全文获取类型
收费全文 | 1403篇 |
免费 | 145篇 |
国内免费 | 18篇 |
专业分类
电工技术 | 4篇 |
综合类 | 30篇 |
化学工业 | 406篇 |
金属工艺 | 7篇 |
机械仪表 | 20篇 |
建筑科学 | 22篇 |
能源动力 | 9篇 |
轻工业 | 903篇 |
水利工程 | 1篇 |
石油天然气 | 18篇 |
无线电 | 13篇 |
一般工业技术 | 41篇 |
冶金工业 | 47篇 |
原子能技术 | 3篇 |
自动化技术 | 42篇 |
出版年
2024年 | 18篇 |
2023年 | 65篇 |
2022年 | 219篇 |
2021年 | 189篇 |
2020年 | 59篇 |
2019年 | 84篇 |
2018年 | 72篇 |
2017年 | 60篇 |
2016年 | 73篇 |
2015年 | 68篇 |
2014年 | 72篇 |
2013年 | 116篇 |
2012年 | 48篇 |
2011年 | 65篇 |
2010年 | 38篇 |
2009年 | 31篇 |
2008年 | 32篇 |
2007年 | 36篇 |
2006年 | 24篇 |
2005年 | 33篇 |
2004年 | 23篇 |
2003年 | 18篇 |
2002年 | 27篇 |
2001年 | 6篇 |
2000年 | 9篇 |
1999年 | 5篇 |
1998年 | 12篇 |
1997年 | 5篇 |
1996年 | 3篇 |
1995年 | 5篇 |
1994年 | 11篇 |
1993年 | 5篇 |
1992年 | 15篇 |
1991年 | 7篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1987年 | 5篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1984年 | 1篇 |
排序方式: 共有1566条查询结果,搜索用时 15 毫秒
101.
建立了中性氧化铝固相萃取柱净化,超高效液相色谱-串联质谱法(UPLC-MS/MS)测定乳和乳制品中黄曲霉毒素M1的分析方法。样品经乙腈提取,乙酸锌辅助沉淀蛋白,中性氧化铝SPE柱净化,以乙腈和0.1%甲酸水溶液作为流动相进行梯度洗脱,以电喷雾离子源(ESI)在正离子多反应监测(MRM)模式下进行测定,外标法定量。结果表明,黄曲霉毒素M1在0.1~50μg/L浓度范围内呈良好的线性关系,相关系数(r2)大于0.99;空白样品加标回收率在70.8%~79.2%之间,相对标准偏差小于8%。方法检出限为0.02μg/kg,定量限为0.05μg/kg。该方法实用、准确、灵敏,适用于乳和乳制品中黄曲霉毒素M1的测定。 相似文献
102.
As explored by biologists, there is a real and emerging need to identify co-regulated gene clusters, which include both positive and negative regulated gene clusters. However, the existing pattern-based and tendency-based clustering approaches are only designed for finding positive regulated gene clusters. In this paper, a new subspace clustering model called g-Cluster is proposed for gene expression data. The proposed model has the following advantages: 1) find both positive and negative co-regulated genes in a shot, 2) get away from the restriction of magnitude transformation relationship among co-regulated genes, and 3) guarantee quality of clusters and significance of regulations using a novel similarity measurement gCode and a user-specified regulation threshold δ, respectively. No previous work measures up to the task which has been set. Moreover, MDL technique is introduced to avoid insignificant g-Clusters generated. A tree structure, namely GS-tree, is also designed, and two algorithms combined with efficient pruning and optimization strategies to identify all qualified g-Clusters. Extensive experiments are conducted on real and synthetic datasets. The experimental results show that 1) the algorithm is able to find an amount of co-regulated gene clusters missed by previous models, which are potentially of high biological significance, and 2) the algorithms are effective and efficient, and outperform the existing approaches. 相似文献
103.
104.
通过转录组学及其生物信息学分析,研究了橄榄苦苷缓解db/db小鼠糖尿病的肝脏差异表达基因及相关信号通路。结果发现与db/db对照组相比,橄榄苦苷处理组的539个基因发生显著变化,其中450个基因显著上调,89个基因显著下调。将上调和下调的差异表达基因在基因本体论数据库中注释,这些差异表达基因主要在细胞过程、细胞部分和结合中分布。京都基因与基因组百科全书通路富集分析结果显示上调的差异表达基因主要富集在磷酸肌醇3-激酶-蛋白激酶B信号通路途径,该通路共涉及27个差异表达基因;下调的差异表达基因主要富集在花生四烯酸代谢和真核生物中核糖体的生物发生信号通路途径,分别涉及4个差异表达基因。本研究为进一步阐明橄榄苦苷缓解2型糖尿病的分子机制奠定了基础。 相似文献
105.
