用面向对象编程实现问题求解自动化

本文从面向对象方法基本原理和面向对象编程的技术特征出发，阐明所建应用软件系统中的对象，类及相应类层次结构和类组合结构，使系统具有很强的表现真空世界复杂系统结构的能力，系统通过消息传递在程序执行中实现对操作的调用机制，使之在面向用户问题选择和执行求解策略方面有很强的适应性，这一点对于强调人－机交互和解题协作的智能决策支持系统设计至关重要，文章给出了用Ｃ＋＋实现的模型对象系统及消息传递机制。     

RFID复杂事件处理关键技术的研究与改进

RFID复杂事件处理是一个新兴的技术领域,它用来处理大量的简单事件,并从中整理出有价值的事件。RFID事件和传统的事件相比较具有海量性、空间性和时间性、数据不准确性等特征。文中在分析RFID数据特点的基础上,对RFID复杂事件处理的关键技术进行研究和改进,主要介绍RFID数据的清洗和事件检测技术。对于RFID数据清洗部分,提出了多层次过滤的方法使得到的数据更接近真实情况,而事件检测方面则提出了局部检测和全局检测相结合的方法对相关数据进行检测以得到更有意义的数据供上层应用使用。最后,对RFID复杂事件处理的发展趋势做出展望。     

Stability analysis and H∞ control for hybrid complex dynamical networks with coupling delays

This paper formulates and studies a model of complex dynamical networks with switching topology and coupling delays. Based on the hybrid control and Lyapunov function, the stability and robust H_∞ control of such networks with impulsive and switching effects, which have not been studied before, are addressed with some criteria derived. Examples are given to illustrate the effectiveness of the theoretical results. Copyright © 2011 John Wiley & Sons, Ltd.     

工业设计决策网络构建及其动态演化仿真

针对工业设计过程多阶段方案决策的意见演化问题,融合复杂网络理论建立工业设计决策网络模型,通过意见动力学进行决策意见动态演化仿真.基于图论建立设计决策有权无向网络,以网络效率变化确定决策者权重,借助意见距离识别设计决策网络节点信任集合,利用加权平均意见驱动设计决策网络更新与演化. 提出工业设计决策网络的演化仿真流程,结合产品设计方案决策数据进行动态仿真分析,结果表明：信任阈值决定设计决策网络拓扑构成,随着网络演化的进行,决策个体间意见差异逐步缩小并最终达成共识;设计决策网络分析能够析出噪声节点及其意见变化,在实际产品设计决策中应重点关注;意见演化能够辅助确定工业设计方案决策轮次,明确方案在各指标上的表现并识别设计改进方向;仿真分析有助于发现设计决策中的关键因素和意见演化规律.     

Nickel-Carnosine complex:A new carrier for enzymes immobilization by affinity adsorption

Immobilization is an effective method to promote the application of enzyme industry for improving the stability and realizing recovery of enzyme.To some extent,the performance of immobilized enzyme depends on the choice of carrier material.Therefore,the development of new carrier materials has been one of the key issues concerned by enzyme immobilization researchers.In this work,a novel organic-inorganic hybrid material,nickel-carnosine complex (NiCar),was synthesized for the first time by solvothermal method.The obtained NiCar exhibits spherical morphology,hierarchical porosity and abun-dant unsaturated coordination nickel ions,which provide excellent anchoring sites for the immobiliza-tion of proteins.His-tagged organophosphate-degrading enzyme (OpdA) and ω-transaminase (ω-TA)were used as model enzymes to evaluate the performance of NiCar as a carrier.By a simple adsorption process,the enzyme molecules can be fixed on the particles of NiCar,and the stability and reusability are significantly improved.The analysis of protein adsorption on NiCar verified that the affinity adsorp-tion between the imidazole functional group on the protein and the unsaturated coordination nickel ions on NiCar was the main force in the immobilization process,which provided an idea way for the develop-ment of new enzyme immobilization carriers.     

