Red AIE Luminogens with Tunable Organelle Specific Anchoring for Live Cell Dynamic Super Resolution Imaging

Lysosomes and mitochondria play an important role in maintaining cell homeostasis. Visualizing the long-term activities of lysosomes and mitochondria on the nanometer scale in live cells is essential for further understanding their functions but remains challenging due to the limitations of existing fluorescent probes, such as aggregation-caused quenching (ACQ) effect, limited signal-to-noise ratio from fluorescence “always on” in the process of targeting organelle and poor photobleaching resistance. Herein, two efficient red-emitting aggregation-induced emission (AIE) luminogens are reported, which showed “off-on” fluorescence characteristic and specific lysosomes as well as mitochondria targeting capability. Owing to their AIE characteristics, a Stokes’ shift larger than 100 nm, good biocompatibility, and excellent photostability, the AIE luminogens have been successfully utilized for high fidelity imaging of lysosomes and mitochondria. By virtue of these two probes, stimulated emission depletion (STED) images of dynamic lysosomal fusion and mitochondrial fission with a high resolution of 65.6 nm are obtained. Furthermore, the interactions between lysosomes and mitochondria in the process of mitophagy are recorded. This study also provides practical guidance for designing specific organelle targeting probes to support live cell dynamic super-resolution imaging.     

基于PLC控制的自动化生产线的产能自调节系统研究

自动生产线上输送带的速度与生产计划以及系统废品率之间无法互相调节,导致了生产效率低、废品率高等现象.为了提高自动分拣生产线的产能,本文设计了产能自调节系统;叙述了构建系统的研究思路;搭建了系统的模型;分析了系统控制方案的工作原理以及影响产能的主要因素;确定了系统的主要技术指标;构建了特定的控制算法模型;运用PLC、步进...     

Synergistic Dielectric–Magnetic Enhancement via Phase-Evolution Engineering and Dynamic Magnetic Resonance

Dielectric polarization and magnetic resonance associated with intrinsic constituent and extrinsic structure are two kinds of fundamental attenuation mechanisms for microwave absorbers, but remain extremely challenging in revealing the composition-morphology-performance correlation. Herein, hierarchical MXene/metal-organic framework derivatives with coherent boundaries and magnetic units below critical grain size are constructed to realize synergistic dielectric–magnetic enhancement by phase-evolution engineering and dynamic magnetic resonance. Specifically, phase-evolution induced inseparable interfaces, diverse incompatible phases, and defects/vacancies contribute to dielectric polarization, while closely distributed magnetic units simultaneously realize nanoscale multi-domain coupling and long-range magnetic interaction. As results, the hierarchical derivatives promise an exceptional reflection loss of −59.5 dB and an effective absorption bandwidth of 6.1 GHz. Both experimental results and theoretical calculations indicate that phase-evolution engineering and dynamic magnetic resonance maximize the absorption capability and demonstrate a versatile methodology for manipulating microwave attenuation. More importantly, the proposed multi-domain coupling and long-range magnetic interaction theories innovatively offer dynamic magnetic resonance mechanism for magnetic loss within critical grain size.     

Environmentally Adaptive Polymer Hydrogels: Maintaining Wet-Soft Features in Extreme Conditions

Hydrogels have been widely explored to adapt to different application circumstances. As typical wet-soft materials, the high-water content of hydrogels is beneficial to their wide biomedical applications. Moreover, hydrogels have been displaying considerable application potential in some high-tech areas, like brain-computer interface, intelligent actuator, flexible sensor, etc. However, traditional hydrogel is susceptive to freezing below zero, dehydration, performance swelling-induced deformation, and suffers from mechanical damage in extremely mechanical environments, which result in the loss of wet-soft peculiarities (e.g., flexibility, structure integrity, transparency), greatly limiting their applications. Therefore, reducing the freezing point, improving the dehydration/solution resistance, and designing mechanical adaptability are effective strategies to endow hydrogels with the extreme environmental adaptability, thus broadening their application fields. This review systematically summarizes research advances of environmentally adaptive hydrogels (EAHs), comprising anti-freezing, dehydration-resistant, acid/base/swelling deformation-resistant, and mechanical environment adaptive hydrogels (MEAHs). Firstly, fabrication methods are presented, including the deep eutectic solvent/ionic liquid substituent, the addition of salts, organogel, polymer network modification, and double network (DN) complex/nanocomposite strategy, etc. Meanwhile, the features of different approaches are overviewed. The mechanisms, properties, and applications (e.g., intelligent actuator, wound dressing, flexible sensor) of EAHs are demonstrated. Finally, the issues and future perspectives for EAHs’ researches are demonstrated.     

