用强度系列成象法获取或显示高动态范围图象

提出一种适合于高动态范围图象的获取和显示的强度系列成象法。改变输入图象的强度，微机控制的图象采集系统进行记录得到一组磁盘文件，形成强度图象系列。基于记录的系列图象，可以进行信息提取、变换和显示等操作。基于获得的图象系列，提出一种剪贴操作进行快速的非线性图象变换。给出对光学功率谱处理的一个实例，它相当于进行锯齿形的非线性变换。     

~（131）Ⅰ—MIBG比活度对鼠心肌交感神经囊泡内外摄取的影响

研究了两种比活度的１３１Ⅰ－ＭＩＢＧ在正常及利血平抑制后在鼠体内的分布，结果表明：注射后４ｈ，正常鼠心肌对３．２２ＴＢｑ／ｇ的ＭＩＢＧ摄取为９．０８％ＩＤ／ｇ，明显高于对１８．５ＧＢｑ／ｇ的摄取（４．８７％ＩＤ／ｇ）。计算表明，比活度主要影响了鼠心肌交感神经囊泡内摄取，而与囊泡外摄取无关．当ＭＩＢＧ用于心肌显像时，高比活度的ＭＩＢＧ有利于诊断。     

论反射地震偏移成像

对反射地震偏移成像问题进行了分析、对比与探讨，并对各种偏移方法如叠后时间偏移、叠后深度偏移、叠前时间偏移、叠前深度偏移和它们之间在地质效果上的异同进行了讨论。认为，在当前的地面观测记录的质量条件下，叠后时间偏移和叠后深度偏移，叠前时间偏移和叠前深度偏移，都有各自的优势和不足。因此，它们都有应用价值，不是互相排斥，而是互相补充。今后的方向是发展和完善高分辨率和高保真度的偏移成像方法。在这方面还有许多问题要解决。     

N_2S_2或N_3S型配体的合成、~(99)Tc~m标记及生物分布研究

以MAG3为基本分子骨架，根据构效关系，分别引入合适的天然氨基酸，设计合成了4种N2S2或N3S型小分子多肽新配体，并通过了IR，^1H NMR,^13C NMR,MS谱学鉴定和元素分析表征。采用葡庚糖酸钙（GH）交换法对4个配体进行了^99Tc^m标记，研究了配合物在小鼠体内的生物分布特征。结果表明，^99Tc^m-MVGG肾摄取较高，滞留时间较长，血清除快，且肾与其它组织的活度比值高，具备成为肾功能显像剂的条件；^99Tc^m-MPGG肾初始摄取较高，R（肾／血）活度比值高，但肾清除较快，R（肾／肝）活度比值较低；^99Tc^m-MVTC和^99Tc^m－MPTC心肌初始摄取均较高，但在心肌和血中的清除速度较快。     

Signal analysis of total internal reflection fluorescent speckle microscopy (TIR-FSM) and wide-field epi-fluorescence FSM of the actin cytoskeleton and focal adhesions in living cells

Fluorescent speckle microscopy (FSM) uses low levels of fluorescent proteins to create fluorescent speckles on cytoskeletal polymers in high‐resolution fluorescence images of living cells. The dynamics of speckles over time encode subunit turnover and motion of the cytoskeletal polymers. We sought to improve on current FSM technology by first expanding it to study the dynamics of a non‐polymeric macromolecular assembly, using focal adhesions as a test case, and second, to exploit for FSM the high contrast afforded by total internal reflection fluorescence microscopy (TIR‐FM). Here, we first demonstrate that low levels of expression of a green fluorescent protein (GFP) conjugate of the focal adhesion protein, vinculin, results in clusters of fluorescent vinculin speckles on the ventral cell surface, which by immunofluorescence labelling of total vinculin correspond to sparse labelling of dense focal adhesion structures. This demonstrates that the FSM principle can be applied to study focal adhesions. We then use both GFP‐vinculin expression and microinjected fluorescently labelled purified actin to compare quantitatively the speckle signal in FSM images of focal adhesions and the actin cytoskeleton in living cells by TIR‐FM and wide‐field epifluorescence microscopy. We use quantitative FSM image analysis software to define two new parameters for analysing FSM signal features that we can extract automatically: speckle modulation and speckle detectability. Our analysis shows that TIR‐FSM affords major improvements in these parameters compared with wide‐field epifluorescence FSM. Finally, we find that use of a crippled eukaryotic expression promoter for driving low‐level GFP‐fusion protein expression is a useful tool for FSM imaging. When used in time‐lapse mode, TIR‐FSM of actin and GFP‐conjugated focal adhesion proteins will allow quantification of molecular dynamics within interesting macromolecular assemblies at the ventral surface of living cells.     

