热水器温度控制板设计

以89C52单片机为核心,设计了热水器温度控制板,从硬件结构和软件设计方面阐述了该系统的实现原理,给出了系统相关的电路原理图和程序框图。该热水器温度控制板实现了温度测量、温度设定、实时显示水温、缺水报警、定时关机等功能。并设计了故障报警功能,大大方便了用户的使用,提高了热水器的安全性。     

模拟裂变中子源能谱测量

一、引言在研究链式反应系统的各种性质时,常常用到具有类似裂变中子能谱的中子源。模拟裂变中子源主要由~(210)Po的α粒子轰击F,Be,Li,B等轻元素产生中子,随着靶元素成份以及结构的差异,中子能谱也不完全相同。应本院同位素处的要求,我们测量了一模拟裂变中子源的能谱,其源强约10~4n/s,靶成份为天然Li 24.16%,F66.14%,B 9.4%,     

次模拟裂变中子源能谱测量

一、引言次模拟和模拟裂变中子源用于研究链式反应系统的各种性质,所以这些中子源的能谱测量是很重要的。源材料、源包壳材料和工艺过程不同,中子源能谱也不同,因而在制作过程中,对源中子能谱的监测是十分必要的。这些中子源的能量一般是几百keV,这一能区连续中子能谱测量一直是很困难的。中子飞行时间法不适于本工作;阈探测器活化法在     

Modification of PROMETHEUS Reactor as a Fusion Breeder and Fission Product Transmuter

This study presents the analyses of the fissile breeding and long-lived fission product (LLFP) transmutation potentials of PROMETHEUS reactor. For this purpose, a fissile breeding zone (FBZ) fueled with the ceramic uranium mono-carbide (UC) and a LLFP transmutation zone (TZ) containing the ⁹⁹TC and ¹²⁹I and ¹³⁵Cs isotopes are separately placed into the breeder zone of PROMETHEUS-H design. The neutronic calculations are performed by using two different computer codes, the XSDRNPM/SCALE4.4a neutron transport code and the MCNP4B Monte Carlo code. A range of analyses are examined to determine the effects of the FF, the fraction of ⁶Li in lithium (Li) and the theoretical density (TD) of Li₂O in the tritium breeder zone (TBZ) on the neutronic parameters. It is observed that the numerical results obtained from both codes are consistent with each other. It is carried out that the profiles of fission power density (FPD) are flattened individually for each FF (from 3 to 10%). Only, in the cases of FF ≥ 8%, the system is self sufficient from the point of view of tritium generation. The results bring out that the modified PROMETHEUS fusion reactor has capabilities of effective fissile breeding and LLFP transmutation, as well as the energy generation.     

Vectors and gene targeting modules for tandem affinity purification in Schizosaccharomyces pombe

We describe the construction of tagging cassettes and plasmids for tandem affinity purification (TAP) of proteins in Schizosaccharomyces pombe. The tagging cassettes are designed for either carboxy- or amino-terminal tagging of proteins. The carboxyl terminal tags differ in that they contain either two or four repeats of IgG binding units. For tagging endogenous loci, the cassettes contain the kan MX6 module to allow for selection of G418-resistant cells. The amino-terminal tagging vectors allow for the regulated expression of proteins. Sz. pombe Cdc2p was chosen to test these new affinity tags. Several known binding proteins co-purified with both Cdc2p-CTAP and N-TAP-Cdc2p, indicating the usefulness of these tags for the rapid purification of stable protein complexes from Sz. pombe.     

Rec10- and Rec12-independent recombination in meiosis of Schizosaccharomyces pombe

The Rec10 protein, a component of the linear elements forming along sister chromatids in meiotic prophase of Schizosaccharomyces pombe, plays an important role in the activation of Rec12 for double-strand break formation, and thus the initiation of recombination between homologous chromosomes. Recombination between homologous chromosomes was moderately reduced in homozygous crosses of the C-terminal truncation mutant rec10-155 and strongly in the full deletion allele rec10-175. Both alleles were also tested in two assays for intrachromosomal recombination (PS1 and VL1) and showed only slight reductions, while deletion of rec12 led to a 13-fold reduction. The even stronger reductions in rec10 rec12 double deletion crosses indicate partially redundant functions of Rec10 and Rec12 in the initiation of intrachromosomal recombination. A low level of double-strand breaks has been detected in rec10-175 meiosis at the mbs1 hotspot of recombination, and spore viability in the double mutant was also lower than in the single-deletion mutants. Low levels of apparent crossover and conversion between homologous chromosomes in the absence of Rec12 have been quantified using a newly developed assay. The results also indicate that the functions of Rec10 differ in several respects from those of its distant homologue Red1 in Saccharomyces cerevisiae, including interactions with Hop1 and Mek1 for promotion of recombination between homologues at the expense of sister chromatid recombination.     

Helicase activity is only partially required for Schizosaccharomyces pombe Rqh1p function

The RecQ-related family of DNA helicases is required for the maintenance of genomic stability in organisms ranging from bacteria to humans. In humans, mutation of three RecQ-related helicases, BLM, WRN and RecQL4, cause the cancer-prone and premature ageing diseases of Bloom syndrome, Werner's syndrome and Rothmund-Thompson syndrome, respectively. In the fission yeast Schizosaccharomyces pombe, disruption of the rqh1(+) gene, which encodes the single Sz. pombe RecQ-related helicase, causes cells to display reduced viability and elevated levels of chromosome loss. After S-phase arrest or DNA damage, cells lacking rqh1(+) function display elevated levels of homologous recombination and defective chromosome segregation. Here we show that, like other RecQ family members, the Rqh1p protein displays 3' to 5' DNA helicase activity. Interestingly, however, unlike other RecQ family members, the helicase activity of Rqh1p is only partially required for its function in recovery from S-phase arrest or DNA damage. We also report that high cellular levels of Rqh1p result in lethal chromosome segregation defects, while more moderate levels of Rqh1p cause significantly elevated rates of chromosome loss. This suggests that careful regulation of RecQ-like protein levels in eukaryotic cells is vital for maintaining genome stability.     

Role of Tea1p, Tea3p and Pom1p in the determination of cell ends in Schizosaccharomyces pombe

Schizosaccharomyces pombe cells are rod-shaped and grow along a single axis from their two ends. Microtubules extend from the cell centre terminating at the cell ends. The ERM(ezrin/radixin/moesin)-like proteins Tea1p and Tea3p, and the Dyrk-like kinase Pom1p are cell end markers involved in the regulation of growth and microtubular dynamics at the cell ends. We have analysed the relative contribution of these three proteins to the determination of cell ends as sites both for cell growth and for microtubular termination. Pom1Delta, in combination with Tea1Delta or Tea3Delta, has the greatest difficulty in relocalizing actin to the cell ends following actin depolymerization and generates the most defective growth pattern. Tea1Delta, in combination with Pom1Delta or Tea3Delta, displays the highest number of microtubules bending round the cell ends. Tea1DeltaPom1Delta, which has the most defective growth pattern and microtubules, also displays the highest number of branched cells. We show that Tea1p, Tea3p and Pom1p all contribute, to different extents, to the determination of cell ends, as sites for both cell growth and microtubular termination. We also show that the fission yeast cell relies on both the positioning of landmarks and a properly organized microtubule cytoskeleton to direct cell growth.