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91.
92.
小肠RNA对小鼠电离辐射肠道损伤修复的差异基因表达的研究 总被引:3,自引:2,他引:1
为从基因水平探讨小肠糖核酸,促进小鼠电离辐射肠道操作修复的机理,采用随机分组法把90只小鼠分成4组,建立辐射后给予40μgRNA治疗的实验组与0.4mL生理盐水治疗的对照组模型,分别于照射后6、12、24h,4d和8d采集小肠空肠段标本,动用消减杂交基础上的LD-PCR技术,分离实验组与对照组之间的差异表达基因。结果表明:实验组与对照组6、12、24h,4d和8d的克隆新表达基因数分别为18、22 相似文献
93.
Davide Loizzo Savio Domenico Pandolfo Devin Rogers Clara Cerrato Nicola Antonio di Meo Riccardo Autorino Vincenzo Mirone Matteo Ferro Camillo Porta Alessandro Stella Cinzia Bizzoca Leonardo Vincenti Marco Spilotros Monica Rutigliano Michele Battaglia Pasquale Ditonno Giuseppe Lucarelli 《International journal of molecular sciences》2022,23(7)
Autophagy is a complex process involved in several cell activities, including tissue growth, differentiation, metabolic modulation, and cancer development. In prostate cancer, autophagy has a pivotal role in the regulation of apoptosis and disease progression. Several molecular pathways are involved, including PI3K/AKT/mTOR. However, depending on the cellular context, autophagy may play either a detrimental or a protective role in prostate cancer. For this purpose, current evidence has investigated how autophagy interacts within these complex interactions. In this article, we discuss novel findings about autophagic machinery in order to better understand the therapeutic response and the chemotherapy resistance of prostate cancer. Autophagic-modulation drugs have been employed in clinical trials to regulate autophagy, aiming to improve the response to chemotherapy or to anti-cancer treatments. Furthermore, the genetic signature of autophagy has been found to have a potential means to stratify prostate cancer aggressiveness. Unfortunately, stronger evidence is needed to better understand this field, and the application of these findings in clinical practice still remains poorly feasible. 相似文献
94.
Zhiqi Hao Sai Ma Lunping Liang Ting Feng Mengyuan Xiong Shangshu Lian Jingyan Zhu Yanjun Chen Lijun Meng Min Li 《International journal of molecular sciences》2022,23(7)
Drought and salinity stresses are significant abiotic factors that limit rice yield. Exploring the co-response mechanism to drought and salt stress will be conducive to future rice breeding. A total of 1748 drought and salt co-responsive genes were screened, most of which are enriched in plant hormone signal transduction, protein processing in the endoplasmic reticulum, and the MAPK signaling pathways. We performed gene-coding sequence haplotype (gcHap) network analysis on nine important genes out of the total amount, which showed significant differences between the Xian/indica and Geng/japonica population. These genes were combined with related pathways, resulting in an interesting mechanistic draft called the ‘gcHap-network pathway’. Meanwhile, we collected a lot of drought and salt breeding varieties, especially the introgression lines (ILs) with HHZ as the parent, which contained the above-mentioned nine genes. This might imply that these ILs have the potential to improve the tolerance to drought and salt. In this paper, we focus on the relationship of drought and salt co-response gene gcHaps and their related pathways using a novel angle. The haplotype network will be helpful to explore the desired haplotypes that can be implemented in haplotype-based breeding programs. 相似文献
95.
Jinquan Fan Binbin Li Qianming Hong Zeyu Yan Xinjun Yang Kecheng Lu Guoliang Chen Lei Wang Yihong Chen 《International journal of molecular sciences》2022,23(1)
In shrimp, several glutathione peroxidase (GPX) genes have been cloned and functionally studied. Increasing evidence suggests the genes’ involvement in white spot syndrome virus (WSSV)- or Vibrio alginolyticus-infection resistance. In the present study, a novel GXP gene (LvGPX3) was cloned in Litopenaeus vannamei. Promoter of LvGPX3 was activated by NF-E2-related factor 2. Further study showed that LvGPX3 expression was evidently accelerated by oxidative stress or WSSV or V. alginolyticus infection. Consistently, downregulated expression of LvGPX3 increased the cumulative mortality of WSSV- or V. alginolyticus-infected shrimp. Similar results occurred in shrimp suffering from oxidative stress. Moreover, LvGPX3 was important for enhancing Antimicrobial peptide (AMP) gene expression in S2 cells with lipopolysaccharide treatment. Further, knockdown of LvGPX3 expression significantly suppressed expression of AMPs, such as Penaeidins 2a, Penaeidins 3a and anti-lipopolysaccharide factor 1 in shrimp. AMPs have been proven to be engaged in shrimp WSSV- or V. alginolyticus-infection resistance; it was inferred that LvGPX3 might enhance shrimp immune response under immune challenges, such as increasing expression of AMPs. The regulation mechanism remains to be further studied. 相似文献
96.
