20世纪60年代美国青年对抗文化初探

青年对抗文化是20世纪60年代美国社会的一大特色。繁荣的经济，如火如荼的黑人民权运动，“越战”和“新左”思想是对抗文化产生的重要因素。消极的嬉皮士文化和积极的青年学生运动是60年代对抗文化的主要形式。相同的目标，使这两股力量在必要的时候联合起来。他们的努力取得一定成果，成为牵制美国对越战争的主要力量，但是随着60年代末，70年代初美国经济的衰退和右倾势力的强大，学生运动和嬉皮士文化都走向衰落。     

论正确把握社会主义文化主旋律

发展先进文化必须正确把握社会主义文化主旋律,坚持马克思主义在意识形态的指导地位,用"三个代表"统领文化建设,坚持和贯彻党的文化发展方针,正确处理文化发展的多重关系.     

大学语文文化育人新思考

大学语文要实践文化育人的理念，就要把握文化导向，坚持民族文化的主导地位，创新教学的思维理念，创新教学方法，将大学语文文本所蕴涵的丰富的“文化源”借助课程平台转化为学生个体的精神财富，培养学生文化传承的自觉意识。     

用富集培养技术分离具有PAL活性的酵母菌

为获取具有ＰＡＬ活性的菌株，设计了从天然源分离酵母菌的定集培养技术，并从中国西南地区采集２０个样品中得到菌株１４０余株，结果表明本富集培养技术用于从天然源分离具有苯丙氨酸解氨酶活性的菌株是有效、可行的。     

经济全球化与中国传统文化

经济全球化已成为当今世界经济发展的趋势。经济全球化给中国经济的发展带来了许多有利条件，与此同时，也对中国文化，特别是传统文化带来了很大冲击和影响。我们应当采取科学合理的对策，使中国传统文化焕发出新的生命力。     

客家土楼中的象征文化浅析

客家土楼以其鲜明的风格成为世界建筑艺术的一朵奇葩,其中蕴含着丰富的象征文化.试从象征的源起及在建筑中的具体体现等方面,对客家土楼中的象征文化进行诠释.通过研究其中的象征文化,有助于更完整地认识客家土楼建筑文化的全部涵义.     

当代信息传播设计的趋势和走向

通过信息社会人类对信息感知的扩展性研究 ,从信息感知方式及思维形态的差异性来分析传统印刷文化与当代视屏文化的异同 ,以图形语言的变化来揭示当代视觉传播设计技术特征、视觉特征、多元化语言特征以及发展趋势     

Discriminative Stimulus, Reinforcing, Physical Dependence, and Antinociceptive Effects of Oxycodone in Mice, Rats, and Rhesus Monkeys.

Despite oxycodone's (4,5-epoxy-14-hydroxy-3-methoxy-17-methylmorphinan-6-one) history of clinical use and the attention it has received as a drug of abuse, few reports have documented its pharmacology's relevance to its abuse or its mechanism of action. The purposes of the present study were to further characterize the analgesic effects of oxycodone, its mechanism of action, and its effects in terms of its relevance to its abuse liability. The results indicate that oxycodone had potent antinociceptive effects in the mouse paraphenylquinone writhing, hot-plate, and tail-flick assays, in which it appeared to be acting as a μ-opioid receptor agonist. It generalized to the heroin discriminative stimulus and served as a positive reinforcer in rats and completely suppressed withdrawal signs in morphinedependent rhesus monkeys. These results suggest that the analgesic and abuse liability effects of oxycodone are likely mediated through μ-opioid receptors and provide the first laboratory report of its discriminative stimulus, reinforcing, and morphine cross-dependency effects. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Backscattered electron imaging of the undersurface of resin-embedded cells by field-emission scanning electron microscopy

In this study backscattered electron (BSE) imaging was used to display cellular structures stained with heavy metals within an unstained resin by atomic number contrast in successively deeper layers. Balb/c 3T3 fibroblasts were cultured on either 13-mm discs of plastic Thermanox, commercially pure titanium or steel. The cells were fixed, stained and embedded in resin and the disc removed. The resin block containing the cells was sputter coated and examined in a field-emission scanning electron microscope. The technique allowed for the direct visualization of the cell undersurface and immediately overlying areas of cytoplasm through the surrounding embedding resin, with good resolution and contrast to a significant depth of about 2 μm, without the requirement for cutting sections. The fixation protocol was optimized in order to increase heavy metal staining for maximal backscattered electron production. The operation of the microscope was optimized to maximize the number of backscattered electrons produced and to minimize the spot size. BSE images were collected over a wide range of accelerating voltages (keV), from low values to high values to give ‘sections' of information from increasing depths within the sample. At 3–4 keV only structures a very short distance into the material were observed, essentially the areas of cell attachment to the removed substrate. At higher accelerating voltages information on cell morphology, including in particular stress fibres and cell nuclei, where heavy metals were intensely bound became more evident. The technique allowed stepwise ‘sectional’ information to be acquired. The technique should be useful for studies on cell morphology, cycle and adhesion with greater resolution than can be obtained with any light-microscope-based system.     

Vertical sections of cultivated anchorage-dependent cells for electron microscopy

A method is described for electron microscopic preparation of cultivated cells for vertical sections using the test chamber system TCSC-1. The cells are cultivated on a special foil. They can be fixed and embedded directly in the chamber. After polymerization of the resin, the foil can be easily taken off and a second resin layer is poured upon the embedded cells. Then distinct cells can be marked under an inverted light microscope. The bilayer of the resin allows optimal conditions for vertical sections of anchorage-dependent cells.