《Food Control》2017
The objective of this study was to investigate the prevalence and characteristics of Listeria monocytogenes isolated from Chinese food, including frozen dumplings, flavored raw meat, roasted meat, braised meat, and a cold vegetable dish with sauce. A total of 900 food samples were collected from supermarkets, open-air markets, and delicatessens in three large cities in the central area of China to examine the presence of L. monocytogenes; 21 (2.3%) of the samples were positive for this pathogen. Among the different samples, braised meat showed the highest L. monocytogenes detection rate (4.4%). Samples obtained from delicatessens showed a much higher L. monocytogenes contamination rate (8.3%) than those from open-air markets (6.7%) or supermarkets (0%). Multilocus sequence typing (MLST) analysis indicated that the 21 bacterial isolates belonged to 12 ST subgroups. ST5 was the largest and contained 7 isolates (33.3%); it was followed by ST474, ST121 and ST9 (each containing 2 isolates [10.5%]). Antibiotic susceptibility analysis showed that the 21 L. monocytogenes isolates were thoroughly resistant to cefoxitin but highly susceptible to doxycycline and ciprofloxacin. The presence of 10 virulence genes was evaluated by PCR, which showed that inlA, inlC, inlJ, prfA, hlyA, and plcB were present in all isolates and that inlB, actA, plcA and iap were present in 71.4–90.5% of the isolates. This study provides a useful reference for risk assessment and control of L. monocytogenes contamination in Chinese food and for the treatment of clinical listeriosis. 相似文献
106.
M.E. Lyn R.M. Zablotowicz B.J. Johnson 《Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment》2013,30(3):381-387
While soil application of a competitive non-toxigenic Aspergillus flavus strains is successful in reducing aflatoxin contamination in certain crops, direct application to aerial reproductive structures could be more effective for maize. A sprayable, clay-based water-dispersible granule formulation was developed to deliver non-toxigenic A. flavus strain K49 directly to maize ears. The efficacy of the K49 water-dispersible granule in mitigating aflatoxin in maize (Zea mays L.) was evaluated. Field studies were conducted to compare K49 colonization and effectiveness in reducing aflatoxin contamination when applied either as a soil inoculant or as a directed spray in plots infested with toxigenic strain F3W4. Fifty percent of non-toxigenic A. flavus was recovered from non-treated controls and from plots soil inoculated with K49 on wheat. In spray treatments with formulated or unformulated K49 conidia, over 90% of A. flavus recovered was non-toxigenic. Soil-applied K49 reduced aflatoxin contamination by 65% and spray applications reduced contamination by 97%. These findings suggest direct spray application of non-toxigenic A. flavus strains may be better than soil inoculation at controlling maize aflatoxin contamination and that a water-dispersible granule is a viable delivery system for maintaining viability and efficacy of the biological control agent, K49. 相似文献
107.
108.
Erik Nordkvist Anna Stepinska Per Häggblom 《Journal of the science of food and agriculture》2009,89(2):359-361
BACKGROUND: Elevated levels of aflatoxin M1 were observed in routine checks of consumer milk in southern Sweden in early 2006. A trace‐back study revealed contaminated milk from several farms, and a total of 68 farms were banned from delivering milk to dairies for shorter or longer periods. The maximum level of aflatoxin M1 in a single sample from an individual farm was 257 ng kg?1 fresh milk. RESULTS: Aflatoxin analyses of commercial compound feed revealed that the contamination originated from the ingredient rice feed meal, a by‐product from the preparation of Basmati rice for human consumption. Up to 56 µg kg?1 of aflatoxin B1 was found in rice feed meal at one feed mill. CONCLUSION: The present example shows that an aflatoxin‐contaminated minor feed ingredient included at less than 10% (w/w) of compound cattle feed can significantly contaminate the milk produced. This emphasises the need for effective monitoring of the feed chain of food‐producing animals in order to prevent food contamination. Copyright © 2008 Society of Chemical Industry 相似文献
109.
110.
Codruţa Ignea Cristina Mihaela Dorobanţu Christopher Paul Mintoff Norica Branza-Nichita Michael R. Ladomery Panagiotis Kefalas Veronica Sanda Chedea 《Food chemistry》2013
Grape seed extracts (GSEs) were investigated in yeast cells harbouring defects in their antioxidant system (regarding the cellular growth and growth recovery from H2O2 insult). GSEs antioxidant activity was detected in wild-type and mutant strains Δcta1, Δgsh1 and Δoye2glr1, while pro-oxidant activity in Δsod1 cells was seen. Assessment of proliferation of prostate cancer PC3 and HBV-replicating HepG2 2.2.15 cells treated with GSEs has shown higher cytotoxicity of red grape seed extract (RW) than white grape seed extract (WW) subjective to dose and period of administration. No antiviral effect was detected by measuring the secreted virion particles in HepG2 2.2.15 cells treated with GSEs. The GSEs play a dual antioxidant/pro-oxidant role in vivo according with the cellular antioxidant system deficiencies and exhibit cytotoxic properties in PC3 and HepG2 2.2.15 cell lines, but no antiviral action against HBV. 相似文献