Evaluation of image similarity by histogram intersection

Colour is the most widely used attribute in image retrieval and object recognition. A technique known as histogram intersection has been widely studied and is considered to be effective for color‐image indexing. The key issue of this algorithm is the selection of an appropriate color space and optimal quantization of the selected color space. The goal of this article is to measure the model performance in predicting human judgment in similarity measurement for various images, to explore the capability of the model with a wide set of color spaces, and to find the optimal quantization of the selected color spaces. Six color spaces and twelve quantization levels are involved in evaluating the performance of histogram intersection. The categorical judgment and rank order experiments were conducted to measure image similarity. The CIELAB color space was found to perform at least as good as or better than the other color spaces tested, and the ability to predict image similarity increased with the number of bins used in the histograms, for up to 512 bins (8 per channel). With more than 512 bins, further improvement was negligible for the image datasets used in this study. © 2005 Wiley Periodicals, Inc. Col Res Appl, 30, 265–274, 2005; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/col.20122     

Immunoelectron localization of mitochondrial F1 factor in cryosections of heart muscle cells

Immunocytochemistry was used to investigate the localization of F1 ATPase in mitochondria of cryosections of adult mouse heart muscle cells. The initial aldehyde fixation was the only denaturation step for antigens. The fine structure was preserved with contrast enhancement as the sections were maintained hydrated, with the advantage that the entire procedure is completed in one working day. The reaction was highly specific, and entire mitochondria were labeled with the Protein A-gold complex. A new analytical technique, electron spectroscopic imaging (ESI), contributed to a better visualization of the localization of the F1 factor.     

WKB近似下的Fourier衍射成象方法

对于介质目标微波衍射成象，本文引入了ＷＫＢ近似来模拟目标内部总场。基于这种近似，我们导出了Ｆｏｕｒｉｅｒ衍射公式，并采用了广义滤波逆传播方法由目标空间谱实现目标特性的重建。计算机模拟结果表明采用ＷＫＢ近似重建目标特性较Ｂｏｒｎ近似有明显改善。     

Sirius叠前深度偏移在莺歌海盆地“泥拱模糊带”处理中的应用

在对Sirius叠前深度偏移软件应用开发的基础上,分析讨论了Sirius 2D叠前深度偏移速度模型建立、层析成像及演、叠前深度偏移以及深度聚焦等技术环节的基本原理,并以莺歌海盆地某测线的处理为例,具体分析了Sirius 2D叠前深度偏移处理的流程、主要参数及时效.文中还分析讨论了莺歌海盆地中央“泥拱带”叠前深度偏移处理的地质效果.实践表明,莺歌海盆地广泛发育的与“泥拱模糊带”有关的构造各有其特殊性,只有通过3D叠前深度偏移处理才能从根本上解决“泥拱模糊带”的成像问题.     

Progress in deep-UV photoresists

Higher resolution can be achieved in lithography by decreasing the wavelength of the exposure source. However, resist material and their processing are also important when we move to a shorter wavelength lithography technology. This paper reviews the recent development and challenges of deep-UV photoresists and their processing technology.