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排序方式: 共有793条查询结果,搜索用时 15 毫秒
41.
Keita Aoki Reiko Nakajima Kanji Furuya Hironori Niki 《Yeast (Chichester, England)》2010,27(12):1049-1060
Schizosaccharomyces japonicus is a fission yeast for which new genetic tools have recently been developed. Here, we report novel plasmid vectors with high transformation efficiency and an electroporation method for Sz. japonicus. We isolated 44 replicating segments from 12 166 transformants of Sz. japonicus genomic fragments and found a chromosomal fragment, RS1, as a new replicating sequence that conferred high transformation activity to Sz. japonicus cells. This sequence was cloned into a pUC19 vector with ura4+ of Sz. pombe (pSJU11) or the kan gene on the kanMX6 module (pSJK11) as selection markers. These plasmids transformed Sz. japonicus cells in the early‐log phase by electroporation at a frequency of 123 cfu/µg for pSJK11 and 301 cfu/µg for pSJU11, which were higher than previously reported autonomously replicating sequences. Although a portion of plasmids remained in host cells by integration into the chromosome via RS1 segment, the plasmids could be recovered from transformants. The plasmid copy number was estimated to be 1.88 copies per cell by Southern blot analysis using a Sz. pombe ura4+ probe. The plasmid containing ade6+ suppressed the auxotrophic growth of the ade6‐domE mutant, indicating that the plasmid would be useful for suppressor screening and complementation assays in Sz. japonicus. Furthermore, pSJU11 transformed Sz. pombe cells with the same frequency as the pREP2 plasmid. This study is a report to demonstrate practical use of episomal plasmid vectors for genetic research in Sz. japonicus. RS1 has been submitted to the DDBJ/EMBL/GenBank database (Accession No. AB547343). Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
42.
用随机中子场下的几率细致平衡主方程讨论了探测器效应对测量结果产生的影响,提出了可用增加探测器对中子的作用几率来减少影响,提高精度。 相似文献
43.
以89C52单片机为核心,设计了热水器温度控制板,从硬件结构和软件设计方面阐述了该系统的实现原理,给出了系统相关的电路原理图和程序框图。该热水器温度控制板实现了温度测量、温度设定、实时显示水温、缺水报警、定时关机等功能。并设计了故障报警功能,大大方便了用户的使用,提高了热水器的安全性。 相似文献
44.
一、引言在研究链式反应系统的各种性质时,常常用到具有类似裂变中子能谱的中子源。模拟裂变中子源主要由~(210)Po的α粒子轰击F,Be,Li,B等轻元素产生中子,随着靶元素成份以及结构的差异,中子能谱也不完全相同。应本院同位素处的要求,我们测量了一模拟裂变中子源的能谱,其源强约10~4n/s,靶成份为天然Li 24.16%,F66.14%,B 9.4%, 相似文献
45.
一、引言次模拟和模拟裂变中子源用于研究链式反应系统的各种性质,所以这些中子源的能谱测量是很重要的。源材料、源包壳材料和工艺过程不同,中子源能谱也不同,因而在制作过程中,对源中子能谱的监测是十分必要的。这些中子源的能量一般是几百keV,这一能区连续中子能谱测量一直是很困难的。中子飞行时间法不适于本工作;阈探测器活化法在 相似文献
46.
This study presents the analyses of the fissile breeding and long-lived fission product (LLFP) transmutation potentials of
PROMETHEUS reactor. For this purpose, a fissile breeding zone (FBZ) fueled with the ceramic uranium mono-carbide (UC) and
a LLFP transmutation zone (TZ) containing the 99TC and 129I and 135Cs isotopes are separately placed into the breeder zone of PROMETHEUS-H design. The neutronic calculations are performed by
using two different computer codes, the XSDRNPM/SCALE4.4a neutron transport code and the MCNP4B Monte Carlo code. A range
of analyses are examined to determine the effects of the FF, the fraction of 6Li in lithium (Li) and the theoretical density (TD) of Li2O in the tritium breeder zone (TBZ) on the neutronic parameters. It is observed that the numerical results obtained from both
codes are consistent with each other. It is carried out that the profiles of fission power density (FPD) are flattened individually
for each FF (from 3 to 10%). Only, in the cases of FF ≥ 8%, the system is self sufficient from the point of view of tritium
generation. The results bring out that the modified PROMETHEUS fusion reactor has capabilities of effective fissile breeding
and LLFP transmutation, as well as the energy generation. 相似文献
47.
48.
Vectors and gene targeting modules for tandem affinity purification in Schizosaccharomyces pombe 总被引:9,自引:0,他引:9
We describe the construction of tagging cassettes and plasmids for tandem affinity purification (TAP) of proteins in Schizosaccharomyces pombe. The tagging cassettes are designed for either carboxy- or amino-terminal tagging of proteins. The carboxyl terminal tags differ in that they contain either two or four repeats of IgG binding units. For tagging endogenous loci, the cassettes contain the kan MX6 module to allow for selection of G418-resistant cells. The amino-terminal tagging vectors allow for the regulated expression of proteins. Sz. pombe Cdc2p was chosen to test these new affinity tags. Several known binding proteins co-purified with both Cdc2p-CTAP and N-TAP-Cdc2p, indicating the usefulness of these tags for the rapid purification of stable protein complexes from Sz. pombe. 相似文献
49.
The Rec10 protein, a component of the linear elements forming along sister chromatids in meiotic prophase of Schizosaccharomyces pombe, plays an important role in the activation of Rec12 for double-strand break formation, and thus the initiation of recombination between homologous chromosomes. Recombination between homologous chromosomes was moderately reduced in homozygous crosses of the C-terminal truncation mutant rec10-155 and strongly in the full deletion allele rec10-175. Both alleles were also tested in two assays for intrachromosomal recombination (PS1 and VL1) and showed only slight reductions, while deletion of rec12 led to a 13-fold reduction. The even stronger reductions in rec10 rec12 double deletion crosses indicate partially redundant functions of Rec10 and Rec12 in the initiation of intrachromosomal recombination. A low level of double-strand breaks has been detected in rec10-175 meiosis at the mbs1 hotspot of recombination, and spore viability in the double mutant was also lower than in the single-deletion mutants. Low levels of apparent crossover and conversion between homologous chromosomes in the absence of Rec12 have been quantified using a newly developed assay. The results also indicate that the functions of Rec10 differ in several respects from those of its distant homologue Red1 in Saccharomyces cerevisiae, including interactions with Hop1 and Mek1 for promotion of recombination between homologues at the expense of sister chromatid recombination. 相似文献
50.