基于AHP/QFD/FBS模型的果园管理机创新设计研究

目的 改善本土农机装备现行设计流程在方案创新中的不足,实现果园管理机要素的优化和设计的创新。方法 面向农业4.0场景应用,引入工业设计创新思维到农机装备设计,构建层次分析法(AHP)、质量功能展开(QFD)和FBS(Function-Behavior-Structure)模型相结合的创新设计流程。首先,通过AHP和QFD的分析过程,将用户需求转化为关键产品特性。然后,结合相关特性指标,优化、更新、重组产品功能的内在属性,逐步建立概念性功能系统。最后,通过FBS模型的“功能-行为-结构”映射,求解功能实现的结构载体,以满足产品功能创新和结构要素为设计条件。最终完成果园管理机创新设计。结果 集成AHP/QFD/FBS的创新设计流程包含完整的需求分析、特性发掘、功能创新和结构求解过程。通过设计实践,果园管理机在用户体验、功能配置、适应性和智能化方面得到了提升。结论 该流程在农机装备方案创新中呈现出的有效性,能够为农业4.0中相关产品设计提供新思路和参考。     

基于DFAD-FBS的自适应前臂骨折外固定护具设计

目的 针对前臂骨折外固定护具实时贴合性差的问题,进行自适应前臂骨折外固定护具产品设计研究。方法 将自适应设计方法（DFAD）与功能-行为-结构模型（FBS）相结合,构建自适应前臂骨折外固定护具设计方法,并基于形状记忆聚合物的仿生设计研究,进行自适应前臂骨折外固定护具产品设计。结果 基于DFAD-FBS设计方法对前臂骨折外固定护具进行分析,将仿生蜂窝结构的形状记忆聚合物应用于自适应前臂骨折外固定护具产品设计,并通过有限元分析和贴合度试验验证了产品设计的可行性。结论 DFAD-FBS设计方法为解决前臂骨折外固定护具的实时贴合问题提供了新的方向,并验证了形状记忆聚合物用于解决前臂骨折外固定护具贴合性的可行性,对产品的适应性设计问题提供了有意义的参考。     

基于FBS-TRIZ的园艺拖拉机设计研究

目的 关注园艺拖拉机使用过程中驾驶者的相关行为,提取园艺拖拉机驾驶者的使用需求,并将其转化为产品技术特性的设计要求,提升园艺拖拉机驾驶者的用户需求满意度。方法 将FBS-TRIZ理论融合作为研究园艺拖拉机功能需求映射的方法。通过对用户行为特征的分析,结合用户访谈、问卷调研等方式获取用户需求,运用FBS模型,构建出园艺拖拉机功能需求模型,将行为作为连接功能需求与产品结构的纽带,通过分析获取解决设计问题的方法;运用TRIZ理论进行冲突问题的分析与转化,并针对设计层面产品特性的矛盾提出解决方案。结论 FBS与TRIZ理论的融合应用能够将用户需求精准转化为技术特性层面的设计要求,对园艺拖拉机用户需求设计方面的不足提出了相应的解决方案,为设计师提供了将用户需求向功能结构的映射转化的指导,验证了该方法的有效性。     

Lyophilized Tea Extracts of Brickellia cavanillesii (Asteraceae): In Vitro Characterization of Biological Activity

Lyophilized Brickellia cavanillesii (LBC) tea extracts and identified chemical compounds of LBC were examined using in vitro human carcinoma liver (HepG2) cells with and without fetal bovine serum (FBS). Cells were incubated for 24 h with varying concentrations of FBS and LBC, respectively; cytotoxicity was determined spectrophotometrically using MTT (Formazan 3‐(4,5‐dimethyl‐thiazol‐2‐yl)‐2, 5‐diphenyl‐tetrazolium bromide) assay. Furthermore, the potential hypoglycemic activity of LBC tea extracts was investigated using glucose transport and metabolism proteins biomarkers. FBS (0% to 10%) increased the viability of HepG2 cells steadily with increasing concentration. Possible therapeutic effects of LBC were concentration dependent with and without FBS. The cytotoxicity of 12 identified compounds from the LBC extract suggests that the individual compounds inhibited the proliferation of HepG2 cells differentially and do not reflect the inhibition of the whole aqueous LBC. Western blot analysis of glucose facilitated transporter protein 2 (GLUT 2) expression of HepG2 cells exposed to 0 mg/mL (Control) and 0.2 mg/mL LBC for 2, 4, 6, and 24 h suggests that GLUT 2 expression was increased. Increase in GLUT 2 expression in the absence of FBS was statistically significant with time of exposure. Significant difference was observed for GLUT 2 expression between 6 and 24 h and also between 4 and 24 h at 0.2 mg/mL LBC. Results obtained indicate that LBC may exhibit antidiabetic activity. However, further studies will be necessary to clearly delineate LBC potential therapeutic benefit and biological activities in animal studies as well as other in vitro models.     

