Use of a YAP1 overexpression cassette conferring specific resistance to cerulenin and cycloheximide as an efficient selectable marker in the yeast Saccharomyces cerevisiae.

Drug-resistance markers for yeast transformation are useful because they can be applied to strains without auxotrophic mutations. However, they are susceptible to technical difficulties, namely lower transformation efficiency and the appearance of drug-resistant mutants without the marker. To avoid these problems, we have constructed a phosphoglycerate kinase (PGK) promoter-driven YAP1 expression cassette, called PGKp-YAP1. Yeast cells containing PGKp-YAP1 were resistant to cycloheximide, a protein synthesis inhibitor, and also to cerulenin, a fatty acid synthesis inhibitor, but not to other drugs tested. The transformation efficiency of PGKp-YAP1 using cerulenin selection was comparable to that using a URA3 auxotrophic marker when low concentrations of cerulenin were used. Non-transformed drug-resistant colonies did appear on the low-concentration cerulenin plates. However, these non-transformed colonies could easily be identified, based on their cycloheximide sensitivity and/or their resistance to aureobasidin A to which the transformants were sensitive. Therefore, the dual drug resistance of PGKp-YAP1 could be used as an effective selection for PGKp-YAP1 recipient cells. The PGKp-YAP1 marker was used to disrupt the LYS2 gene and to transform an industrial yeast strain, indicating that this marker can be used for efficient and reliable gene manipulations in any Saccharomyces cerevisiae strain.     

10/100Mbps以太网物理层收发器DM9101的原理与应用

MD9101是Davicom公司开发研制的基于10／100Mbps以太网物理层的单片低功耗收发器，文中介绍了DM9101的特点，引脚功能和工作原理，并给出一种典型的应用设计电路。     

高效铣环在胜科1井事故处理中的应用

胜科1井是中国石化的一口重点科学探井,在钻至井深5001m时因井眼缩径发生了卡钻事故。由于井底存在直翼扩大器、PDC钻头等含有硬质合金的落鱼,普通铣鞋无法完成套铣打捞作业,为此,专门设计了一种高效铣环。该铣环的切削元件选用了一种硬度达93.2 HRA的钛合金,经铣槽镶焊在本体上;本体选用了40CrNiMo厚壁管材,并进行了调质处理;连接螺纹选用了双级梯形同步螺纹。在胜科1井的应用表明：切削元件的强度满足了切削扩大器切削翼和PDC钻头胎体的需要,其齿形、布齿方向和出刃高度设计合理,提高了切削速度;本体性能高,切削元件焊接性能较好;双级梯形同步螺纹有效增加了连接部位的强度,具有较高的上扣扭矩,承受冲击载荷能力强。高效铣环在该井的成功应用,为今后同类型井的事故处理提供了借鉴。     

硫脲树脂对Cu~(2+)吸附性能研究

考察了不同吸附时间、pH值和吸附温度下,聚对苯甲酰基硫脲树脂和聚酯基硫脲树脂对Cu2+的吸附性能影响;并对吸附动力学和热力学进行了研究。     

家庭智能终端系统的硬件设计与实现

随着生活水平的提高,人们对居住环境的舒适性、安全性要求不断提高。家庭自动化、智能化已经成为自动化领域的一个发展热点。本文将当前比较先进的ARM技术运用到家庭智能终端系统的开发上,设计实现了一个既适合当前家庭需要又符合今后发展方向的家庭智能终端体系结构,较好地解决了绝大多数智能家居系统所普遍存在的实时性差、安全性低等问题。     

胜科1井钻井事故的预防与处理

胜科1井设计井深7 000 m,完钻井深7 026 m。在该井钻井过程中,针对上部大尺寸钻头破岩效率低、大井眼井壁稳定性差、大段盐膏层、膏岩层的塑性蠕变和塑性流动造成井眼复杂、超深井高温高压给钻井液性能的控制和水泥浆调控等带来的一系列技术难题,制定了一整套预防井下事故和复杂情况的技术措施,从而确保了该井的钻探成功。该井在钻井过程发生了2次断钻具事故和1次卡钻事故,根据超深井的特点,采取了较为稳妥的处理措施,同时,有针对性地研制了高效铣环和PDC钻头专用打捞器,3次事故处理均获得了成功,共耗时38.5 d。详细介绍了该井所采取的预防和处理钻井事故的技术措施,可为其他超深井的钻井施工提供一定的借鉴。     

Oxygen glow discharge experiment to remove deposited layers and to release trapped hydrogen isotopes in HT-7 superconducting tokamak

An experiment to remove re-deposited layers and to release hydrogen using a glow discharge in oxygen (O-GDC) has been performed in the HT-7 superconducting tokamak. In the absence of magnetic fields, the O-GDC wall conditioning had produced rapid, controlled co-deposit removal. Average removal rates, 5.2 × 10²² H-atoms/h, 5.65 × 10²¹ D-atoms/h and 5.53 × 10²² C-atoms/h, respectively, were obtained during 145 min O-GDC experiment in the pressure range 0.5-1.5 Pa. The corresponding removal rate of co-deposited films was ∼1.19 μm/day (26.5 g/day for carbon) based on an area of 12 m². Compared to thermo-oxidation and O-ICR experiment, high pressure O-GDC wall conditioning promoted the oxidation and improved the C and D atoms removal. In the O-GDC experiment, the removal rates of H-atoms and D-atoms as H₂O, HDO and D₂O were higher than that of H₂ and D₂ by factors of about 20 and 50, respectively. During the 145 min O-GDC experiment, about 14.5% O-atoms were converted into carbon oxides and hydroxides, and about 5.37 × 10²² O-atoms were adsorbed on the walls corresponding to a coverage of 4.5 × 10²¹ O/m² on an wall area of 12 m². In a 100 min helium glow discharge (He-GDC) following the O-GDC experiment, 1.53 × 10²² O-atoms, about 28.5% oxygen retained on the walls, were removed. The removal rate of H-atoms in He-GDC cleaning after O-GDC experiment was lower than that in He-GDC cleaning before O-GDC experiment, which indicates that the O-GDC wall conditioning had effectively reduced hydrogen retention on the walls.     

Formation of steady state size distribution of precipitates in alloys under cascade-producing irradiation

The effect of coherency loss on the development of precipitate size distribution under cascade-producing irradiation is considered. The nucleation of coherent precipitates, their growth followed by coherency loss and cascade-induced dissolution of large incoherent precipitates can occur simultaneously resulting in formation of a quasi-stationary size distribution of semicoherent precipitates. To describe this process we consider co-evolution of a mixed population of coherent, semicoherent and incoherent precipitates. Mathematically, the problem is formulated as a set of discrete rate equations of nucleation kinetics (the Master equation approach) which is also used for later stages of evolution. To solve the corresponding large set of equations (typically, more than 10⁵ equations) an efficient numerical method is developed. The simulation results obtained for material parameters and irradiation conditions typical for nuclear reactors show that the coherency loss affects considerably evolution of the precipitate population. Under certain irradiation conditions, both in solution-annealed alloys and in aged ones, the mean precipitate size and the number density during prolonged irradiation tend to steady state values, whereas the size distribution function of large precipitates narrows. The width of the quasi-stationary size distribution is controlled by cascade parameters. It was found that the asymptotic quasi-stationary state of the precipitate population may depend on initial state of the alloy.     

活性污泥数学模型入流组分测定及应用

阐述了活性污泥1号模型人流组分的分析方法，并用这些方法测定了西安市城市污水水质。实验结果表明测定方法操作简单、准确可靠，能为利用模型进行污水处理厂的设计、模拟及管理提供入流组分分析依据，