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11.
一个巨大的抗真菌抗生素基因簇及其与植物生物技术的潜在关系 总被引:1,自引:0,他引:1
从链霉菌FR-008中克隆出一个七烯大环内酯抗生素的生物合成基因簇,基因置换试验证实克隆的基因片段与抗生素生物合成直接相关。FR-008抗生素含有一个38员由聚酮所衍生的内酯大环,由编码红霉素聚酮合酶的活性域基因片段为探针所进行的Southern杂交试验证明,链霉菌FR-008的聚酮合酶(PKS)的基因簇占据105kb连续和具有重复功能单元(模块)的序列;假设每个PKS模块为5kb,这与FR-008碳链形成需要21个缩合过程的预期数值极其吻合。链霉菌FR-008的基因克隆系统(包括转化、转座、从大肠杆菌向链霉菌的属间接合转移、基因置换和基因中断体系和限制性缺陷菌株)均已得到发展,使我们能够充分利用基因工程技术来操纵七烯大环内酯的生物合成并产生新的抗生素衍生物。同时利用该基因簇中的部分PKS基因序列构建了一个可用于转化植物的质粒pHZ321,为尝试在水稻等植物中表达链霉菌Ⅰ型PKS基因以探索高G十C(76%)含量的链霉菌PKS基因能否在植物中表达提供了材料和工具。用此质粒来转化植物的研究,将为进一步利用这个巨大的抗真菌抗生素基因簇进行植物抗真菌的基因工程育种提供理论依据。 相似文献
12.
本文研究了聚氯乙烯在碱金属叔丁醇溶液中脱氯化氢,研究了时间、反应试剂及溶剂对反应的影响。用FTIR和UV—Vis光谱表征产物的结构并测定了产物掺杂前后的电导率,得到具有较高转化率及共轭链段类反式聚乙炔结构的产物。 相似文献
13.
Xiaoming Liu Xiang RuYao Li Kaka ZhangDaoyong Chen 《International Journal of Hydrogen Energy》2011,36(16):9612-9619
A diiron hexacarbonyl complex possessing an alkynyl group as a model complex of the diiron sub-unit of [FeFe]-hydrogenase was polymerized under the catalysis of WCl6-SnPh4. The polymer (Poly-{Fe2}) functionalized with {Fe2(CO)6} units which is dominated by cis-form was fully characterised using FTIR, NMR, SEM, TEM, and TGA techniques. Through modifying the monomer, the properties, for example, solubility, of the resultant polymer could be tuned and much larger molecule weight, which was estimated as 7.93 × 105 g mol−1 using static light scattering technique was achieved without compromising its solubility. Spin-coating the functionalized polymer onto the surface of vitreous carbon electrode with or without multi-wall carbon nanotubes (MWCNTs) produced film electrodes which show electrochemical responses. Adding MWCNTs into the film enhances significantly the electrochemical response probably via not only improving the conductivity of the film, but also the increase in its effective surface area after being doped with MWCNTs. 相似文献