A Novel Cartesian Plot Analysis for Fixed Monolayers That Relates Cell Phenotype to Transfer of Contents between Fibroblasts and Cancer Cells by Cell-Projection Pumping

We recently described cell-projection pumping as a mechanism transferring cytoplasm between cells. The uptake of fibroblast cytoplasm by co-cultured SAOS-2 osteosarcoma cells changes SAOS-2 morphology and increases cell migration and proliferation, as seen by single-cell tracking and in FACS separated SAOS-2 from co-cultures. Morphological changes in SAOS-2 seen by single cell tracking are consistent with previous observations in fixed monolayers of SAOS-2 co-cultures. Notably, earlier studies with fixed co-cultures were limited by the absence of a quantitative method for identifying sub-populations of co-cultured cells, or for quantitating transfer relative to control populations of SAOS-2 or fibroblasts cultured alone. We now overcome that limitation by a novel Cartesian plot analysis that identifies individual co-cultured cells as belonging to one of five distinct cell populations, and also gives numerical measure of similarity to control cell populations. We verified the utility of the method by first confirming the previously established relationship between SAOS-2 morphology and uptake of fibroblast contents, and also demonstrated similar effects in other cancer cell lines including from melanomas, and cancers of the ovary and colon. The method was extended to examine global DNA methylation, and while there was no clear effect on SAOS-2 DNA methylation, co-cultured fibroblasts had greatly reduced DNA methylation, similar to cancer associated fibroblasts.     

量表精度自动检定仪的研制

对应用图像识别技术进行量表精度检定的测量原理进行了理论分析,研究了成像系统的组成及参数选择、被测物图像的采集、图像的处理及相关算法等,并据此研制了量表精度自动检定系统。实际应用结果表明,该检定仪性能稳定,测量精度较高,可满足工业量表检定的要求。     

Intelligent information acquisition methods and seismic damage prediction of rural masonry building groups

我国村镇地区砌体结构分布广、数量大、抗震性能普遍较差，且具有结构形式多样、无数据和随机性强等特点，获取其建筑信息是实现震害预测的前提和基础，但传统人工检测技术效率低、不经济。为此，提出了一种图像识别与模糊推理相结合的村镇砌体建筑群信息智能获取方法。建立了基于卷积神经网络的砌体建筑图像识别模型，结合图像测量技术获取建筑几何信息；提出了基于模糊推理的砌体结构隐蔽信息确定方法，可有效提取村镇砌体结构墙体属性、构造措施、材料强度等隐蔽属性信息；依据获取的建筑信息与场地信息，分别建立单体结构力学模型与空间地震动场模型，通过结构地震动力响应分析实现村镇区域砌体建筑群的震害预测。最后，对北方地区某村落进行群体建筑信息获取和震害预测，结果表明,基于影像数据的图像识别和图像测量技术可以有效获取村镇建筑结构类型以及外观尺寸信息,提出的模糊推理方法可以高效、准确地识别砌体建筑群的隐蔽信息，进一步通过对震害场景的数值模拟，实现村镇区域群体建筑震害的有效预测。     

HREM image simulations for small particle catalysts on crystalline supports

The imaging conditions for electron microscope studies of supported ultrafine particle catalysts have been investigated by multislice simulations. Images of Pt and ReO₄ particles ranging from 0·4 to 2·3 nm in size were simulated in both plan view and profile view with a rutile (TiO₂) support. It was shown that particle visibility varied greatly with the objective lens defocus. Optimum defocus was not favourable for supported particles in plan view since the ultrafine supported particles were least visible at this defocus. Underfocusing, especially at defoci corresponding to half-spacing fringes in the TiO₂ support, led to improved visibility and resolution of the supported particles. Although the structure and shape of supported ultrafine particles should be resolved better with a 400-kV high-resolution electron microscope, their detectability is poorer than with a 200-kV instrument. An ReO₄ cluster should be detectable at 200 kV on TiO₂ supports up to 5 nm in thickness, whereas it is only likely to be detectable at 400 kV on supports up to 3 nm in thickness. The simulations confirmed that optimum defocus is most favourable for imaging supported particles in profile view. Atomic information for particles as small as a 13-atom Pt cuboctahedral cluster should be resolvable with a 400-kV instrument. The crystalline Ti monolayer observed on surfaces of Pt particles, which could explain the mechanism known as SMSI, was simulated as an example of profile imaging.     

