Poly(brilliant cresyl blue) electrogenerated on single-walled carbon nanotubes modified electrode and its application in mediated biosensing system

Single-walled carbon nanotubes (SWCNTs) functionalized with carboxylic acid groups were cast to glassy carbon electrode (GCE) to construct a three-dimensional nano-micro structured scaffold. Brilliant cresyl blue (BCB) was electropolymerized on the above-mentioned SWCNTs/GCE using continuous cycling between −0.7 and 0.9 V vs. SCE. PolyBCB yielded on SWCNTs/GCE exhibited the enhanced electrochemical redox behavior compared with that electrogenerated on bare GCE. The apparent surface coverage of PolyBCB obtained by SWCNTs/GCE was at least 10 times higher than that obtained by bare GCE, namely 4.8 × 10⁻⁹ and 3.6 × 10⁻¹⁰ mol cm⁻². The cyclic voltammograms recorded by PolyBCB/SWCNTs/GCE exhibited well-defined two peaks located at −0.25 V and −0.06 V, respectively, with a surface-controlled mechanism. In addition, morphologies of PolyBCB electrogenerated on GCE and SWCNTS/GCE were characterized by atomic force microscopy. Finally, this proposed PolyBCB/SWCNTs/GCE was used in the construction of the second-generation biosensors to hydrogen peroxide and glucose, with the enhanced analytical performance.     

Label-free electrochemical immunosensor for sensitive detection of kanamycin

A novel label-free electrochemical immunosensor for sensitive detection of kanamycin based on water-soluble graphene sheet (WGS)/prussian blue-chitosan (PB-CTS)/nanoporous gold (NPG) composited film has been reported. PB was selected as an electron transfer mediator, and was modified onto the electrode together with WGS through electrostatic adsorption. Then NPG was immobilized onto the as-prepared film for biomolecules anchoring. The electroactivity of PB was greatly enhanced in the presence of WGS and NPG. It could mainly be ascribed to the fact that the good conductivity of WGS and NPG promoted electron transfer and enhanced the sensitivity. kanamycin antibody, as a model, was immobilized onto the composite film for the detection of kanamycin. Under optimum conditions, the amperometric signal of PB decreased linearly with kanamycin concentration (0.02-14 ng mL⁻¹), a linear calibration plot (y = 1.3817 + 4.7544x, r = 0.9993), resulting in a low limit of detection (6.31 pg mL⁻¹). The novel immunosensor for the detection of kanamycin in real sample with satisfactory results has been proved. In addition, this method would be easily adapted for the detection of other residual antibiotics in animal derived foods.     

A dual K-Na selective Prussian blue nanotubes sensor

A strategy for dual sensing of Na⁺ and K⁺ ions using Prussian blue nanotubes via selective inter/deintercalation of K⁺ ion and competitive inhibition by Na⁺ ion, is reported. The analytical signal is derived from the cyclic voltammetry cathodic peak position E_pc of Prussian blue nanotubes. Na⁺ and K⁺ levels in a sample solution can be determined conveniently using one Prussian blue nanotubes sensor. In addition, this versatile method can be applied for the analysis of single type of either Na⁺ or K⁺ ions. The dual-ion sensor response towards Na⁺ and K⁺ can be described using a model based on the competitive inhibition effects of Na⁺ on K⁺ inter/deintercalation in Prussian blue nanotubes. Successful application of the Prussian blue nanotubes sensor for Na⁺ and K⁺ determination is demonstrated in artificial saliva.     

