人热休克蛋白70基因的克隆、表达及鉴定

目的克隆人热休克蛋白70(HSP70)基因,构建其原核高效表达载体。方法将PCR扩增的HSP70基因克隆到原核高效表达载体pET-28b中,转化大肠杆菌JM109。挑选阳性克隆,提取质粒pE28b70F,转化大肠杆菌BL21(DE3),IPTG诱导表达目标蛋白。结果在大肠杆菌中成功表达了HSP70,表达量约占细菌总蛋白的22·3%,其中可溶性目标蛋白占上清总蛋白的12%。Westernblot表明该目标蛋白具有与鼠抗人HSP70单抗特异结合的抗原活性。通过Ni2+-NTA亲和柱,从1L诱导产物中纯化出约1520mg重组蛋白,纯度在80%以上。结论已成功表达HSP70,为进一步研究其生物学功能和作用机制奠定了基础。     

基于跨文化适应理论的电影《推手》赏析

运用跨文化适应理论对李安编剧并导演的电影《推手》的主人公所经历的文化震荡和跨文化适应过程进行了分析和解读。同时,结合《推手》的创作背景,指出《推手》实为一部跨文化交际的生动教材,不仅深刻描写了跨文化适应过程中人们要经历的情感痛苦和迷茫,而且客观呈现了人们在跨文化适应过程中从深感疏离到渴望融合的心理过程,能为即将体验跨文化适应过程的人们提供情感和心理准备。     

“广岛——日本‘最具活力’的城市”中的文化冲击

从跨文化交际中的文化冲击理论出发,解读＂广岛——日本‘最具活力’的城市＂一文。分析该文作者,一位普通美国记者,在战后重建的广岛所经历的种种不解和困惑。并进一步分析日美两个民族在对待等级秩序和人人平等,自杀是消极还是积极等方面存在的差异。正是这些文化差异导致普通美国民众对日本文化的误读并产生排斥性情感。     

Effects of circumferential configuration concerning splitter blade on the aerodynamic performance in a transonic axial fan

For a certain type of transonic axial fan, the flow field of a fan rotor with splitter blade was computed by numerical simulation, and the shape of the rotor was modified. The effects of different circumferential distributions concerning the splitter cascades upon the aerodynamic performance were investigated. The studies show that the optimum splitter cascade is not very close to the suction side of main blade. The load between the main blade and the splitter blade can be soundly distributed in terms of the adjustment of circumferential position of the splitter blade. The best aerodynamic performance can be successfully obtained according to the optimum shape of the expanding fluid channel reasonably formed by the splitter blade and the main blade.     

Simplified design of rock cavern concrete lining to resist shock loading

A simplified method was proposed for the design of concrete lining in underground rock cavern/tunnel against shock loading. The loading may result from the detonation of explosives on ground surface or ground penetration projectiles exploding adjacent to the cavern/tunnel. The resulting problem necessitates the solution of the dynamics of a beam loaded by a transient pressure uniformly distributed over the span. According to mechanical characteristics of the system with rock bolt and shotcrete, a dynamic support design method based on equivalent single degree of freedom (SDOF) was put forward. The SDOF method was applied to obtaining the maximum displacement at the mid-span of the beam, which is often the controlling factor in the blast-resistant design. In the formulation of the problem, the proposed method combines the phenomena of spalling and structural dynamics theory. An example is provided to demonstrate the applicability of this simplified method.     

Formation mechanism of aerodynamic drag of high-speed train and some reduction measures

Aerodynamic drag is proportional to the square of speed. With the increase of the speed of train, aerodynamic drag plays an important role for high-speed train. Thus, the reduction of aerodynamic drag and energy consumption of high-speed train is one of the essential issues for the development of the desirable train system. Aerodynamic drag on the traveling train is divided into pressure drag and friction one. Pressure drag of train is the force caused by the pressure distribution on the train along the reverse running direction. Friction drag of train is the sum of shear stress, which is the reverse direction of train running direction. In order to reduce the aerodynamic drag, adopting streamline shape of train is the most effective measure. The velocity of the train is related to its length and shape. The outer wind shields can reduce train’s air drag by about 15%. At the same time, the train with bottom cover can reduce the air drag by about 50%, compared with the train without bottom plate or skirt structure. Foundation item: Project(2001AA505000) supported by the National High-Tech Research and Development of China     

基于纯声子散射模型的纳米薄膜热传导分析

起粘结作用的薄膜结构厚度达到纳米尺度时,应用由微尺度观点建立的非傅立叶热传导模型分析其性能。文章采用纯声子散射模型分析绝缘薄膜结构的温度场和热应力,并与用在宏观尺度下的傅立叶热传导模型所得结果进行比较,同时研究绝缘薄膜结构热物理性能参数对温度场和热应力的影响。研究表明：采用纯声子散射模型分析结果与傅立叶热传导模型所得结果有明显区别,绝缘薄膜结构热物理性能参数对温度场和热应力也有显著影响。     

激光冲击LY2铝合金的薄膜性能

脉冲激光冲击LY2铝合金,在试样表面形成约1.7mm的薄膜,研究了薄膜与基体接合处横截面的微观形貌以及激光冲击搭接率和激光冲击次数对薄膜层残余应力的影响。研究结果表明:激光冲击的光斑搭接率对试样横截面的残余应力影响显著;在薄膜层最大残余应力存在于试样的表层或者近表层,深度方向的残余应力值和残余压应力值变化率均与表层深度成反比;多次冲击能够获得较大的残余应力值,但对于激光冲击LY2铝合金薄膜深度影响较小。     

加强素质能力培养，提高学生核心竞争力

高等职业教育既要遵循普通高等教育的一般规律,也要充分体现职业教育的特殊要求,高素质高技能型人才培养应以能力培养为本位,以综合素质培养为基点,专业能力培养为中心,既要重视高素质教育,也要注重职业技能培养,才能真正实现培养“双高”人才的目标。     

网络条件下高职科学教育与人文教育融合的探索

作为现代信息传播的先进媒介,网络已成为高职学生获取信息及进行信息交流的重要途径,进而对高职学生的价值观和行为方式产生了潜移默化的影响。这既给高职院校科学教育与人文教育的融合带来了挑战,同时也为高职院校开展科学教育与人文教育提供了难得的机遇,面对挑战与机遇,高职院校教育工作者应转变观念,采取积极的应对策略,利用网络促进高职科学教育与人文教育的融合,全面提高高职学生的综合素质。