Optimization of substrates for Bioelectricity production by Ochrobactrum pseudintermedium KF026284 in a single chambered microbial fuel cell

A new strain of bacterium Ochrobactrum pseudintermedium KF026284 was isolated from a single chambered microbial fuel cell operated with rumen fluid. The bacterium produced maximum power density of 114 mW/m² (0.7 V, 0.6 mA) when nutrient broth was used as the growth medium. The optimization of electricity generation by O. pseudintermedium KF026284 was carried out using various substrates like cellulose, cellobiose, starch, sucrose, and glucose. The bacterium when fed with cellobiose showed an appreciable and sustainable electricity generation with a power density of 150 mW/m² from the 5th day and a maximum power density of 247 mW/m² on the 11th day.     

Effect of hydrogen producing mixed culture on performance of microbial fuel cells

This study examined the influence of H₂-producing mixed cultures on improving power generation using air-cathode microbial fuel cells (MFCs) inoculated with heat-treated anaerobic sludge. The MFCs installed with graphite brush anode generated higher power than the MFCs with carbon cloth anode, regardless heat treatment of anaerobic sludge. When the graphite brush anode-MFCs were inoculated selectively with H₂-producing bacteria by heat treatment, power production was not improved (about 490 mW/m²) in batch mode operation, but for slightly increased in carbon cloth anode-MFCs (from 0.16 to 2.0 mW/m²). Although H⁺/H₂ produced from H₂-producing bacteria can contribute to the performance of MFCs, suspended biomass did not affect the power density or potential, but the Coulombic efficiency (CE) increased. A batch test shows that propionate and acetate were used effectively for electricity generation, whereas butyrate made a minor contribution. H₂-producing mixed cultures do not affect the improvement in power generation and seed sludge, regardless of the pretreatment, can be used directly for the MFC performance.     

Harvest of electrical energy from fermented microalgal residue using a microbial fuel cell

The application of microalgal biomass for fermentation has been highlighted as a means of producing a range of value-added biofuels and chemicals. On the other hand, the microalgal residue from the fermentation process still contains as much as 50% organic contaminants, which can be a valuable substrate for further bioenergy recovery. In this study, a microbial fuel cell and automatic external load control by maximum power point tracking (MPPT) were implemented to harvest the electrical energy from waste fermented microalgal residue (FMR). The MFC with MPPT produced the highest amount of energy (1.82 kJ/L) compared to the other MFCs with fixed resistances: 0.98 (1000 Ω), 1.16 (500 Ω), and 1.17 kJ/L (300 Ω). The MFC with MPPT also showed the highest maximum power density (88.6 mW/m²) and COD removal efficiency (620.0 mg COD/L removal with 85% removal efficiency). The implementation of MPPT gained an approximate 12.9% energy yield compared to the previous fermentation stage. These results suggest that FMR can be an appropriate feedstock for electrical energy recovery using MFCs, and the combined fermentation and MFC system improves significantly the energy recovery and treatment efficiency from FMR.     

微生物燃料电池(MFC)技术及其发展前景的研究

本文论述了能源和环境面临的问题与微生物燃料电池（MFC）的关系,介绍了MFC产能原理和效率及其国内外研究进展,分析了利用湿地微生物构建生物质能源循环式微生物电池的潜力,指出了MFC作为一种可再生的清洁新能源的广阔前景。     

Enhanced sludge stabilization coupled with microbial fuel cells (MFCs)

This study presents research results on electricity production from waste activated sludge using MFCs during stabilization process. Different MFC configurations equipped with various electrodes were used. Voltage measurements were continuously done during 35 days of MFC operation. Experimental results showed that bioelectricity generation was linked to volatile solids (VS) and protein reductions as a fraction of extracellular polymeric substances (EPS). Double chamber MFC reactor equipped with graphite electrodes had better power and current densities as 312.98 mW/m² and 39.07 μA/cm² while single chamber MFC equipped with titanium electrodes revealed better power and current densities as 97.60 mW/m² and 17.63 μA/cm², respectively. Molecular results indicated that power outputs of MFCs effected by diverse microbial communities in anode biofilms. Although organic matter degradation is reported as 35%–55% VS reduction for digesters, this research provided a promising approach for sludge stabilization with enhanced degrading of organic matters up to 75% by using MFCs.     