改进的Knockout自然景物提取算法

针对Knockout算法中颜色估计模型只适用于光滑图像的问题,提出一种新的颜色估计模型。采用区域生长法将未知区域划分为若干个子区域,以距离每一个子区域最近的前景和背景轮廓线上的点为中心做矩形块,分别计算2个矩形块内前景和背景像素点的颜色加权平均值,将其作为该子区域的前景和背景颜色分量。实验结果表明,改进后的颜色估计模型在图像边缘附近颜色相差较大的自然景物提取中有较好表现。     

基于NaOH刻蚀玻碳电极的电化学传感器在检测膀胱癌DNA中的应用

制备了一种基于活化的玻碳电极的新型电化学DNA生物传感器,可用于膀胱癌DNA的检测.通过循环伏安法(CV)实现玻碳电极在NaOH溶液中的刻蚀,使电极表面负载大量官能团,为DNA提供连接位点,由Laviron方程计算得到玻碳电极表面的羧基浓度为 1.022×10-6 mol/cm2.亚甲基蓝(MB)作为电化学检测的杂交指示剂.采用原子力显微镜(AFM)对刻蚀后的电极进行了形貌表征.在最优杂交条件下,通过差分脉冲法(DPV)计算出最佳检测限为5.677×10-13 mol/L(n=5),适用目标 DNA浓度范围1×10-8 mol/L~1×10-12 mol/L.该传感器有望用于实际样品中膀胱癌DNA的快速检测.     

纳米金-壳聚糖-血红蛋白/普鲁士蓝/金电极检测过氧化氢

在普鲁士蓝修饰的金电极上,通过纳米金/壳聚糖复合膜固定血红蛋白,研制了安培型过氧化氢生物传感器,线性范围和检测限分别为1～5 000 umol/L和0.27umol/L.     

锂盐改性累托石处理染料废水

采用锂盐对累托石进行改性,研究其最佳改性条件及吸附亚甲基蓝的热力学和动力学规律.以碳酸锂为改性剂,碳酸锂与草酸用量比为0.8∶1,试验出改性的最佳条件为：碳酸锂与累托石质量比为5%,固液比为1∶20,改性时间为3 h.改性后累托石对亚甲基蓝溶液的吸附性能明显优于未改性的累托石,吸附量提高了65%;热力学和动力学探究表明,改性累托石对亚甲基蓝的吸附过程符合Langmuir规律,动力学符合Lagergren准二级动力学规律.     

电子蓝军装备综合演练运筹分析及其平行系统构建

阐述了电子蓝军装备综合演练自身训练和对抗应用的2种模式。从电子蓝军建设论证、综合演练需求分析、综合演练方案优化、导调与指挥决策辅助、综合演练效果评估5个方面分析了电子蓝军装备综合演练运筹分析要解决的主要问题及其特点。提出了基于ACP方法的电子蓝军装备综合演练运筹分析框架,初步构建了电子蓝军装备综合演练运筹分析平行系统。运筹分析平行系统的提出,为电子蓝军装备综合演练总体设计、组织实施和评估研究提供了新的手段。     

普鲁士蓝对Rb＋、Cs＋的吸附性能研究

摘要： 用K4Fe（CN）6·3H2O和FeCl3·6H2O制备纯普鲁士蓝（PB）和负载量为55％普鲁士蓝/二氧化硅（PB/SiO2）,考察了不同因素条件下（吸附时间、起始离子含量、c（H＋）浓度、干扰阳离子K＋和NH4＋）,纯PB对Rb＋、Cs＋混合模拟液的静态吸附情况.结果表明：随着吸附时间的增加,Kd（Rb＋）和Kd（Cs＋）增大,随着起始离子含量、c（H＋）浓度、干扰阳离子K＋和NH4＋浓度的增大,Kd（Rb＋）和Kd（Cs＋）整体来说稍有下降,但变化不大.由于纯PB在水中易分散,不利于柱操作,故选取负载量为55％ PB/SiO2进行柱实验,结果表明此柱对Rb＋、Cs＋显示出好的离子交换性能.     

用磷酸活化法制备甘蔗渣活性炭及其吸附性能研究

以甘蔗渣为原料,在不同操作条件下制备得到活性炭,测定了相应的活性炭对亚甲基蓝脱色的吸附值,并研究了亚甲基蓝吸附值与活化剂浓度、活化时间和活化温度之间的关系.实验结果表明,浸渍剂浓度是用磷酸活化法制备活性炭的最重要的影响因素;在磷酸浓度为40 wt％,活化时间为12 h,活化温度为500℃时,甘蔗渣活性炭的吸附能力最高,亚甲基蓝的吸附量达到294.866 mg/g.