快速适应电网结构变化的广域后备保护新方法

提出一种能够快速适应电网变结构的广域后备保护新方法。该方法在电力系统正常运行的情况下,首先利用故障等值模型计算故障点的等值注入电流,再根据网络拓扑参数和节点注入电流形成支路相关因子矩阵,确定故障界定集;在网络拓扑发生变化后,通过修改支路相关因子矩阵中部分元素,实现故障界定集的快速更新。当电力系统出现故障时,迅速计算故障界定集与支路电流测量值之间贴近度的最小值,并以此确定故障位置。新英格兰10机39节点系统仿真结果表明,该方法不受系统运行方式的影响,能够在有限测点及网络变结构情况下准确、灵敏、可靠地实现故障定位。     

Expression of the C-Terminal Domain of Phospholipase Cβ3 Inhibits Signaling via Gαq-Coupled Receptors and Transient Receptor Potential Channels

Transient receptor potential (TRP) channels are cation channels that play a regulatory role in pain and thermosensation, insulin secretion, and neurotransmission. It has been proposed that activation of TRP channels requires phosphatidylinositol 4,5-bisphosphate, the major substrate for phospholipase C (PLC). We investigated whether inhibition of PLCβ has an impact on TRP channel signaling. A genetic approach was used to avoid off-target effects observed when using a pharmacological PLCβ inhibitor. In this study, we show that expression of PLCβ1ct and PLCβ3ct, truncated forms of PLCβ1 or PLCβ3 that contain the C-terminal membrane binding domains, almost completely blocked the signal transduction of a Gαq-coupled designer receptor, including the phosphorylation of ERK1/2. In contrast, expression of the helix-turn-helix motif (Hα1—Hα2) of the proximal C-terminal domain of PLCβ3 did not affect Gαq-coupled receptor signaling. PLCβ3ct expression impaired signaling of the TRP channels TRPM3 and TRPM8, stimulated with either prognenolone sulfate or icilin. Thus, the C-terminal domain of PLCβ3 interacts with plasma membrane targets, most likely phosphatidylinositol 4,5-bisphosphate, and in this way blocks the biological activation of TRPM3 and TRPM8, which require interaction with this phospholipid. PLCβ thus regulates TRPM3 and TRPM8 channels by masking phosphatidylinositol 4,5-bisphosphate with its C-terminal domain.     

基于FBS的注塑模抽芯机构概念设计方法研究

针对注塑模抽芯机构设计的特点与要求，应用基于功能一行为一结构的概念设计方法，通过分析注塑模机构概念设计的一般过程，建立了基于FBS的注塑模抽芯机构分层映射模型和求解模型，实现了抽芯机构概念设计的智能化。     

Inhibitory effect of aqueous spinach extract on degranulation of RBL-2H3 cells

The inhibitory effect of an aqueous extract from spinach on degranulation of RBL-2H3 cells is herein reported. The extract significantly suppressed antigen-induced degranulation in a dose-dependent manner without affecting cell viability. Active substances in the extract were heat-stable and trypsin-resistant with molecular weights ranging from 500 Da to 14 kDa. The extract inhibited elevation of the intracellular Ca²⁺ concentration caused by stimulation by antigen, while not suppressing degranulation induced by a calcium ionophore A23187. Immunoblot analysis revealed that the inhibitory effect results from downregulation of phosphorylation of both Syk kinase and phosphatidylinositol 3-kinase in the signalling pathways involved in degranulation caused by the antigen–antibody interaction. Taken together, these findings suggest that aqueous spinach extract has an anti-allergic activity that controls degranulation.     

Optimization of cryopreservation procedures for porcine endothelial progenitor cells

Endothelial progenitor cells (EPCs) provide a powerful option for therapeutic use in ischemic diseases. The cell therapy-induced vasculogenesis requires sufficient homogeneous cells, and cryopreservation is a prerequisite for long-term storage and quality assurance of EPCs. The aim of this study was to optimize cryopreservation protocols of EPCs derived from porcine bone marrow. Bone marrow-derived mononuclear cells (MNCs) were isolated by density centrifugation and differentiated into EPCs. The first passage EPCs were frozen by using different methodologies, and after cryopreservation the thawed cells were cultured to the fourth passage. The recovery efficiency and functions of these cells were evaluated by determination of cell viability, proliferation and migration. We found the optimal conditions for cryopreservation of EPCs as follows: (i) a cryopreservation medium consisting of 10% dimethylsulphoxide (DMSO) in combination with 50% fetal bovine serum (FBS); (ii) using a controlled freezing rate at 5°C/min; (iii) at an optimal density of 5 × 10⁶/ml for cryopreserved EPCs; (iv) a storage temperature of − 156°C. Under these conditions we demonstrated that EPCs could be stored in mechanical freezer for up to 18 months after cryopreservation without losing their phenotypic characteristics and biological functions.     

Protection of lung fibroblast MRC-5 cells against hydrogen peroxide-induced oxidative damage by 0.1-2.8 kDa antioxidative peptides isolated from whey protein hydrolysate

Antioxidative peptides (0.1-2.8 kDa) obtained from gel filtration of Alcalase-hydrolysed whey protein were subjected to individual peptide isolation by RP-HPLC. The sub-fraction P4 and a prominent pentapeptide identified by mass spectrometry (Val-His-Leu-Lys-Pro) (P4c) were found to be highly antioxidative, therefore, used to assess the efficacy against hydrogen peroxide (H(2)O(2))-induced human lung fibroblast MRC-5 cell oxidative injury. MRC-5 cells were damaged by incubation with H(2)O(2), but cell death was significantly reduced when exposed to P4 and P4c (P<0.05). Compared to the H(2)O(2)-damaged model control, P4 and P4c enhanced the activity of cell superoxide dismutase, glutathione peroxidase, and catalase, and decreased the levels of malonaldehyde (P<0.05). These results, with a more pronounced action of P4c than P4, demonstrated that antioxidative peptides had a significant protection of MRC-5 cells against the toxicity caused by H(2)O(2).