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131.
A protein which masks galactose receptor mediated phage susceptibility in Lactococcus lactis subsp. lactis MPL56 总被引:1,自引:0,他引:1
Yasin Tuncer & Mustafa Akçelik 《International Journal of Food Science & Technology》2002,37(2):139-144
A 28.5-kb plasmid, isolated from Lactococcus lactis subsp. lactis MPL56, causes complete inhibition of four lactococcal phages. Cell wall characteristics of wild-type strain MPL56 were compared with its 28.5 kb plasmid-cured, phage-sensitive derivative MPL56-22. After proteolytic enzyme treatments, adsorption of phages occurred at high levels, an example is 94.6–98.5% in MPL56 cells. Analysis of cell wall extracts of MPL56-22 by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) indicated that the only difference between strains was the 55.4 kDa band in protein patterns of MPL56. Adsorption of the four phages was completely inhibited when MPL56-22 cells were subjected to SDS, Triton-X-100, HCl and NaOH treatments. Lectins that were specific for glucose/mannose and N-acetylglucosamine did not prevent adsorption of phages in cell wall extracts of MPL 56-22. However a lectin specific for galactose (MCA; Momordica charantia ) completely inhibited adsorption of these phages in cell wall extracts of MPL56-22. HPLC patterns of cell wall carbohydrates of MPL56-22 and its HCl treated preparations showed that the most prevalent difference was the galactose on untreated MPL56-22 cell wall chromatograms. 相似文献
132.
从分析某高架桥中异形板板底裂缝的特点及成因着手 ,对设计与施工工艺诸方面提出防止裂缝的对策和对裂缝进行加固的全过程。 相似文献
133.
球磨机衬板材料及热处理工艺 总被引:1,自引:0,他引:1
对传统的中碳低合金耐磨铸钢的化学成分和热处理工艺进行了优化设计 ,使新材料具有较高的强度、硬度和韧性 ,因而抗冲击、抗疲劳、抗磨损性能良好。实际应用表明 ,用此种材料代替油淬和风冷中碳低合金耐磨铸钢 ,不仅生产成本低 ,而且在矿山、电力球磨机制粉等工矿条件下 ,其使用寿命长 相似文献
134.
Birthe Mller Jose Luis Molina‐Cano Lars Munck 《Journal of the Institute of Brewing》2002,108(3):294-302
In earlier studies concerning vigour, where subsamples are heat‐treated before germination there was found heat‐sensitive as well as heat‐resistant barley samples. The vigour model developed by Ellis and Roberts and further developed at Carlsberg, could only describe the heat‐sensitive barleys. Seventeen samples of the “Alexis” variety grown widely in Europe were collected from the EBC trials in 1994 in order to see if heat resistance in barley was influenced by different growing conditions. We found both heat‐sensitive samples following the vigour model as well as pronounced heat‐resistant samples, but these were not divided according to growing conditions. The germination curves dependent on heat treatment and germination time were evaluated by Principal Component Analysis (PCA). Heat‐resistant barley samples could be differentiated from heat‐sensitive samples already after ½ h of heat treatment at 68°C (12% moisture) and after 3 days of germination. The barley samples were analysed with regard to malting quality. The PCA evaluation of the data divided the samples according to growing location, mainly due to differences in protein and β‐glucan. However, the malting analyses could not describe the differences in heat resistance and sensitivity of the barleys. The biochemical background of the heat resistance found is discussed on the basis of literature. Our findings should give an experimental basis for exploiting a biochemical principle for heat resistance, which is formed during grain filling and consumed during storage and germination. 相似文献
135.
Witold Brostow Bernard Bujard Patrick E. Cassidy Haley E. Hagg Pablo E. Montemartini 《Materials Research Innovations》2002,6(1):7-12
Samples were prepared by addition of a fluorinated poly(aryl ether ketone) (12F-PEK) to a commercial epoxy resin and curing
at either 24 °C or 70 °C. The concentrations of fluoropolymer in the samples were 0, 5, 10, 20, 30, 40, and 50%. Phase inversion
facilitates the formation of more and more smooth surfaces for scratch testing with increasing fluoropolymer concentration.
A progressively increasing load from 0.03 N to 12 N was administered with a diamond tip to the surfaces of the samples; depths
were determined within ±7.5 nm. The original scratch depth is a function of the fluoropolymer concentration. Scratch recovery
(healing) reaches values up to 95%. Plots of the residual depth versus concentration of the fluoropolymer at 4, 6, 8, and
10 N reveal minima for all forces and both temperatures. Addition of only 5% or 10% 12F-PEK improves the scratch recovery
significantly. The results are explained by changes of material morphology with the fluoropolymer concentration.
Electronic Publication 相似文献
136.
137.
文章叙述了新型镍基耐蚀哈氏合金G-30在不同介质中的耐蚀性能以及工业应用,综合分析了该合金应用的经济性及前景。 相似文献
138.
网带式连续烧结炉炉温及网带速度微机控制系统 总被引:1,自引:0,他引:1
本文介绍一个基于STD总线的单片机网带式连续烧结炉炉温及网带速度控制系统,详细叙述了系统的硬件结构和控制算法。 相似文献
139.
140.
Janne Halme Minna Toivola Antti Tolvanen Peter Lund 《Solar Energy Materials & Solar Cells》2006,90(7-8):872-886
Electrochemical impedance spectroscopy was used to determine the effective charge transfer resistances of porous dye-sensitized solar cell counter electrodes prepared by low-temperature spray deposition and compression of conductive carbon and platinized Sb-doped SnO2 powders on indium tin oxide-coated plastic substrates. The charge transfer resistances were 0.5–2 and 8–13 Ω cm2, respectively, when using 3-methoxypropionitrile as the electrolyte solvent. The manufacturing method used lends itself to produce mechanically stable and even-quality electrodes in an easy and fast manner. 相似文献