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21.
S. MESCHINI A. MOLINARI A. CALCABRINI G. CITRO G. ARANCIA 《Journal of microscopy》1994,176(3):204-210
The intracellular distribution of the anthracyclinic antibiotic adriamycin in living cultured cells has been investigated by confocal microscopy. In human melanoma cells (M14), adriamycin was localized inside the nuclei. When adriamycin-treated M14 cells were allowed to recover in drug-free medium, a complete efflux of the drug from the nucleus was revealed. In recovered cells, a weakly fluorescent signal was observed in the perinuclear region. When M14 cells were recovered in a medium containing colcemid, a microtubule depolymerizing agent, the drug transport from the nucleus to the cell periphery appeared to be inhibited, suggesting that the microtubule network is strongly involved in drug transport mechanisms. In multidrug-resistant (MDR) cells the intracellular location of adriamycin was shown to be noticeably different from that of the parental wild-type cells. In particular, in resistant human breast carcinoma cells (MCF-7), adriamycin appeared to be exclusively located within the cytoplasm whereas the nuclei were shown to be completely negative. When adriamycin treatment was performed in association with MDR revertants, such as Lonidamine (inhibitor of the energy metabolism) or verapamil (inhibitor of the P-glycoprotein efflux pump), a marked enhancement of the cytoplasmic signal was observed in resistant cells. Under these conditions, adriamycin appeared concentrated in the perinuclear region, but the nuclei were still negative. Confocal microscopy proved to be a very useful method for the study of the intracellular transport of fluorescent substances, such as anthracyclinic antibiotics, and for the investigation of the multidrug resistance phenomenon in tumour cells. 相似文献
22.
核电材料在模拟反应堆环境中应力腐蚀破裂测试技术与性能评价 总被引:1,自引:0,他引:1
杨武 《理化检验(物理分册)》1996,32(5):7-12
简要介绍了采用慢应变速率试验,U型弯曲和C形环试验等技术,分别对800合金,304和316及316Ti不锈钢A533B压力容器用多在模拟核反应堆环境中的应力腐蚀破裂敏感性性进行的试验研究的一些主要结果;并结合电化学测试和表面膜俄歇电子能谱分析结果进行了讨论。 相似文献
23.
湿硫化氢环境下的球罐腐蚀状况分析 总被引:5,自引:0,他引:5
在湿硫化氢环境下 ,尤其储存介质中的硫化氢含量超标时 ,很容易对储存容器壳体 (含焊缝和母材 )造成硫化氢应力腐蚀或氢鼓包。作者较仔细分析了 1 0 0 0m3 丙烯球罐产生硫化氢应力腐蚀开裂和40 0m3 LPG球罐产生氢鼓包的原因。提出了此类设备在设计选材、设备制造、施工安装和使用维护等环节应注意的问题。 相似文献
24.
激光熔覆Ni基SiC合金涂层组织与性能的研究 总被引:5,自引:0,他引:5
利用5kWCO2连续波激光器在16Mn钢基材表面对含20%(体积比)SiC陶瓷粉末的镍基自熔性合金粉末进行激光熔覆得到Ni基SiC合金涂层(NiSiC)。研究了合金涂层的组织形貌及相结构,并用单纯的镍基合金涂层(Ni60)进行了显微硬度及滑动磨损性能的对比试验。结果表明,NiSiC合金涂层由γ枝晶及其间的共晶组织组成,主要组成相为γ-Ni,γ-(Ni,Fe)固溶体和(Cr,Fe)7C3,Cr23C6及(Cr,Si)3Ni3Si等化合物。添加SiC的镍基合金涂层NiSiC比单纯的镍基合金涂层Ni60具有较高的硬度和耐磨性。 相似文献
25.
26.
Thin films on aluminum-tungsten alloys were prepared by co-deposition of pure aluminum and pure tungsten, each sputtered by an independently controlled magnetron source, on glass and sapphire substrates. Completely amorphous films were obtained in the Al80W20-Al67W33 composition range. Passivity and corrosion behavior of amorphous Al-W alloys were investigated in 1 M deaerated hydrochloric acid solution using polarization and impedance spectroscopy measurements and have been correlated with the properties of pure alloy components. Tungsten and sputter-deposited Al-W thin films are inherently passive materials while aluminum undergoes pitting corrosion in hydrochloric acid solution. The passive film formed at the OCP on each alloy possesses excellent electric and dielectric properties comparable to those of the isolating film on tungsten. The absolute impedance increases with increasing tungsten content in the alloy. According to electrochemical polarization measurements, alloying Al with W in solid solution significantly enhances the material's resistance to pitting corrosion by shifting the breakdown potential above 2000 mV (Al67W33) and lowering the corrosion rate at the OCP by more than two orders of magnitude. The most likely mechanism explaining the passivity of amorphous Al-W alloys, the Solute Vacancy Interaction Model (SVIM), involves the formation of complexes between highly oxidized solute atoms (W+6) and mobile cation vacancies, which restrict the transport of Cl− through the oxide film and inhibit its breakdown in hydrochloric acid solution. The role that film stress relaxation effects and microscopic defects in amorphous Al-W films, of the some composition, and deposited on various substrates play in their corrosion resistance is discussed. 相似文献
27.
提高自蔓延陶瓷内衬钢管耐蚀性研究的进展 总被引:1,自引:0,他引:1
广泛应用于石油,化工,能源等领域的钢管,除了要有高强度外,还要有良好的耐蚀性。自蔓延高温合成技术是制备耐蚀钢管的新技术,具有工艺简单,成本低和产品耐蚀性能好等特点。介绍了自蔓延高温合成陶瓷内衬钢管的原理和提高陶瓷内衬钢管耐蚀性的措施,展望了耐蚀陶瓷内衬钢管在工业生产上的应用前景。 相似文献
28.
Ramazan Kahraman 《Journal of Materials Engineering and Performance》2002,11(1):46-50
The objective of this study was to evaluate the effectiveness of sodium benzoate as an inhibitor to slow down or prevent atmospheric
corrosion/discoloration of the local mild steel during storage in the Arabian Gulf region. Test specimens were prepared from
locally produced reinforcing steel products. The inhibitor solution was applied on steel specimens at a concentration of 100
mM for 1 day at room temperature. Wooden exposure racks were used to hold as-received and inhibitor-treated specimens during
atmospheric exposure for different periods. Corrosion was evaluated through weight loss determination and electrochemical
technique. As expected, the Arabian Gulf atmosphere was corrosive on the as-received local mild steel. On the other hand,
treatment of steel with sodium benzoate lowered its corrosion rate during initial days of its exposure to atmosphere. However,
atmospheric corrosion inhibition performance of sodium benzoate deteriorated with exposure time after 30 or more days of atmospheric
exposure, and the corrosion rates of sodium benzoate-treated specimens reached that of the unprotected specimens at the end
of 90 days of atmospheric exposure. 相似文献
29.
在介绍“铝丝印”概念的基础上,从金属腐蚀学的观点出发,分析了“铝丝印”形成的电化学腐蚀机理,并提出了具体的解决措施。 相似文献
30.
以灌溉、防洪为主的峡口水库大坝,为沥青混凝土面板堆石坝,为研究低温抗裂性能,对沥青混凝土面板破损取样,进行了配比和多种力学性能试验,并与原面板设计指标进行了对比。由于面板经受住了-20℃以下的低温考验,其所用沥青指标、破损取样试验结果,特别是冻断低温指标,对确定水工沥青混凝土低温抗裂检验标准有重要参考价值。 相似文献