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541.
采用重氮法直接用甲胺磷连接牛血清白蛋白合成了人工抗原。通过鉴定,该人工抗原的紫外吸收光谱呈现出与半抗原、载体蛋白的紫外吸收光谱均不同的吸收峰;红外光谱图中也同时呈现了半抗原与载体蛋白的特征吸收带;并测得人工抗原中甲胺磷与载体蛋白的结合比为15∶1,表明该人工抗原合成成功。 相似文献
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大豆球蛋白与大豆β-伴球蛋白是大豆蛋白的主要组成成分,也是主要的过敏原,二者通过影响大豆蛋白的性质改变其营养和功能特性。糖基化会使大豆抗原蛋白发生不同程度的改性,从而影响大豆蛋白产品的品质与特性。因此,研究大豆抗原蛋白在糖基化过程中的结构和功能特性的变化,对促进大豆蛋白深加工具有重要意义。本文分析了大豆主要抗原蛋白组成与结构特点,综述了大豆抗原蛋白在糖基化过程中的结构变化对其功能特性的影响,具体表现在溶解性、乳化性、乳化稳定性提高,凝胶性改善、致敏性降低。这些功能特性的改变为大豆蛋白开发和应用创造了条件。其目的是为大豆蛋白糖基化机理及工业化生产提供理论。 相似文献
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Haibo Feng Yangyang Feng Lang Lin Daiyan Wu Qianqian Liu Hangyu Li Xinnan Zhang Sheng Li Feng Tang Ziwei Liu Linzi Zhang 《International journal of molecular sciences》2022,23(8)
Carbon nanotubes (CNTs) are carbon allotropes consisting of one, two, or more concentric rolled graphene layers. These can intrinsically regulate immunity by activating the innate immune system. Mannose receptors (MR), a subgroup of the C-type lectin superfamily, are abundantly expressed on macrophages and dendritic cells. These play a crucial role in identifying pathogens, presenting antigens, and maintaining internal environmental stability. Utilizing the specific recognition between mannose and antigen-presenting cells (APC) surface mannose receptors, the antigen-carrying capacity of mannose-modified CNTs can be improved. Accordingly, here, we synthesized the mannose-modified carbon nanotubes (M-MWCNT) and evaluated them as an antigen delivery system through a series of in vitro and in vivo experiments. In vitro, M-MWCNT carrying large amounts of OVA were rapidly phagocytized by macrophages and promoted macrophage proliferation to facilitate cytokines (IL-1β, IL-6) secretion. In vivo, in mice, M-MWCNT induced the maturation of dendritic cells and increased the levels of antigen-specific antibodies (IgG, IgG1, IgG2a, IgG2b), and cytokines (IFN-γ, IL-6). Taken together, M-MWCNT could induce both humoral and cellular immune responses and thereby can be utilized as an efficient antigen-targeted delivery system. 相似文献
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Lona Zeneyedpour Christoph Stingl Johan M. Kros Peter A. E. Sillevis Smitt Theo M. Luider 《International journal of molecular sciences》2022,23(9)
We investigated the feasibility of detecting the presence of specific autoantibodies against potential tumor-associated peptide antigens by enriching these antibody–peptide complexes using Melon Gel resin and mass spectrometry. Our goal was to find tumor-associated phospho-sites that trigger immunoreactions and raise autoantibodies that are detectable in plasma of glioma patients. Such immunoglobulins can potentially be used as targets in immunotherapy. To that aim, we describe a method to detect the presence of antibodies in biological samples that are specific to selected clinically relevant peptides. The method is based on the formation of antibody–peptide complexes by mixing patient plasma with a glioblastoma multiforme (GBM) derived peptide library, enrichment of antibodies and antibody–peptide complexes, the separation of peptides after they are released from immunoglobulins by molecular weight filtration and finally mass spectrometric quantification of these peptides. As proof of concept, we successfully applied the method to dinitrophenyl (DNP)-labeled α-casein peptides mixed with anti-DNP. Further, we incubated human plasma with a phospho-peptide library and conducted targeted analysis on EGFR and GFAP phospho-peptides. As a result, immunoaffinity against phospho-peptide GSHQIS[+80]LDNPDYQQDFFPK (EGFR phospho-site S1166) was detected in high-grade glioma (HGG) patient plasma but not in healthy donor plasma. For the GFAP phospho-sites selected, such immunoaffinity was not observed. 相似文献
550.
Samira Aghamiri Mojtaba Noofeli Parvaneh Saffarian Zahra Salehi Najafabadi Hamid Reza Goudarzi 《IET nanobiotechnology / IET》2022,16(5):199
This paper aims to investigate the preparation and characterisation of the alginate nanoparticles (NPs) as antigen delivery system loaded by diphtheria toxoid (DT). For this purpose, both the loading capacity (LC) and Loading efficiency (LE) of the alginate NPs burdened by DT are evaluated. Moreover, the effects of different concentrations of sodium alginate and calcium chloride on the NPs physicochemical characteristics are surveyed in addition to other physical conditions such as homogenization time and rate. To do so, the NPs are characterised using particle size and distribution, zeta potential, scanning electron microscopy, encapsulation efficiency, in vitro release study and FT‐IR spectroscopy. Subsequently, the effects of homogenization time and rate on the NPs are assessed. At the meantime, the NPs LC and efficiency in several DT concentrations are estimated. The average size of the NPs was 400.7 and 276.6 nm for unloaded and DT loaded, respectively. According to the obtained results, the zeta potential of the blank and DT loaded NPs are estimated as −23.7 mV and −21.2 mV, respectively. Whereas, the LC and LE were >80% and >90%, in that order. Furthermore, 95% of the releasing DT loaded NPs occurs at 140 h in the sustained mode without any bursting release. It can be concluded that the features of NPs such as morphology and particle size are strongly depended on the calcium chloride, sodium alginate concentrations and physicochemical conditions in the NPs formation process. In addition, appropriate concentrations of the sodium alginate and calcium ions would lead to obtaining the desirable NPs formation associated with the advantageous LE, LC (over 80%) and sustained in vitro release profile. Ultimately, the proposed NPs can be employed in vaccine formulation for the targeted delivery, controlled and slow antigen release associated with the improved antigen stability. 相似文献