Marta Vallverdú-Prats Ramon Brugada Mireia Alcalde 《International journal of molecular sciences》2022,23(2)
Arrhythmogenic cardiomyopathy is a heritable heart disease associated with desmosomal mutations, especially premature termination codon (PTC) variants. It is known that PTC triggers the nonsense-mediated decay (NMD) mechanism. It is also accepted that PTC in the last exon escapes NMD; however, the mechanisms involving NMD escaping in 5′-PTC, such as reinitiation of translation, are less known. The main objective of the present study is to evaluate the likelihood that desmosomal genes carrying 5′-PTC will trigger reinitiation. HL1 cell lines were edited by CRISPR/Cas9 to generate isogenic clones carrying 5′-PTC for each of the five desmosomal genes. The genomic context of the ATG in-frame in the 5′ region of desmosomal genes was evaluated by in silico predictions. The expression levels of the edited genes were assessed by Western blot and real-time PCR. Our results indicate that the 5′-PTC in PKP2, DSG2 and DSC2 acts as a null allele with no expression, whereas in the DSP and JUP gene, N-truncated protein is expressed. In concordance with this, the genomic context of the 5′-region of DSP and JUP presents an ATG in-frame with an optimal context for the reinitiation of translation. Thus, 5′-PTC triggers NMD in the PKP2, DSG2* and DSC2 genes, whereas it may escape NMD through the reinitiation of the translation in DSP and JUP genes, with no major effects on ACM-related gene expression. 相似文献
97.
Min Huang Dan Lou Qian Cai Xiuli Chang Xinjin Wang Zhijun Zhou 《International journal of molecular sciences》2014,15(10):18422-18436
Aberration during the development of the central nervous system (CNS) due to environmental factors underlies a variety of adverse developmental outcomes. Paraquat (PQ) is a widely studied neurotoxicant that perturbs the normal structure/function of adult CNS. Yet, the impacts of PQ exposure on the developing CNS remain unclear. miRNAs represent a class of small non-coding RNA molecules involved in the regulation of neural development. Thus in the present study, we analyzed the impacts of PQ on the miRNome of human neural progenitor cells (hNPCs) during proliferation by using the Exiqon miRCURY™ LNA Array. A total of 66 miRNAs were identified as differentially expressed in proliferating hNPCs upon PQ treatment. miRTarBase prediction identified 1465 mRNAs, including several genes (e.g., nestin, sox1, ngn1) previously proved to be associated with the neural proliferation and differentiation, as target genes of PQ-induced differentially expressed miRNAs. The database for annotation, visualization and integrated discovery (DAVID) bioinformatics analysis showed that target genes were enriched in regulation of cell proliferation and differentiation, cell cycle and apoptosis as well as tumor protein 53 (p53), Wnt, Notch and mitogen-activated protein kinases (MAPK) signaling pathways (p < 0.001). These findings were confirmed by real-time RT-PCR. Based on our results we conclude that PQ-induced impacts on the miRNA profiling of hNPCs undergoing proliferation may underlie the developmental neurotoxicity of PQ. 相似文献
98.
建立了中性氧化铝固相萃取柱净化,超高效液相色谱-串联质谱法(UPLC-MS/MS)测定乳和乳制品中黄曲霉毒素M1的分析方法。样品经乙腈提取,乙酸锌辅助沉淀蛋白,中性氧化铝SPE柱净化,以乙腈和0.1%甲酸水溶液作为流动相进行梯度洗脱,以电喷雾离子源(ESI)在正离子多反应监测(MRM)模式下进行测定,外标法定量。结果表明,黄曲霉毒素M1在0.1~50μg/L浓度范围内呈良好的线性关系,相关系数(r2)大于0.99;空白样品加标回收率在70.8%~79.2%之间,相对标准偏差小于8%。方法检出限为0.02μg/kg,定量限为0.05μg/kg。该方法实用、准确、灵敏,适用于乳和乳制品中黄曲霉毒素M1的测定。 相似文献
99.
以四川泡菜蔬菜原料——新鲜灯笼辣椒为对象,分析其表面附生乳酸菌Enterococcus mundtii(5 株)、Enterococcus faecalis(2 株)、Enterococcus hirae(5 株)、Lactococcus lactis(7 株)、Leuconostocmesenteroides(2 株)、Leuconostoc holzapfelii(3 株)和Weissella cibaria(79 株)对青霉素(penicillin,PEN)、红霉素(erythromycin,ERY)、四环素(tetracycline,TET)、链霉素(streptomycin,STR)和氯霉素(chloramphenicol,CHL)的抗生素耐药性和耐药基因分布,为制定合理的食品安全防控措施提供科学依据。研究表明:所有分离菌株均无PEN和ERY耐药性,其他种属部分菌株对TET、STR和CHL表现出单一、二重或三重耐药性。除E. hirae、E. faecalis和L. holzapfelii部分菌株对STR表现出单一耐药性外,所有L. mesenteroide菌株只表现出了STR单一耐药性;STR和TET、STR和CHL二重耐药菌株在E. faecalis、E. hirae、L. lactis和W. cibaria分离菌株中都有发现,但是STR、TET、CHL三重耐药菌株仅在W. cibaria中发现。聚合酶链式反应检测发现:除基因norA、sepA、tet(A)、tet(O)和aac(6’)-aph(2’)未被检出外,其他耐药菌株都有相应1 个或多个耐药基因被检出。多重耐药外排泵基因efrA、tolC、norC、sugE和mdfA较核糖体蛋白质保护和酶修饰基因检出率高,分别达到了49%、41%、48%、41%和47%。虽然辣椒表面附生乳酸菌的抗生素耐药基因在四川泡菜发酵过程中的扩散行为需要进一步研究,但根据食品加工过程安全规范标准,也应关注其表面附生的乳酸菌抗生素耐药性存在的潜在食品安全问题。 相似文献
100.