Synergistic interaction of biocatalyst with bio-anode as a function of electrode materials

Comprehensive study was performed to understand the synergistic interaction between the biocatalyst and anode in terms of electron discharge (ED) pattern and microbial growth by varying electrode (bio-anode) materials viz., graphite, aluminum, brass, copper, nickel and stainless steel. Experiments were performed in bio-electrochemical cell consisting of three electrodes (bio-anode as working electrode, carbon rod as counter electrode and Ag/AgCl(S) as reference electrode) employing anaerobic mixed culture as anodic biocatalyst. Voltammetric and chronoamperometric analysis were used to enumerate the ED and redox reactions. Presence of higher microbial population and dominance of Gram positive bacteria with higher ED supported graphite function as a good bio-anode material. Nickel and stainless steel showed higher ED after graphite associated with dominance of Gram positive bacterial population. Although higher ED was noticed with brass, metal oxidation and decrement in ED with time doesn’t support its function as bio-anode. In spite of higher ED than nickel and stainless steel, aluminum and copper showed significant metal oxidation leading to change in both physical and electrochemical properties along with dominant growth of Gram negative bacteria. This study gives a comprehensive idea on biocatalyst interaction with anode in extracellular electron transfer which is important in improving the anode performance. Juxtaposing the results, it can be deduced that the outcome of the present study can be extended to all bio-electrochemical systems including microbial fuel cell (MFC).     

Microbial fuel cell (MFC) using commercially available unglazed ceramic wares: Low-cost ceramic separators suitable for scale-up

This study aimed to evaluate the influence of commercially available unglazed wall ceramic (UGWC) and unglazed floor ceramic (UGFC) separators with different thickness and porosity on the performance of dual-chamber microbial fuel cells (MFCs). These MFCs were operated under continuous condition using domestic wastewater. The UGWC-based MFC produced higher maximum power density (321 mW/m² with a thickness of 9 mm) than UGFC-based MFC (106.89 mW/m² with a thickness of 3 mm) due to lower internal resistance. Power generation using both types of separators was lower than that of obtained using the Nafion 117 membrane as control (602 mW/m²). The maximum average coulombic efficiencies (CE) of the UGWC-based MFCs (with thickness levels of 6 and 9 mm) were 58% and 68%, respectively, which was more than that of UGFC-based MFCs and also control MFC (53%). Voltammetric analysis revealed that the maximum peak current of 6 mA was obtained for UGWC-based MFC which was in the order of control MFC (5.9 mA). The UGWC separators exhibited smaller ohmic and diffusion resistances of 57, 65 and 87 Ω in MFCs at the thickness levels of 3, 6 and 9 mm, respectively, compared to the UGFC separators with that of 164.27 and 366.23 Ω in MFCs at the thickness levels of 3 and 6 mm, respectively. UGWC separators because of their low production cost, high mechanical strength and increased output power density of the MFC proved to be a suitable alternative to replace with a costly polymeric membrane such as Nafion 117.     

Anodic potential on dual-chambered microbial fuel cell with sulphate reducing bacteria biofilm

Dual chamber microbial fuel cell reactors were inoculated with a mixed culture of sulfate-reducing bacteria with anode potential being the controlling parameter. A negative poised anode potential enhanced the performance of this fuel cell while a positive poised anode potential had adverse effects on cell performance. Negative anodic potential affects the biofilm characteristics, as evidenced by electrochemical analysis. Microbial community was changed accordingly.     

Factors that influence the performance of two-chamber microbial fuel cell

The two-chamber microbial fuel cell (MFC) was operated in batch mode, using acclimated hydrogen-producing mixed bacteria as the anodic inoculum, artificial sucrose wastewater as the substrate (sucrose concentration 10.0 g/L). The performance of the MFC was analyzed at different anodic pH microenvironments, such as the initial pH of the anolyte of 8.57, 7.3, 7.0 and 6.0, respectively, while anodic pH-controlled of 7.3 and 7.0. It showed that the best performance was obtained when the MFC was carried out at anodic pH-controlled of 7.3. Taking the interaction of factors into consideration, we adopted response surface methodology (RSM) to investigate the effects of sucrose concentration, operating temperature and ferrous sulfate concentration on the performance of MFC. The optimum condition for maximum output voltage of the two-chamber MFC (external resistance 1000 Ω) was thus obtained.     

基于 DirectShow的通用多路视频接口研究和实现

本文在多路视频监控系统背景下,主要研究和实现了基于DirectShow架构且能响应视频区域鼠标消息的通用多路视频接口,并成功地把该接口应用于该多路系统中。本文设计 的接口,不需要考虑视频捕获硬件的差异,可同时处理多路视频（播放、暂停、捕获和视频区域任意切分）,并接受用户直接在视频区域的鼠标操作,灵活控制各路视频。该
该接口提供一个函数和二个类,以MFC扩展DLL的形式封装。