基于视觉特性的真实影像再现技术进展及展望

视觉特性研究的成果,融入到真实影像再现技术,可提供更适于人类观察的高品质图像,有利于机器视觉的研究。首先简单总结当前主要的视觉特性研究成果,包括视觉空间对抗、光谱对抗、视觉适应、视觉皮层初级处理等。综述了基于视觉特性的真实影像再现技术的研究动态和新进展,并根据算法的侧重点不同,分析了以保持颜色恒定性为主要目标的Retinex系列算法、各种动态范围压缩算法等。其中也涉及到已开展的部分工作,从原理分析、实验效果对比等角度,对研究成果与国外优秀算法做出评价。介绍了国外在软/硬件平台实现上的最新研究成果。简述进一步开展的工作及研究方向,并就其发展方向做出展望。     

无线信元反向复用及动态容量调整技术研究

根据ATM无线链路组网需求,提出了适合无线链路反向复用的编码及动态容量调整技术。该技术适用于利用微波、散射和卫星等传输手段进行ATM/MPLS无线机动组网,编码纠错门限为2×10-3,当通信节点无线传输距离增大或信道传输质量变恶劣时,通过动态容量调整技术自动降低信号速率以提高业务传输质量,该项技术特别适合新一代宽带网络。     

网格计算中基于OGSI的GT3动态适配模型研究

为简化网格应用开发者在网格计算底层平台上繁琐的编译部署发布过程,自行开发了网格计算应用快速开发工具GBuilder。开发GBuilder的目的之一是使借助其开发的网格应用能够自主适应各种网格计算底层支撑平台。就GBuilder中GT3(Globustoolkit3.0)的动态适配模型进行了探讨。提出了GT3动态适配的目标,介绍了OGSI规范中提出的网格计算服务端的组件模型,给出了根据标准接口所定义的基本规则,叙述了所提出的GT3动态适配模型各功能部件和应用流程。     

基于簇的寄存器堆功耗管理方法

本文采用软硬件协同设计技术,提出以寄存器簇为粒度对嵌入式处理器寄存器堆进行功耗管理的方法.在软件方面,面向寄存器簇的编译优化使循环程序段中寄存器的编号尽可能相邻;在硬件方面,采用寄存器簇缓冲器过滤对寄存器堆的访问并降低其动态功耗,采用基于寄存器簇的动态电压调节电路和门控预充电路降低存储单元和位线的泄漏功耗.实验结果表明,本文方法将寄存器堆的总功耗降低约44.7%,比传统方法达到了功耗、面积和延迟的更优折衷.     

采用开环模式实现深空动态模拟源设计

由于深空探测的作用距离十分遥远,最大测控距离能达到几百万公里,信号的传播时延大,因而给深空动态模拟源的设计带来了一些技术难点.针对这些难点,在结合运动方程的基础上提出了采用开环模式实现动态模拟源的设计方案.通过分析可知,该方案具有电路简单、节约成本的特点,易于在工程中实现.     

中频数字载波跟踪环路分析与计算

文中针对二阶模拟、数字锁相环模型,推导了开环和闭环传递函数。对载波跟踪环鉴相器、清除滤波器和DDS增益等做了较为详尽的理论分析,得到了环路增益和带宽的数学推导结果。系统测试结果表明,此跟踪环路计算方法经测试证明是正确的、可靠的,分机满足实际载波跟踪和数字解调的动态特性要求。