Spectral and complexity analysis of scalp EEG characteristics for mild cognitive impairment and early Alzheimer's disease

Amnestic mild cognitive impairment (aMCI) often is an early stage of Alzheimer's disease (AD). MCI is characterized by cognitive decline departing from normal cognitive aging but that does not significantly interfere with daily activities. This study explores the potential of scalp EEG for early detection of alterations from cognitively normal status of older adults signifying MCI and AD. Resting 32-channel EEG records from 48 age-matched participants (mean age 75.7 years)—15 normal controls (NC), 16 early MCI, and 17 early stage AD—are examined. Regional spectral and complexity features are computed and used in a support vector machine model to discriminate between groups. Analyses based on three-way classifications demonstrate overall discrimination accuracies of 83.3%, 85.4%, and 79.2% for resting eyes open, counting eyes closed, and resting eyes closed protocols, respectively. These results demonstrate the great promise for scalp EEG spectral and complexity features as noninvasive biomarkers for detection of MCI and early AD.     

Automatic classification and prediction models for early Parkinson’s disease diagnosis from SPECT imaging

Early and accurate diagnosis of Parkinson’s disease (PD) is important for early management, proper prognostication and for initiating neuroprotective therapies once they become available. Recent neuroimaging techniques such as dopaminergic imaging using single photon emission computed tomography (SPECT) with ¹²³I-Ioflupane (DaTSCAN) have shown to detect even early stages of the disease. In this paper, we use the striatal binding ratio (SBR) values that are calculated from the ¹²³I-Ioflupane SPECT scans (as obtained from the Parkinson’s progression markers initiative (PPMI) database) for developing automatic classification and prediction/prognostic models for early PD. We used support vector machine (SVM) and logistic regression in the model building process. We observe that the SVM classifier with RBF kernel produced a high accuracy of more than 96% in classifying subjects into early PD and healthy normal; and the logistic model for estimating the risk of PD also produced high degree of fitting with statistical significance indicating its usefulness in PD risk estimation. Hence, we infer that such models have the potential to aid the clinicians in the PD diagnostic process.     

传染性法氏囊病病毒疫苗B87株基因组B节段的克隆和序列分析

目的获得传染性法氏囊病病毒(IBDV)疫苗B87株B节段全长cDNA,并对其序列进行分析,为进一步在分子水平上研究病毒基因组的功能、抗原变异和毒力变异奠定基础。方法使用蛋白酶K和酚/氯仿抽提法提取病毒基因组RNA,用LiCl分级沉淀方法纯化病毒基因组dsRNA,通过RT-PCR一步扩增获得B节段的全长cDNA。将其克隆到pGEM-T载体上,然后测序,并用DNAStar软件进行序列分析。结果测序结果表明,克隆的B87株基因组B节段全长为2827bp,与超强毒参考株UK661和HK46的同源性分别为89.8%和89.3%,与强毒参考株Harbin-1和ZJ2000的同源性分别为90.2%和89.5%;与变异毒株GLS的同源性为97.8%;与弱毒参考株CEF94和Gt株的同源性均为99.8%,与P2株的同源性达100%。结论通过对9株IBDV编码氨基酸序列进行分析、比较,推测B节段上10个独特的氨基酸位点可能与毒力相关。     

六种药剂防治向日葵白锈病试验研究

六种药剂防治向日葵白锈病田间药效试验结果表明,25%甲霜灵WP1000倍、50%安克WG2500倍的防效分别为87.6%和86.8%,显著优于64%杀毒矾WP800倍(防效75.7%),而52.5%抑快净WG1000倍、72%霜疫必克WP600倍和80%乙磷铝WP600倍的防效相当,不理想,均未达到50%。经方差分析证明处理间差异达到显著水平。     

马立克病病毒中meq基因对CEF细胞chTERT mRNA转录水平的影响

目的研究鸡马立克病病毒(MDV)meq基因对鸡胚成纤维细胞(CEF)的端粒酶催化亚单位基因(chTERT) mRNA转录水平的影响,并探讨meq基因与端粒酶活性的关系。方法构建重组载体pcDNA-meq,转染CEF细胞,利用Taq- Man探针,经实时荧光定量RT-PCR检测meq基因对CEF细胞chTERT mRNA转录水平的影响,并用TRAP法测定端粒酶活性。结果meq基因能激活CEF细胞中chTERT mRNA的转录,在48h的转录水平是72h的16倍,端粒酶活性在转染后48h是转染后72h的12倍。结论meq基因可能是MDV基因组中的主要致瘤基因,可以在体外导入正常的CEF细胞中,激活端粒酶的活性。     

植物病害图像压缩与传输的研究

为实现植物病害监控,研究了基于DSP的图像压缩编码,构建植物病害监测系统．系统通过CCD进行视频信息的采集,基于TMS320DM642DSP多媒体处理芯片采用H．263编码技术进行压缩编码,并将压缩后的数据经以太网传输至控制中心,进行图像识别分析,从而实现病害监控．测试表明,系统可成功进行图像的采集、压缩编码,并在PC端接收到压缩的图像．     

装配式预应力空心板桥面混凝土铺装层病害及防治

针对影响桥梁使用和维修保养的桥面铺装层问题,研究分析了装配式预应力混凝土空心板桥面混凝土铺装层的纵桥向裂缝、横桥向裂缝、龟裂、松散剥落及伸缩缝处破坏等常见病害的产生机理,提出了相应的设计与施工措施.概括总结了钢纤维混凝土、合成纤维混凝土、混杂纤维混凝土和钢纤维轻混凝土在桥面铺装层中的研究成果,列举了几个需要进一步深入研究的课题.     

危险传染病应急诊疗病房通风管道的优化设计

针对危险传染病的应急诊疗,研制出移动式危险传染病应急诊疗病房,为降低危险传染病应急诊疗病房的整体质量及棚顶的压力,以通风口的质量最轻为目标函数,以各个进气支路的进气量相等为约束条件,结合沿程阻力损失系数与管路横截面边长的关系式推导出通风管道约束函数关系式,利用MATLAB优化工具箱对模型求解,优化得到通风管道的最佳尺寸.根据优化设计结果设计了通风管道,满足实际使用要求.     

具有隔离接种且传染率为非线性的传染病模型的稳定性

研究一类具有预防接种、隔离且传染率依赖于易感人口的SIQRS传染病模型,给出确定疾病消亡和持续生存的基本再生数σ．在一定条件下证明无病平衡点的全局稳定性,得到唯一地方病平衡点的存在性和局部渐近稳定条件．     

作物病害胁迫检测的研究进展

综述了基于遥感技术、图像处理技术和农业专家系统的作物病害胁迫检测方法,比较这三种方法研究进展及其优缺点,介绍了近场声全息技术研究现状,提出了基于近场声全息理论的作物病害胁迫检测的研究方法,为作物病害综合精准防治开辟了一条新的途径．