development of the proliferative synovium. This process is markedly intensified in patients with prolonged disease duration, high disease activity, disease severity, and significant inflammatory cell infiltration. Angiogenesis is therefore an interesting target for the development of new therapeutic approaches as well as disease monitoring strategies in RA. To this end, nuclear imaging modalities represent valuable non-invasive tools that can selectively target molecular markers of angiogenesis and accurately and quantitatively track molecular changes in multiple joints simultaneously. This systematic review summarizes the imaging markers used for single photon emission computed tomography (SPECT) and/or positron emission tomography (PET) approaches, targeting pathways and mediators involved in synovial neo-angiogenesis in RA.     

The Role of the Choroid in Stargardt Disease

Stargardt disease is the commonest juvenile macular dystrophy. It is caused by genetic mutations in the ABCA4 gene. Diagnosis is not always straightforward, and various phenocopies exist. Late-onset disease can be misdiagnosed with age-related macular disease. A correct diagnosis is particularly critical because of emergent gene therapies. Stargardt disease is known to affect retinal pigment epithelium and photoreceptors. Many studies have also highlighted the importance of the choroid in the diagnosis, pathophysiology, and progression of the disease. The choroid is in an integral relationship with the retinal pigment epithelium and photoreceptors, and its possible involvement during the disease should be considered. The purpose of this review is to analyze the current diagnostic tools for choroidal evaluation and the extrapolation of useful data for ophthalmologists and researchers studying the disease.     

Tumor-Localized Administration of α-GalCer to Recruit Invariant Natural Killer T Cells and Enhance Their Antitumor Activity against Solid Tumors

Invariant natural killer T (iNKT) cells have the capacity to mount potent anti-tumor reactivity and have therefore become a focus in the development of cell-based immunotherapy. iNKT cells attack tumor cells using multiple mechanisms with a high efficacy; however, their clinical application has been limited because of their low numbers in cancer patients and difficulties in infiltrating solid tumors. In this study, we aimed to overcome these critical limitations by using α-GalCer, a synthetic glycolipid ligand specifically activating iNKT cells, to recruit iNKT to solid tumors. By adoptively transferring human iNKT cells into tumor-bearing humanized NSG mice and administering a single dose of tumor-localized α-GalCer, we demonstrated the rapid recruitment of human iNKT cells into solid tumors in as little as one day and a significantly enhanced tumor killing ability. Using firefly luciferase-labeled iNKT cells, we monitored the tissue biodistribution and pharmacokinetics/pharmacodynamics (PK/PD) of human iNKT cells in tumor-bearing NSG mice. Collectively, these preclinical studies demonstrate the promise of an αGC-driven iNKT cell-based immunotherapy to target solid tumors with higher efficacy and precision.     

影像设备OM测试法解读(能源之星2.0版)

美国能源之星对影像设备能效测试2.0版操作模式功率法(OM法)主要反应被测样品在完成准备状态、睡眠状态、低耗能状态或关闭状态的能耗情况。分析了OM法的各测试项目以及相应指标,归纳总结了该标准规范在实施中应注意的问题,对影像设备附加功能进行了详细说明。     

虚拟现实系统中的视觉延迟研究

视觉延迟将致虚拟现实系统的沉浸感遭到破坏，并导致用户产生运动高，对产生视觉延迟的检测延迟和成像延迟进行了研究。     

星载-机载混合双站SAR回波信号的仿真

对于星载-机载双站合成孔径雷达（SA-BiSAR）系统,回波模拟的关键是计算出卫星、飞机和目标这三者之间的距离。在考虑地球自转的情况下,提出了用坐标变换的方法,解决了近地空间与外太空之间距离的计算问题,实现了任意几何模型下更为精确的SA-BiSAR回波模拟。同时,利用此方法也可获得直达波信号。点目标的仿真展示了SAR回波信号的特征,该方法得到了验证。     

New Developments in Carbonic Anhydrase IX-Targeted Fluorescence and Nuclear Imaging Agents

Carbonic anhydrase IX (CAIX) is a tumor-specific and hypoxia-induced biomarker for the molecular imaging of solid malignancies. The nuclear- and optical-imaging of CAIX-expressing tumors have received great attention due to their potential for clinical applications. Nuclear imaging is a powerful tool for the non-invasive diagnosis of primary and metastatic CAIX-positive tumors and for the assessment of responses to antineoplastic treatment. Intraoperative optical fluorescence imaging provides improved visualization for surgeons to increase the discrimination of tumor lesions, allowing for safer surgical treatment. Over the past decades, many CAIX-targeted molecular imaging probes, based on monoclonal antibodies, antibody fragments, peptides, and small molecules, have been reported. In this review, we outline the recent development of CAIX-targeted probes for single-photon emission computerized tomography (SPECT), positron emission tomography (PET), and near-infrared fluorescence imaging (NIRF), and we discuss issues yet to be addressed.