Effect of temperature on recombinant protein production using the Bm5/Bm5.NPV expression system

A series of experiments have been conducted using a recombinant baculovirus/insect cell expression system (Bm5/Bm5.NPV.CAT) to establish the optimum temperature for both cell growth and virus infection. Bm5 cell growth was found to be limited at temperatures below 22°C and ceased completely at temperatures above 34°C. In the range between 24 and 28°C, final cell densities always reached 96% of the highest achievable viable cell density. The shortest population doubling time was obtained at 28°C. Overall, a consistent increase in metabolism with increasing temperatures was observed. During the infection/viral replication phase, an increase in the temperature from 25 to 31°C resulted in a faster decrease in viable cell density and an earlier production of chloramphenicol acetyltransferase (CAT). Furthermore, protein yield at temperatures above 28°C was significantly reduced. Overall, the best temperature for the infection phase for the Bm5/Bm5.NPV expression system was found to be 25°C when the cells are cultured in serum free media.     

Powers of observation in creative design

Being perceptive is a trait highly valued in scientific and engineering professions. What a scientist or engineer notices while considering a problem, evaluating alternatives, or interpreting data has a profound impact on how a problem is viewed and solved. This paper focuses on processes we believe underlie being perceptive: firstly, preparation—becoming attuned to salient or important features; secondly, assimilation-detection and exploration of patterns (invariants) as well as anomalies; and thirdly, strategic control-heuristic strategies for exploring the implications of what has been observed. These processes play an integral role in characteristic activities within creative design, including problem reformulation, the emergence of properties and constraints on the solution, and the ability to incorporate into the design experimental feedback from the environment and from experiences with prototypes and previous designs. The paper presents a computational model incorporating these ideas, implemented in a system called IMPROVISER.     

The role of forest and bioenergy strategies in the global carbon cycle

Forest and bioenergy strategies offer the prospect of reduced CO₂ emissions to the atmosphere. Such strategies can affect the net flux of carbon to the atmosphere through 4 mechanisms: storage of C in the biosphere; storage of C in forest products; use of biofuels to displace fossil-fuel use; use of wood products which often displaces other products that require more fossil fuel for their production. We use the mathematical model GORCAM (Graz/Oak Ridge Carbon Accounting Model) to examine these mechanisms for 16 land-use scenarios. Over long time intervals the amount of C stored in the biosphere and in forest products reaches a steady state and continuing mitigation of C emissions depends on the extent to which fossil fuel use is displaced by the use of bioenergy and wood products. The relative effectiveness of alternative forest and bioenergy strategies and their impact on net C emissions strongly depend, for example, on the productivity of the site, its current usage, and the efficiency with which the harvest is used. When growth rates are high and harvest is used efficiently, the dominant opportunity for net reduction in C emissions is seen to be fossil-fuel displacement. At the growth rates and efficiencies of harvest utilization adopted in many of our base scenarios, the net C balance at the end of 100 years is very similar whether trees are harvested and used for energy and traditional forest products, or reforestation and forest protection strategies are implemented. The C balance on a plantation system that provides a constant output of biomass products can look different than the balance of a single parcel of land.     

“中国式”非显形奢华

奢侈品在中国逐渐成为热门产业,建立在精神层面上的“非显形奢华”也渐为众人关注。中国拥有深厚的文化底蕴,足以创造撼动视觉新体验的新“奢华”美学。当创意市场不断推进,变得成熟之后,中国的“非显形奢华”定会有更高的价值,奢华不再是炫耀身份的代名词,而是融合民族特色、具有独特的设计品位,真正意义上的顶级产品或服务,是一种低调、内敛、优雅的“非显形奢华”。     

全球化进程中建筑的趋同

对全球化进程中建筑趋同的原因进行了分析,探讨了全球化对中国建筑的影响,并对我国当代建筑创作问题提出了见解,以使人们对建筑全球化的趋同有一个较为清晰的认识。     

明清图书翻译的特征、种类及作用

本文对我国在明末和清代西学东渐过程中的图书翻译进行了梳理和剖析,总结了这一时期西学翻译的历史概况,阐述了东西文化交流中翻译图书的重要作用,以及历史上有识之士对于西学翻译的贡献。     

ALGORITHMIC SYNTHESIS OF GENERAL NON-SHARP SIMPLE DISTILLATION COLUMN SEQUENCES

An algorithmic approach was used to select recoveries for non-sharp sequence designs. Simple distillation columns were employed. The non-key component distribution was modeled using the Fenske equation. The combination of producing impure products and allowing non-key component distribution, results in a large search space. With this large search space size and software limitations, five problems were solved (see summary table in the Design Examples sub-section). The optimal non-key component distribution was found to be significant but not necessarily maximal. Parallel processing was selected as optimal in contrast to the more traditional sequential processing.     

提高热轧带卷的成材率途径

莱钢1500mm热轧生产线于2005年6月投产。通过对轧钢生产过程中各环节的有效控制,使成材率显著提高,居于全国同行业领先水平,取得了良好的经济效益。     

晋煤集团赵庄煤矿建筑外部空间规划浅谈

通过对晋煤集团赵庄煤矿建筑外部空间规划的实例分析,综合考虑工业、行政、生活区的不同功能,构建园林、人文、旅游式矿山,从文化提炼、总体把握、景观构成等方面解决了煤矿建设中的可持续发展问题,以达到良好的规划效果。     

石膏制品防水防潮剂的研制与开发

本文提出采用高分子有机材料配合，生产石膏制品防水防潮剂的生产方法和工艺。该剂还可以应用到氧化镁系列板材，同时提出开发珠光或彩色防水防潮剂，能达到满足的装饰效果，值提开发推广。