基于LabVIEW的UHF RFID射频识别系统的设计

针对目前市场流行的超高频RFID识别系统成本高、制作方法复杂等问题,提出了一种基于射频读写专用芯片MagicRF M100的兼容国际标准ISO/IEC 18000-6C和EPC Class-1 Generation-2协议的UHF RFID读写器的设计方案,详细阐述了读写器的工作原理,并相应地介绍了系统软硬件设计中比较...     

Creation of Nanotips on ITO Electrode by Nanoparticle Deposition: An Easy Way to Enhance the Performance of Electrically Responsive Photonic Crystal and Fabricate Electrically Triggered Anticounterfeiting Tags

Electrically responsive photonic crystals (ERPC) are promising materials for electrophoretic displays, smart windows, and tunable optical devices. However, the high working voltage limits the application of most polar ERPCs because it damages color saturation, response stability, and reversibility. Herein, an easy but general method based on the modification of electrodes with nanoparticles is developed to improve ERPC's performance. The deposition of Au, Cu, or Cu₂O nanoparticles created nanotip structures on the electrode, which enhanced the charge injection and local electric field strength, lowered 43% of the working voltage, and increased the color saturation. Meanwhile, the lowered voltage inhibited the electrodeposition of colloidal particles and the electrochemical reaction, which also improved the stability and reversibility of the electrical response. Based on the region-selective modification of the electrode and the resulting asymmetric electrical response, an electrically triggered tag with a “pattern showing/hiding” effect is fabricated for anticounterfeiting purposes.     

基于树莓派的无接触智慧电梯设计

传统的电梯产品单一，人梯交互方式亘古不变，新冠疫情肆虐以来，人们在电梯里按键时易病毒感染。而智能化、免接触的电梯可有效阻止病毒传染。选用以Linux作为核心架构的树莓派，通过人工智能、大数据、物联网等领域的运用，开发了一套“免接触式”电梯控制系统。将传统需要手动点击的物理按键替换为具备语音控制、人脸检索、语音合成、NFC近场通信，创建了多种新型的电梯控制方案。并将必要信息通过OLED进行实时显示，继而可在桌面或移动终端进行监控或指令下发。拥有网页、iOS和安卓端操作软件。测试表明，无接触智慧电梯系统功能完善、运行稳定，为社区、公共场所、医院等的民众构建起生命安全防线。     

Charge Booster Tags for Controlled Release of Therapeutics from a Therapeutic Carrier

Injectable hydrogels are promising delivery vehicles for the sustained release of therapeutic proteins. Electrostatic interactions between proteins and hydrogels often increase affinity to decelerate protein release. However, this approach is not suitable for weakly charged proteins. The current study shows that the genetic fusion of a highly charged protein segment (charge booster tag) with proteins can control their interactions with injectable gels. A positive or negative charge booster tag is introduced into urate oxidase (UOX), a therapeutic protein for gout, to generate UOX variants with varying net charges. When a positively-charged injectable hydrogel is used, both the in vitro release rate and in vivo serum half-life of UOX are correlated with the net negative charge. This modified delivery approach results in a serum half-life of over 106 h for the UOX variant, which is substantially longer than that of free UOX (3.3 h). Hence, charge booster tags can be used as a systematic strategy for controlling the release of therapeutic proteins.     

HaloTag-Based Reporters for Fluorescence Imaging and Biosensing

Visualizing the structure and dynamics of biomolecules is critical to understand biological function, and requires methods to fluorescently label targets of interest in their cellular context. Self-labelling proteins, which combine a genetically encoded tag with a small-molecule fluorophore, have attracted considerable attention for this purpose, as they can overcome limitations of fluorescent proteins. Among them, the HaloTag protein is the most broadly used, showing fast specific labelling with a small, easy to functionalize and cell-permeant ligand. Synthetic chemistry and protein engineering have provided a portfolio of powerful imaging tools exploiting HaloTag, along with general methods to optimize and adapt them to specific applications. Here, we provide an overview of fluorescent reporters based on the HaloTag protein for imaging and biosensing, highlighting engineering strategies and general applications.