基于相关系数的光流计算方法研究

在对移动机器人的运动与光流进行定性分析时,引入全局光流相关系数的概念,将相关系数作为光流全局分布特牲的测度因子来判别移动机器人运动情况。这对以后的移动机器人视觉导航等具有一定的现实意义。     

直线超声电动机驱动的平面3-PRR并联平台视觉精密定位

提出一种直线超声电动机驱动的平面3-PRR并联平台,研究并联平台视觉精密定位技术。给出并联平台结构并采用矢量链法得到并联平台运动学模型。针对并联平台动平台位姿测量问题,利用视觉系统作为反馈单元,通过图像处理技术对动平台上的人工特征中心定位,实现对动平台位姿精确测量;针对传统PID关节控制器在实际控制中问题,采用基于扰动观测器的并联平台支链控制器模型。试验结果表明：对于目标位置为(20 mm,20 mm),并联平台半闭环控制精度约为±500 µm,而通过视觉定位精度达到±5 µm。视觉定位较半闭环控制大幅提高了并联平台定位精度,能够满足并联平台大行程、高精度定位的需求。     

Adapting an ultrasonic image threshold method to eddy current images and defining a validation domain of the thresholding method

This paper presents a generalization to eddy current images of a thresholding method developed for ultrasonic imaging. It is also shown how to improve the thresholding strength through the definition of a cooperation rule between two threshold assessment measures. This rule is also used to reduce time of calculus. These measures are calculated from the image cooccurrence matrix. The aim is to build a thresholding method that gives a threshold value but also an indicator which allows the operator to know if the methodology applies to the processed image. Examples of results are presented.     

数字图像相关法的板料成形极限研究与应用

为了克服现有板料成形极限测定方法的不足,采用XJTUDIC三维数字散斑应变测量系统,实现了一种基于数字图像相关法的板料成形极限测定新方法.首先,将测量系统架构在杯突试验机上,记录从试验过程初始到破裂的一系列图像,实现应变在线、全场测定.然后,通过对破裂前的最后一张图像进行截线,拟合数据,对颈缩区域插值得到极限应变,从而绘制成形极限曲线图.最后,通过对SPCC36型号碳钢材料板料件成形极限的测量,验证了本文方法的可行性和有效性.     

Progression and Dissemination of Pulmonary Mycobacterium Avium Infection in a Susceptible Immunocompetent Mouse Model

Pulmonary infections caused by the group of nontuberculosis mycobacteria (NTM), Mycobacterium avium complex (MAC), are a growing public health concern with incidence and mortality steadily increasing globally. Granulomatous inflammation is the hallmark of MAC lung infection, yet reliable correlates of disease progression, susceptibility, and resolution are poorly defined. Unlike widely used inbred mouse strains, mice that carry the mutant allele at the genetic locus sst1 develop human-like pulmonary tuberculosis featuring well-organized caseating granulomas. We characterized pulmonary temporospatial outcomes of intranasal and left intrabronchial M. avium spp. hominissuis (M.av) induced pneumonia in B6.Sst1S mice, which carries the sst1 mutant allele. We utilized traditional semi-quantitative histomorphological evaluation, in combination with fluorescent multiplex immunohistochemistry (fmIHC), whole slide imaging, and quantitative digital image analysis. Followingintrabronchiolar infection with the laboratory M.av strain 101, the B6.Sst1S pulmonary lesions progressed 12–16 weeks post infection (wpi), with plateauing and/or resolving disease by 21 wpi. Caseating granulomas were not observed during the study. Disease progression from 12–16 wpi was associated with increased acid-fast bacilli, area of secondary granulomatous pneumonia lesions, and Arg1+ and double positive iNOS+/Arg1+ macrophages. Compared to B6 WT, at 16 wpi, B6.Sst1S lungs exhibited an increased area of acid-fast bacilli, larger secondary lesions with greater Arg1+ and double positive iNOS+/Arg1+ macrophages, and reduced T cell density. This morphomolecular analysis of histologic correlates of disease progression in B6.Sst1S could serve as a platform for assessment of medical countermeasures against NTM infection.     

Study on the forecast method for underground coal mine

At present, coal mine fires were forecasted with some temperature, smog, CO, CO2, etc, however, this method can＇t meet the requirements for safe production of coal mines in monitoring accuracy and validity. Overcoming these problems of foregone monitoring methods, using multi-parameters which include fire image, smog, CO, CO2, O2, etc, the paper put forward a synthetical analysis monitor with advanced technology of neural network. The research and application of this method has significance in theory and practical value for coal mine fire forecast.