HDAC8-Selective Inhibition by PCI-34051 Enhances the Anticancer Effects of ACY-241 in Ovarian Cancer Cells

HDAC6 is overexpressed in ovarian cancer and is known to be correlated with tumorigenesis. Accordingly, ACY-241, a selective HDAC6 inhibitor, is currently under clinical trial and has been tested in combination with various drugs. HDAC8, another member of the HDAC family, has recently gained attention as a novel target for cancer therapy. Here, we evaluated the synergistic anticancer effects of PCI-34051 and ACY-241 in ovarian cancer. Among various ovarian cancer cells, PCI-34051 effectively suppresses cell proliferation in wild-type p53 ovarian cancer cells compared with mutant p53 ovarian cancer cells. In ovarian cancer cells harboring wild-type p53, PCI-34051 in combination with ACY-241 synergistically represses cell proliferation, enhances apoptosis, and suppresses cell migration. The expression of pro-apoptotic proteins is synergistically upregulated, whereas the expressions of anti-apoptotic proteins and metastasis-associated proteins are significantly downregulated in combination treatment. Furthermore, the level of acetyl-p53 at K381 is synergistically upregulated upon combination treatment. Overall, co-inhibition of HDAC6 and HDAC8 through selective inhibitors synergistically suppresses cancer cell proliferation and metastasis in p53 wild-type ovarian cancer cells. These results suggest a novel approach to treating ovarian cancer patients and the therapeutic potential in developing HDAC6/8 dual inhibitors.     

基于主成分的遗传神经网络股票指数预测研究

数据预测在金融投资领域占有重要地位,预测中输入变量的选取影响着预测的速度和精度,传统方法选取输入变量主观性较强,预测效果欠佳。将遗传算法与BP网络结合,利用GA的全局搜索优化BP网络的结构参数,有效克服BP算法的局部收敛等问题。使用主成分分析法选取输入变量,并将GA—BP混合建模应用于沪市综合指数预测中。实验结果表明,该方法改善了预测精度,达到了较好的预测效果。     

DHCPv6中的中继代理在IPv6路由器中的实现

随着IPv6下的动态主机配置协议DHCPv6的制定,各大设备和服务供应商已经开始支持DHCPv6的功能。本文对DHCPv6的特性、原理及工作过程做了介绍;设计实现了DHCPv6Relay功能,并详细介绍了中继代理对报文的处理过程。DHCPv6Relay已经在某公司的高端路由器SR88系列上投入了商用。     

在移动IP网络中实现Anycast服务的一种通信模型

提出了一种建立在移动IP网络中的Anycast通信模型,深入分析和讨论了该模型的可行性及其有效性,并论证此模型可以支持在移动IP网络中建设大规模的Anycast组。     

电加热方式在SF6开关设备中的应用

由于SF6气体在超低温下产生部分液化,使得SF6开关设备内的SF6气体密度下降,降低了SF6开关设备的开断能力和绝缘水平,无法保证SF6开关设备的可靠运行。为此,本文作者提出了采用电加热装置对SF6开关设备中的SF6气体进行加热,使SF6气体的温度可以始终高于SF6气体液化温度的解决方案。同时,本文对超低温环境下的其他解决方案也进行了分析比较。     

SF_6高压断路器状态检测技术介绍

介绍了高压断路器SF6气体压力(密度)、含水量的监测方法、高压断路器内绝缘的监测、导电回路的连接状态监测以及高压断路器机械性能测试手段,总结了目前高压断路器在相关参数、性能测试方面存在的不足,展望了在线监测技术的发展趋势.     

CKD6D型机车转向架的设计

简要介绍了CKD6D型机车转向架的总体布置、主要技术参数和结构特点。     

The m6A Methyltransferase METTL3-Mediated N6-Methyladenosine Modification of DEK mRNA to Promote Gastric Cancer Cell Growth and Metastasis

Gastric cancer (GC) is the fifth most common cancer and the third deadliest cancer in the world, and the occurrence and development of GC are influenced by epigenetics. Methyltransferase-like 3 (METTL3) is a prominent RNA n6-adenosine methyltransferase (m6A) that plays an important role in tumor growth by controlling the work of RNA. This study aimed to reveal the biological function and molecular mechanism of METTL3 in GC. The expression level of METTL3 in GC tissues and cells was detected by qPCR, Western blot and immunohistochemistry, and the expression level and prognosis of METTL3 were predicted in public databases. CCK-8, colony formation, transwell and wound healing assays were used to study the effect of METTL3 on GC cell proliferation and migration. In addition, the enrichment effect of METTL3 on DEK mRNA was detected by the RIP experiment, the m6A modification effect of METTL3 on DEK was verified by the MeRIP experiment and the mRNA half-life of DEK when METTL3 was overexpressed was detected. The dot blot assay detects m6A modification at the mRNA level. The effect of METTL3 on cell migration ability in vivo was examined by tail vein injection of luciferase-labeled cells. The experimental results showed that METTL3 was highly expressed in GC tissues and cells, and the high expression of METTL3 was associated with a poor prognosis. In addition, the m6A modification level of mRNA was higher in GC tissues and GC cell lines. Overexpression of METTL3 in MGC80-3 cells and AGS promoted cell proliferation and migration, while the knockdown of METTL3 inhibited cell proliferation and migration. The results of in vitro rescue experiments showed that the knockdown of DEK reversed the promoting effects of METTL3 on cell proliferation and migration. In vivo experiments showed that the knockdown of DEK reversed the increase in lung metastases caused by the overexpression of METTL3 in mice. Mechanistically, the results of the RIP experiment showed that METTL3 could enrich DEK mRNA, and the results of the MePIP and RNA half-life experiments indicated that METTL3 binds to the 3’UTR of DEK, participates in the m6A modification of DEK and promotes the stability of DEK mRNA. Ultimately, we concluded that METTL3 promotes GC cell proliferation and migration by stabilizing DEK mRNA expression. Therefore, METTL3 is a potential biomarker for GC prognosis and a therapeutic target.     

Relative Frequencies of PAX6 Mutational Events in a Russian Cohort of Aniridia Patients in Comparison with the World’s Population and the Human Genome

Genome-wide sequencing metadata allows researchers to infer bias in the relative frequencies of mutational events and to predict putative mutagenic models. In addition, much less data could be useful in the evaluation of the mutational frequency spectrum and the prevalent local mutagenic process. Here we analyzed the PAX6 gene locus for mutational spectra obtained in our own and previous studies and compared them with data on other genes as well as the whole human genome. MLPA and Sanger sequencing were used for mutation searching in a cohort of 199 index patients from Russia with aniridia and aniridia-related phenotypes. The relative frequencies of different categories of PAX6 mutations were consistent with those previously reported by other researchers. The ratio between substitutions, small indels, and chromosome deletions in the 11p13 locus was within the interval previously published for 20 disease associated genomic loci, but corresponded to a higher end due to very high frequencies of small indels and chromosome deletions. The ratio between substitutions, small indels, and chromosome deletions for disease associated genes, including the PAX6 gene as well as the share of PAX6 missense mutations, differed considerably from those typical for the whole genome.