植物花青素通过JNK信号通路抗肿瘤机制研究进展

文章综述了JNK信号通路的组成及活化机制,总结了植物花青素通过该信号通路抑制乳腺癌、前列腺癌、肝癌等恶性肿瘤发生、增殖、侵袭和凋亡的能力,以期为植物花青素应用于功能食品和药品的开发提供依据。     

Convergence of rule-of-thumb sizing and allocating rules of distributed generation in meshed power networks

This paper presents different approaches to find out and address some rules for distributed generation (DG) integrated mesh type networks, which can be used in the management of future power systems. There are so many influencing factors of efficiency in the integration of DG that we need to analyze these influencing factors obviously. Hence, carefully planning plays a key role in tackling these challenges in the future power systems. In contrast to the majority of existing observations, we focus on the case where the underlying states are multiple and single DG allocations with changing conditions. In several previous studies, the best single bus has been investigated under the specified conditions. However, it follows from the results of this study that all issues concerning DG strongly depend on power network structure and DG locations, and it is worth to note that the best location changes with penetration levels. Also, it is observed that the all buses show different characteristics in terms of DG integrations under the different cases, moreover their optimum size and power factor are different. It means that optimum bus in a network changes with the conditions. On the other hand, the problematic buses can be occurred in voltage profile after the DG integration. As a result, an investigation of rule of thumb approach is performed for evaluation of performance enhancement of DG integrated meshed networks. The results are also used to discuss the integration of DG management strategies under various operating conditions.     

Inhibitors of Acetylcholinesterase and Butyrylcholinesterase Meet Immunity

Acetylcholinesterase (AChE) inhibitors are widely used for the symptomatic treatment of Alzheimer’s disease and other dementias. More recent use is for myasthenia gravis. Many of these inhibitors interact with the second known cholinesterase, butyrylcholinesterase (BChE). Further, evidence shows that acetylcholine plays a role in suppression of cytokine release through a “cholinergic anti-inflammatory pathway” which raises questions about the role of these inhibitors in the immune system. This review covers research and discussion of the role of the inhibitors in modulating the immune response using as examples the commonly available drugs, donepezil, galantamine, huperzine, neostigmine and pyridostigmine. Major attention is given to the cholinergic anti-inflammatory pathway, a well-described link between the central nervous system and terminal effector cells in the immune system.     

天麻素联合地塞米松保护缺糖缺氧诱导的H9C2细胞损伤

目的：探讨天麻素（gastrodin, GAS）联合地塞米松（dexamethasone, DEX）在缺糖缺氧诱导心肌细胞损伤中的作用及可能机制。方法：建立缺糖缺氧细胞（oxygen-glucose deprivation, OGD）模型,细胞分为5组,即正常对照组（normal group）,缺糖缺氧组（OGD group）,DEX组,GAS组,DEX+GAS组。利用CCK-8实验检测各组心肌细胞活性,利用比色法检测乳酸脱氢酶（LDH）的活性;利用TUNEL法检测各组心肌细胞的凋亡情况;利用ELISA实验检测各组心肌细胞培养液中炎性因子;利用Western blot检测各组心肌细胞中Notch1、Bax、Bcl-2及Beclin1的表达情况。结果：结果显示GAS与DEX联合使用可显著提高损伤心肌细胞的活性,减少心肌细胞的凋亡;降低促炎因子TNF-α、IL-6和IL-1β的产生及促进抑炎因子IL-10的产生,降低LDH的释放;Western blot结果显示GAS与DEX联合使用可促进Notch信号通路中Notch1的表达,显著降低受损心肌细胞中促凋亡蛋白Bax的表达,促进抑凋亡蛋白Bcl-2的表达,促进自噬相关基因Beclin1的表达。 结论：GAS与DEX联合使用,可能通过促进Notch信号通路的激活,促进其自噬,提高细胞的活性,抑制心肌细胞的凋亡,减轻炎症反应,从而减轻OGD诱导的心肌细胞损伤。     

