Radio-resistance induced by nitric oxide to heavy ion irradiation in A172 human glioma cells

To investigate effects of nitric oxide on cellular radio-sensitivity, three human glioma cell lines, i.e. A172, A172 transfected green fluorescence protein (EGFP) gene (EA172) and A172 transfected inducible nitric oxide synthesis (iNOS) gene (iA172), were irradiated by 12C6 ions to 0, 1 or 2Gy. Productions of nitric oxide and glutathione (GSH) in A172, EA172 and iA172 were determined by chemical methods, cell cycle was analyzed by flow cytometry at the 24th hour after irradiation, and survival fraction of the cells was measured by colorimetric MTT assay at the 5th day after irradiation. The results showed that the concentrations of nitric oxide and GSH in iA172 were significantly higher than in A172 and EA172; the G2/M stage arrest induced by the 12C6 ion irradiation was observed in A172 and EA172 but not in iA172 at the 24th hour after exposure; and the survival fraction of iA172 was higher than that of EA172 and iA172. Data suggest that the radio-sensitivity of the A172 was reduced after the iNOS gene transfection. The increase of GSH production and the change of cellular signals such as the cell cycle control induced by nitric oxide may be involved in this radio-resistance.     

基于光纤光栅技术黏性土中静压闭口管桩试验研究

为研究黏性土中静压闭口管桩的贯入机理,开展了室内模型试验.制作了两根不同直径的闭口管桩,通过桩身开浅槽,单根嵌入6个增敏微型光纤光栅传感器,连续监测了静力压桩过程桩身内力变化规律,试验结果表明:由于FBG传感技术灵敏系数高、长期稳定性好等优点,因此能较好的监测沉桩过程桩身受力状态.桩径越大,摩阻比、单位摩阻力越大,端阻...     

城市有效不透水下垫面及其识别研究进展

城市中不透水下垫面,尤其是有效不透水下垫面的增加,会对城市水文循环的各个环节产生影响。针对城市有效不透水下垫面及其识别的问题,剖析了不透水下垫面的水文效应,从水文学的角度辨析了采用总不透水面积和有效不透水面积衡量城市不透水性的优缺点,系统梳理了现场调查与测定法、遥感及影像资料分析法、土地利用类型确定法、经验公式法以及降雨-径流关系法等国内外常用的有效不透水下垫面识别方法的优点与不足,指出未来需深入分析城市不同种类不透水下垫面之间的关系及其对城市雨洪过程的影响,从而提高城市雨洪模拟精度,为城市洪涝防治和海绵城市建设提供技术支撑。     

湿污泥颗粒的流化床干燥实验及模型

在鼓泡流化床内以河砂为干燥介质,对单颗粒湿污泥的流态化干燥特性进行实验研究,得到了流化床温度、污泥初始水分、污泥粒径及流化速度对干燥速率的影响规律：流化床温度及污泥粒径对干燥速率的影响都呈指数规律;污泥的水分越大,干燥速率越大;在鼓泡流化床流化速度达到2倍临界流化速度以上时,充分流化,流化速度再增大(2~5倍临界流化速度)对干燥速率没有明显影响. 在基本的扩散传质理论的基础上,利用实验数据回归得到湿污泥在鼓泡流化床内干燥的半经验模型,为流化床污泥干燥器的设计提供了基础数据和依据.     

一类具隔离项的动态SEIS模型的定性分析

建立了一类具隔离项和人口动态变化的新的SEIS模型,得到了平衡点的渐近稳定条件,讨论了系统的Hopf分岔和极限环.     

A Novel Role of Arrhythmia-Related Gene KCNQ1 Revealed by Multi-Omic Analysis: Theragnostic Value and Potential Mechanisms in Lung Adenocarcinoma

The early diagnosis, prognostic prediction, and personalized therapy of lung adenocarcinoma (LUAD) remains a challenging issue. KCNQ1 (potassium voltage-gated channel subfamily Q Member 1) is implicated in long QT syndrome (LQTS) and cardiac arrhythmia, while its significance in LUAD remains unclear. In this study, we aimed to explore the significance of KCNQ1 in terms of clinical value, tumor immunity, underlying mechanisms, and a precision medicine approach by means of multi-omics analysis. The association of KCNQ1 with LUAD was first explored. Both altered variants and high expression of KCNQ1 in a TCGA-LUAD cohort indicated a favorable outcome. KCNQ1 levels had a negative correlation with tumor proliferation index Ki67 levels. siRNA-knockdown of KCNQ1 promoted the migration ability of lung cancer cells. KCNQ1 levels were decreased in LUAD tissue compared to normal tissue. A receiver operating characteristic (ROC) curve indicated good diagnostic efficiency of KCNQ1. High KCNQ1 is associated with an immunoactive profile of immune infiltration and immunomodulators and is involved in the inhibition of the cell cycle and DNA replication. Lapatinib was identified as a potent drug for LUAD in the context of low KCNQ1. This study unveiled the significance of KCNQ1 in diagnosis and prognosis and provided a corresponding precision medicine strategy for LUAD.     

聚丙烯纤维网混凝土桥面抗冻性探讨

以聚丙烯纤维网水泥混凝土替代普通水泥混凝土作为桥面铺装材料,可以改善混凝土抗冻性能,并能够利用聚丙烯纤维网良好的性能减轻桥面铺装混凝土在低温作用下的破坏,增加行车的舒适性,延长铺装及桥梁主体的使用寿命。本文对聚丙烯纤维网水泥混凝土桥面铺装的抗冻耐久性进行了试验研究,并对试验结果加以分析。     

水泥稳定砂砾基层冰冻耐久性试验分析

针对黑龙江省寒冷的气候特点,通过室内试验(利用低温实验室模拟自然环境进行冻融循环试验),分析比较不同级配的水泥稳定砂砾冰冻作用后强度衰减规律,获得抵抗冻融破坏作用的级配范围及最优配合比,为冻融循环作用下的基层设计提供有一定可靠度的基层强度设计值,保证基层在使用周期内具有一定的冰冻耐久性,从而可以提高道路使用质量和延长道路使用寿命,降低维修养护费用。     

基于曲波变换和快速非局部均值的地震数据随机噪声压制

在常规的地震勘探中,由于受到激发、接收环境或仪器等因素影响,地震数据中会不可避免地混杂随机噪声,导致有效信号被淹没,从而无法被清晰识别,因此随机噪声的压制至关重要.本次研究提出一种改进曲波变换阈值去噪结合快速非局部均值去除地震随机噪声的方法:首先将循环平移和块状复数域阈值方法引入到曲波变换阈值去噪中,改进传统的曲波阈值...     

不可逆回热式玻色布雷顿制冷循环性能分析

基于玻色气体的热力学性质和回热式布雷顿制冷循环的不可逆模型,导出循环的一些重要性能参数,如循环的制冷量、回热量、输入功和性能系数的一般表达式。通过数值计算获得了循环的一些重要的性能特性曲线,分析了循环的不可逆性和回热特性对玻色布雷顿制冷机性能的影响。