The effect of various carbon compounds on the synthesis of alcohol oxidase in a medium with methanol was studied in the wild type strain of Pichia pinus as well as in gcr1 and ecr1 mutants defective in glucose and ethanol repression of methanol metabolic enzymes, respectively. Compounds repressing the synthesis of alcohol oxidase in the wild type strain were divided into four groups. Repression of alcohol oxidase by compounds of the first group (glucose, fructose, mannose, galactose, L -sorbose and xylose) was impaired only in the gcr1 mutant and that by compounds of the second group (ethanol, acetate, 2-oxoglutarate and erythritol) only in the ecr1 mutant. Repression by compounds of the third group (malate, dihydroxyacetone) was not impaired in both these regulatory mutants and that by compounds of the fourth group (succinate, fumarate, L -arabinose, sorbitol, salicin, xylitol and cellobiose) was partially reduced in both gcr1 and ecr1 strains. Mutation gcr1 causes a significant decrease in phosphofructokinase activity. It also led to a six- to seven-fold increase in intracellular pools of glucose-6-phosphate and fructose-6-phosphate and to a two-fold decrase in the intracellular pool of fructose-1,6-bisphosphate. In ecr1 strains, a decrese in 2-oxoglutarate dehydrogenase activity accompanied by an increae in activities of NAD- and NADP-dependent isocitrate dehydrogenases and NAD- and NADP-dependent glutamate dehydrogenases was demonstrated. The intracellular pool of 2-oxoglutarate was increased 2·5-fold in ecr1 strains. Genes GCR1 and ECR1 are not linked. The mechanisms of catabolite repression of alcohol oxidase in methylotrophic yeasts are discussed. 相似文献
Data from different applications — voice, video, file transfer, interactive — will be multiplexed in the future over broadband integrated services digital networks (BISDN). Data are segmented into 48-byte blocks prefixed by a 5-byte header and transported over the network using the asynchronous transfer mode (ATM).
An ATM connection-request is a contract between the user and the network; the user specifies a rate requirement, delay constraints, a bound on the cell-loss probability and other quality of service parameters. If the network can meet these requirements, a connection is made. Bursty traffic producing peak traffic rates in excess of the projected average rate could result in congestion and lead to performance degradation. As a result, the network might no longer be able to deliver the negotiated quality of service to existing connections. To lessen the chance of congestion, input rate control must be implemented.
This paper contains a performance analysis of the sticky buffer (SB), a moving window input rate control scheme intended to limit the rate at which input traffic may enter a network. Policing is achieved by buffering and hence delaying the entry of ATM-cells into the network. The scheme is determined by parameters (R,T) which specify that no more than R ATM-cells are permitted to enter the network in every window of size T cells. We present an exact analysis, deriving the probability generating function of the queue length distribution. A comparison with the leaky bucket is given. Our numerical examples show the required buffer size is comparable to that required by the leaky bucket. 相似文献