Structural Investigation and Molecular Modeling Studies of Strobilurin-Based Fungicides Active against the Rice Blast Pathogen Pyricularia oryzae

The increasing emergence of fungicide-resistant pathogens requires urgent solutions for crop disease management. Here, we describe a structural investigation of new fungicides obtained by combining strobilurin and succinate dehydrogenase inhibitor pharmacophores. We identified compounds endowed with very good activity against wild-type Pyricularia oryzae, combined in some cases with promising activity against strobilurin-resistant strains. The first three-dimensional model of P. oryzae cytochrome bc1 complex containing azoxystrobin as a ligand was developed. The model was validated with a set of commercially available strobilurins, and it well explains both the resistance mechanism to strobilurins mediated by the mutation G143A and the activity of metyltetraprole against strobilurin-resistant strains. The obtained results shed light on the key recognition determinants of strobilurin-like derivatives in the cytochrome bc1 active site and will guide the further rational design of new fungicides able to overcome resistance caused by G143A mutation in the rice blast pathogen.     

ATP-Independent Initiation during Cap-Independent Translation of m6A-Modified mRNA

The methylation of adenosine in the N⁶ position (m⁶A) is a widely used modification of eukaryotic mRNAs. Its importance for the regulation of mRNA translation was put forward recently, essentially due to the ability of methylated mRNA to be translated in conditions of inhibited cap-dependent translation initiation, e.g., under stress. However, the peculiarities of translation initiation on m⁶A-modified mRNAs are not fully known. In this study, we used toeprinting and translation in a cell-free system to confirm that m⁶A-modified mRNAs can be translated in conditions of suppressed cap-dependent translation. We show for the first time that m⁶A-modified mRNAs display not only decreased elongation, but also a lower efficiency of translation initiation. Additionally, we report relative resistance of m⁶A-mRNA translation initiation in the absence of ATP and inhibited eIF4A activity. Our novel findings indicate that the scanning of m⁶A-modified leader sequences is performed by a noncanonical mechanism.     

The Mystery of Extramitochondrial Proteins Lysine Succinylation

Lysine succinylation is a post-translational modification which alters protein function in both physiological and pathological processes. Mindful that it requires succinyl-CoA, a metabolite formed within the mitochondrial matrix that cannot permeate the inner mitochondrial membrane, the question arises as to how there can be succinylation of proteins outside mitochondria. The present mini-review examines pathways participating in peroxisomal fatty acid oxidation that lead to succinyl-CoA production, potentially supporting succinylation of extramitochondrial proteins. Furthermore, the influence of the mitochondrial status on cytosolic NAD⁺ availability affecting the activity of cytosolic SIRT5 iso1 and iso4—in turn regulating cytosolic protein lysine succinylations—is presented. Finally, the discovery that glia in the adult human brain lack subunits of both alpha-ketoglutarate dehydrogenase complex and succinate-CoA ligase—thus being unable to produce succinyl-CoA in the matrix—and yet exhibit robust pancellular lysine succinylation, is highlighted.     

高铁三水铝石型铝土矿的烧结特性

以Al2O3含量为26.35%,总Fe含量为31.22%,铝硅比为3.17的某高铁三水铝石型铝土矿为原料,研究了碱度、焦粉用量、混合料水分等对烧结矿质量指标的影响。结果表明:烧结矿碱度增加时,其转鼓强度和成品率下降,烧结矿自粉化程度加重;焦粉用量增加时,烧结矿转鼓强度和成品率提高;当混合料水分为9.0%、焦粉用量为8.0%、烧结矿碱度为4时,烧结矿转鼓强度为69.1%,成品率为81.2%,垂直烧结速度为30 mm/min,利用系数为1.2 t/(h.m2);烧结矿碱度为4时,样品存放2天粉化率为5%,烧结矿平均粒度由22.2 mm减小到21.9 mm,延长存放时间,烧结矿自粉化率增大,平均粒度显著减小。该烧结矿的冶金性能能基本满足高炉冶炼的要求。