滑动弧放电等离子体-溶液系统协同零价铁降解酸性橙Ⅱ

为探究等离子体-溶液系统联合零价铁(ZVI)Fenton反应对酸性橙Ⅱ(AOⅡ)的降解效果,考察ZVI对降解过程的促进作用及降解机理,建立了滑动弧等离子-溶液系统进行了实验研究。以质量浓度200 mg/L的AOⅡ为例,改变ZVI投加量分别为0、4、7、10、13 g。结果显示ZVI投加量为10 g时,AOⅡ的降解程度最高,为97.8%。等离子体-溶液系统中滑动弧放电产生的H2O2与加入的ZVI结合发生Fenton反应,AOⅡ降解过程遵守一级动力学规律。对比不加入ZVI时的AOⅡ反应速率常数,加入10 g ZVI后初始质量浓度为100、150、200、250、300 mg/L的AOⅡ反应速率常数分别提高了12.9%、31.0%、39.4%、44.3%、47.8%。溶液化学需氧量(COD)去除率也表明了在降解过程中加入ZVI能够有效地提高降解率。运用色谱-质谱联用仪(GC-MS)测定了AOⅡ的降解机理,认为*OH攻击AOⅡ分子上与萘环相连的—N N—键,导致—N N—键的断裂,并经过一系列的氧化过程后形成无机小分子物质。     

国内运营商国际互联网互联互通方案探讨

随着海外互联网市场的不断拓展,中国电信、中国联通等运营商,通过自建海外IP网络、与国际运营商网络互联互通等方式不断扩大网络全球覆盖、提升网络可达范围、引入更丰富互联网资源。从国际互联网互联互通架构与模式等出发,基于国内运营商的国际互联网互联互通建设及运营过程中的互联方寻找、互联互通需求评估、模式选择、互联互通落地及优化、互联互通结算等方面视角,研究了国际互联网互联互通解决方案,并提供了可供参考的互联互通部署建议。     

联网风机盘管控制系统在绿色医院中的应用

基于物联网技术的中央空调风机盘管控制系统是未来绿色医院建设的必然选择,本文以EHS-7000系统为例,介绍了系统的设计、应用及软件的功能。     

重组人促红细胞生成素对人乳腺癌MDA-MB-231细胞增殖的影响及其作用机制研究

目的 探讨重组人促红细胞生成素（recombinant human erythropoietin,rh-EPO）对人乳腺癌MDA-MB-231细胞增殖的影响及其作用机制.方法 将人乳腺癌 MDA-MB-231 细胞进行培养.传至5～6代,细胞生长状态稳定后,收集人乳腺癌 MDA-MB-231细胞用于MTT实验.采用MTT法检测 5 组（阴性对照组、rh-EPO A 组、rh-EPO B组、rh-EPO C 组和rh-EPO D 组）MDA-MB-231细胞增殖的情况.用10 μmol·L-1p38MAPK抑制剂SB203580、ERK抑制剂U0126、JNK抑制剂SP600125和NF-κB 抑制剂PDTC预处理人乳腺癌 MDA-MB-231 细胞后,用MTT法检测经100、200、300和400 U·mL-1的rh-EPO（PDTC＋EPO 组、SB203580＋EPO 组、SP600125＋EPO组和U0126＋EPO组）诱导后细胞增殖的情况.结果 阴性对照组、rh-EPO A 组、rh-EPO B组、rh-EPO C 组和rh-EPO D 组 72 h PI值分别为：1.000 0±1.000 0、1.231 8±0.133 0、1.323 9±0.136 0、1.351 7±0.146 0和1.423 1±0.084 0;96 h PI值分别为：1.000 0±1.000 0、1.352 5±0.036 0、1.359 7±0.112 0、1.387 2±0.063 0和1.410 8±0.060 0.rh-EPO A 组、rh-EPO B组、rh-EPO C 组和rh-EPO D 组 72、96 h PI值与阴性对照组比较差异均有统计学意义（均P＜0.05）.PDTC＋EPO 组、SB203580＋EPO 组72、96 h PI值均较EPO组明显降低（均P＜0.05）,SP600125＋EPO组、U0126＋EPO组72、96 h PI值与EPO组比较差异均无统计学意义（均P＞0.05）.结论 rh-EPO可能是通过NF-κB、MAPK传导通路发挥效应,促进人乳腺癌MDA-MB-231细胞增殖.     

临床护理路径在脑出血患者护理中的应用

目的 探讨临床护理路径在脑出血患者中的应用价值.方法 选取120例脑出血患者为研究对象,将其按不同的护理方法分为2组.观察组（n=60）采用临床护理路径进行护理,对照组（n=60）采用传统的护理模式进行护理,观察2组患者对护理的满意度及相关临床指标.结果 观察组在技术质量安全、服务及时、健康教育、人文关怀、服务态度、出入院教育等方面满意度明显优于对照组（P＜0.01）;观察组患者住院时间、住院费用、并发症发生率均明显少于对照组（P＜0.01）;出院后3个月观察组FIM评分和Fugl-Meyer评分明显高于对照组（P＜0.01）.结论 临床护理路径在脑出血护理中是一种有效的护理模式,可以有效提高患者满意度和临床疗效.