Truncated O-Glycan-Bearing MUC16 Enhances Pancreatic Cancer Cells Aggressiveness via α4β1 Integrin Complexes and FAK Signaling

Elevated levels of Mucin-16 (MUC16) in conjunction with a high expression of truncated O-glycans is implicated in playing crucial roles in the malignancy of pancreatic ductal adenocarcinoma (PDAC). However, the mechanisms by which such aberrant glycoforms present on MUC16 itself promote an increased disease burden in PDAC are yet to be elucidated. This study demonstrates that the CRISPR/Cas9-mediated genetic deletion of MUC16 in PDAC cells decreases tumor cell migration. We found that MUC16 enhances tumor malignancy by activating the integrin-linked kinase and focal adhesion kinase (ILK/FAK)-signaling axis. These findings are especially noteworthy in truncated O-glycan (Tn and STn antigen)-expressing PDAC cells. Activation of these oncogenic-signaling pathways resulted in part from interactions between MUC16 and integrin complexes (α4β1), which showed a stronger association with aberrant glycoforms of MUC16. Using a monoclonal antibody to functionally hinder MUC16 significantly reduced the migratory cascades in our model. Together, these findings suggest that truncated O-glycan containing MUC16 exacerbates malignancy in PDAC by activating FAK signaling through specific interactions with α4 and β1 integrin complexes on cancer cell membranes. Targeting these aberrant glycoforms of MUC16 can aid in the development of a novel platform to study and treat metastatic pancreatic cancer.     

再生塑料内污染物迁移的有限元分析

建立计算仿真模型,该模型可用来预测再生塑料内污染物向液体产品(如食品)的迁移.该仿真模型的建立是基于有限元方法,可分析多层结构中的迁移.该项工作对于再生塑料尤其是有低阻隔性的聚烯烃在食品包装上的应用具有潜在意义.     

Sanguinarine Inhibition of TNF-α-Induced CCL2, IKBKE/NF-κB/ERK1/2 Signaling Pathway,and Cell Migration in Human Triple-Negative Breast Cancer Cells

Angiogenesis is a process that drives breast cancer (BC) progression and metastasis, which is linked to the altered inflammatory process, particularly in triple-negative breast cancer (TNBC). In targeting inflammatory angiogenesis, natural compounds are a promising option for managing BC. Thus, this study was designed to determine the natural alkaloid sanguinarine (SANG) potential for its antiangiogenic and antimetastatic properties in triple-negative breast cancer (TNBC) cells. The cytotoxic effect of SANG was examined in MDA-MB-231 and MDA-MB-468 cell models at a low molecular level. In this study, SANG remarkably inhibited the inflammatory mediator chemokine CCL2 in MDA-MB-231 and MDA-MB-468 cells. Furthermore, qRT-PCR confirmed with Western analysis studies showed that mRNA CCL2 repression was concurrent with reducing its main regulator IKBKE and NF-κB signaling pathway proteins in both TNBC cell lines. The total ERK1/2 protein was inhibited in the more responsive MDA-MB-231 cells. SANG exhibited a higher potential to inhibit cell migration in MDA-MB-231 cells compared to MDA-MB-468 cells. Data obtained in this study suggest a unique antiangiogenic and antimetastatic effect of SANG in the MDA-MB-231 cell model. These effects are related to the compound’s ability to inhibit the angiogenic CCL2 and impact the ERK1/2 pathway. Therefore, SANG use may be recommended as a component of the therapeutic strategy for TNBC.     

The Migration of Multi-tier E-commerce Applications to an Enterprise Java Environment

As technology evolves, many organizations face the problem of migrating legacy applications from one technology base to another. We report on a case study involving the migration of legacy code into the IBM® WebSphere® Commerce Suite product. Specifically, we focus on the problem of migrating applications that use traditional database access techniques to applications using the Enterprise JavaBean (EJB) programming model. Our results include a practical methodology that facilitates such migration, as well as a tool that supports this methodology. The tool has been released on IBM's alphaWorks site.     

线性调频连续波雷达双通道距离改进系统

线性调频连续波雷达的差拍中频信号包含被探测目标的距离信息.为了更好地从线性调频连续波雷达的同波中提取出较远距离的目标信息,从电磁波在空间传播衰减情况以及脉压信号能量的大小与回波延时时间的关系两个方面,仿真论证了影响线性调频连续波雷达的探测距离的原因,并基于仿真结果提出了一种以DDS为线性调频信号源的双通道线性调频连续波雷达系统.通过用MATLAB软件进行仿真,得到在相同的工作环境下雷达探测距离从传统线性调频连续波雷达系统的45km提高到双通道系统的135km,超出传统线性调频连续波雷达系统距离的2倍以上,对工程实践具有指导意义.     

数据流查询计划的并行迁移策略

数据流中的查询计划需要不断进行适应性优化,针对该特征提出一种查询计划的并行迁移策略。该策略能确保在输出过程中不丢。失元组或产生冗余元组,维持正确的元组输出时序。实验结果证明,该策略可以使查询计划平滑过渡,避免迁移过程出现无元组输出的空自期,在系统资源紧张和数据流流速过大时,维持较少的中间元组数和较大输出速率。     

基于卡尔曼滤波的InSAR柔性基线距离测量

对于柔性长基线构型的双天线InSAR系统,基线矢量动态偏移产生的误差会降低系统的成像质量,并最终影响高程精度。针对单相机和激光测距仪组合测量系统中如何提高基线距离向精度的问题,提出了一种基于卡尔曼滤波的基线距离测量方法。建立了基线距离向抖动数学模型,在此模型基础上提出了基于卡尔曼滤波的动态测量方法,对该方法进行了动态仿真分析。仿真结果表明,在几十米的测量距离内,该方法能够将基线距离向的测量精度降低到亚毫米量级,是测量基线距离的有效方法。     

新型光纤激光雷达的结构理论分析与作用距离计算

为了改善当前激光雷达存在的对振动敏感,体积庞大,对人眼不安全等问题,综合国内外激光雷达研究现状的基础上.给出了一种新型光纤激光雷达的结构.该雷达利用光纤激光器作为光源,光路中大量采用了光纤器件,使得系统体积大大减小.采用相干探测的原理,根据激光雷达作用距离方程,给出了信噪比与光纤激光雷达作用距离的关系.根据所选的器件参数,通过理论计算,在探测概率为0.9,虚警概率小于10-4的情况下,得到了2 km的最大作用距离.所设计的光纤激光雷达体积小,结构紧凑、新颖,具有良好的探测能力.     

一种改进的折半查找算法

针对查找范围变化很大而又相对稳定的查找对象,给出了一种改进的基于区间控制的折半查找算法,当后一个查找对象在前一个查找对象附近时,在最坏状态和平均状态下,该算法与传统的标准折半查找算法相比,其查找长度显著减少,查找速度快,当父表很大而子表相对很小时,存储上仅需增加一个额外的存储单元,实现代价很小。此算法适用于过程控制中的实时查找处理,有一定的实用价值。