Binding Properties of RNA Quadruplex of SARS-CoV-2 to Berberine Compared to Telomeric DNA Quadruplex

Previous studies suggest that berberine, an isoquinoline alkaloid, has antiviral potential and is a possible therapeutic candidate against SARS-CoV-2. The molecular underpinnings of its action are still unknown. Potential targets include quadruplexes (G4Q) in the viral genome as they play a key role in modulating the biological activity of viruses. While several DNA-G4Q structures and their binding properties have been elucidated, RNA-G4Qs such as RG-1 of the N-gene of SARS-CoV-2 are less explored. Using biophysical techniques, the berberine binding thermodynamics and the associated conformational and hydration changes of RG-1 could be characterized and compared with human telomeric DNA-G4Q 22AG. Berberine can interact with both quadruplexes. Substantial changes were observed in the interaction of berberine with 22AG and RG-1, which adopt different topologies that can also change upon ligand binding. The strength of interaction and the thermodynamic signatures were found to dependent not only on the initial conformation of the quadruplex, but also on the type of salt present in solution. Since berberine has shown promise as a G-quadruplex stabilizer that can modulate viral gene expression, this study may also contribute to the development of optimized ligands that can discriminate between binding to DNA and RNA G-quadruplexes.     

不同地应力下的地下硐室动态特征分析

采用FLAC3D对地震动荷载作用下不同地应力状态的地下岩体硐室的动态特性进行了初步分析。分析结果表明,地震荷载作用下,硐室位移随地应力侧压系数的增加明显减小,地应力侧压系数λ<1时的硐室位移量值随侧压系数的变化幅度明显大于地应力侧压系数λ>1时的情况;当侧压系数λ<1,随剪切波入射角的变化,硐室测量点位移响应先增加后减小;而当侧压系数λ>1,随剪切波入射角的增加,硐室测点位移先减小后增加,同时随地应力侧压系数的增加有减小的趋势。     

液压推移排矸架头的维护及改进

在总结近几年工作的基础上,对液压推移排矸架头的日常维护和保养作了分析总结,着重阐述其在实际应用过程中的不足之处,并有针对性地提出了进行改进的方法和建议,具有较强的实用价值和一定的借鉴意义。     

基础振动下直动式减压阀动态特性分析

针对基础振动对TBM液压元件性能的影响,为TBM液压元件优化设计提供理论依据,选择直动式减压阀为研究对象,分析其工作原理,建立减压阀的动态响应数学模型,仿真研究基础振动幅值和频率对减压阀波动特性的影响规律,分析减压阀不同结构参数对压力波动的影响.结果表明：基础振动会引起减压阀出口压力波动,波动幅值随振动幅值增加而增大;当基础振动频率大于50 Hz时,压力波动幅值随频率的增加明显增大;减小背压腔初始容积和回流通道直径能提高减压阀的动态稳定性.     

Creation of Nonspherical Microparticles through Osmosis‐Driven Arrested Coalescence of Microfluidic Emulsions

Droplet‐based microfluidics enable the production of emulsions and microparticles with spherical shapes, but the high‐throughput fabrication of nonspherical emulsions and microparticles still remains challenging because interfacial tension plays a dominant role during preparation. Herein, ionic liquids (ILs) containing salts, which possess sufficient osmotic pressure to realize water transport and phase separation, are introduced as inner cores of oil‐in‐oil‐in‐water double emulsions and it is shown that nonspherical emulsions can be constructed by osmosis‐driven arrested coalescence of inner cores. Subsequently, ultraviolet polymerization of the nonspherical emulsions leads to nonspherical microparticles. By tailoring the number, composition, and size of inner cores as well as coalescence time, a variety of nonspherical shapes such as dumbbell, rod, spindle, snowman, tumbler, three‐pointed star, triangle, and scalene triangle are created. Importantly, benefitting from excellent solvency of ILs, this system can serve as a general platform to produce nonspherical microparticles made from different materials. Moreover, by controlling the osmotic pressure, programmed coalescence of inner cores in double emulsions is realizable, which indicates the potential to build microreactors. Thus, a simple and high‐throughput strategy to create nonspherical microparticles with arrested coalescence shapes is developed for the first time and can be further used to construct novel materials and microreactors.     

MgO－CoO－NiO氧敏材料的缺陷化学及电导特性研究

本文用点缺陷理论分析了ＭｇＯ－ＣｏＯ－ＮｉＯ系氧敏材料的缺陷模型．并测量了ＭｇＯ－ＣｏＯ－ＮｉＯ材料的高温电导与氧分压的关系．在实验的基础上，研究了该材料的缺陷化学及电导特性，对其禁带宽度及缺陷的热力学参数进行了理论估算．实验结果与理论分析吻合得较好．     

煤巷锚杆支护的矿压监测

随着锚杆支护技术的不断推广应用,人们已经认识到矿压监测的重要性和必要性。因此对锚杆支护的矿区进行比较全面、系统的监测,并使监测工作走向正规化、日常化已势在必行。文章对矿压监测的方法及注意事项进行了论述。     

新型液压绳轮高位翻卸装车机的研发

新型液压绳轮高位翻卸装车机可实现高位翻卸矿车和高位装车2种功能,系统采用液压驱动,电气和液压联合控制,电气部分采用PLC集中编程全自动控制,节省人力、工人工作环境好、生产效率高。经过在龙口矿业集团北皂煤矿运行1 a验证,运行情况良好,完全达到了设计要求。     

门站调压系统自动识别选路装置的设计

介绍了天然气门站调压系统中自动识别选路装置的设计原则,探讨了自动识别选路装置的结构及控制过程。当运行路超压切断或不能满足下游用气时,备用路能自动启动供气。当运行路超压时,运行路紧急切断阀关闭,而备用路紧急切断阀不动作。     

电站锅炉压力容器检验单位资质问题的现状及思考

文章回顾了电力行业锅监工作的历史及检验体系的建立,针对目前电站锅炉压力容器检验单位资质问题的现状,为了保证对电力安全生产及锅炉压力容器设备的安全稳定运行,对检验单位的资质问题进行了分析,并对目前以及将来的检验工作、检验单位的发展、检验市场的建立等提出了建设性的意见。