The polymerase chain reaction: Applications for the detection of foodborne pathogens

Faster methods for the detection of foodborne microbial pathogens are needed. The polymerase chain reaction (PCR) can amplify specific segments of DNA and is used to detect and identify bacterial genes responsible for causing diseases in humans. The major features and requirements for the PCR are described along with a number of important variations. A considerable number of PCR‐based assays have been developed, but they have been applied most often to clinical and environmental samples and more rarely for the detection of foodborne microorganisms. Much of the difficulty in implementing PCR for the analysis of food samples lies in the problems encountered during the preparation of template DNAs from food matrices; a variety of approaches and considerations are examined. PCR methods developed for the detection and identification of particular bacteria, viruses, and parasites found in foods are described and discussed, and the major features of these reactions are summarized.     

平行缝焊工艺的热效应研究

平行缝焊作为一种高可靠性的气密性密封方法,在陶瓷封装行业中有着愈发重要的应用。封焊过程中瞬间产生的高能量在完成密封的同时,会对陶瓷外壳造成很高的热应力残留,可能会对器件可靠性产生影响。介绍了平行缝焊的工作原理及工艺参数,并通过使用ANSYS软件对工艺过程中的各参数进行热仿真分析,研究平行缝焊工艺参数对陶瓷封装的热影响。     

铜锡合金纳米粒子的制备和性能研究

通过化学还原方法制备了铜锡合金纳米粒子,并研究分析了其的尺寸和热学性能。铜锡纳米粒子的X射线衍射分析结果显示,合成产物主要是锡纳米颗粒和铜锡合金(Cu6Sn5)纳米颗粒组成,且这些纳米粒子并未被氧化。其示差扫描量热法测得结果表明,本次合成的纳米颗粒的熔点为202.98 ℃,适合现代电子封装技术对低熔点封装材料的要求。     

水产食品熟食品和水产食品气调包装的研究

本文介绍水产食品、烘烤食品和熟食品的气调包装工艺。从混合气体配气比例、塑料包装材料的气体阻隔性、贮藏温度和包装前食品的卫生指标四个方面因素分析食品气调包装的保鲜效果。通过试验取得一些气调包装食品的保质期。     

社交媒体中转基因食品的媒介框架研究——基于美国YouTube视频网站的案例分析

社交媒体在科学传播中扮演的角色日益重要,在促进争议科学题材的传播中发挥的作用尤为显著。本文以世界上最大的社交视频网站You Tube中的转基因食品短片为案例,从传播主体、媒介框架和传播技巧三方面来分析社交媒体的框架建构过程。研究发现,在You Tube网站上美国的各种社会组织抢占了绝对的话语主导权,媒体也有积极参与,而政府和科研机构基本处于失语状态。在媒介框架上,最多的一类是关于食品安全、经济和科技的话题,且大部分都具有负面属性。在塑造技巧上,最受欢迎的短片大部分具有刺激性标题、诉诸于情感和特定价值、制造矛盾吸引受众三个显著特点。     

半导体激光器封装中热应力和变形的分析

为了解决半导体激光器封装的热应力和变形问题,利用有限元软件ANSYS对SnPb、In、AuSn三种焊料焊接激光器管芯的情况分别进行了模拟,得到了相应的热应力大小和变形情况,分析了焊料和热沉对激光器热应力和变形的影响.对比了这几种不同封装方法的激光器发光区图像的弯曲程度,验证了模拟结果.由模拟和实验结果可见,采用In焊料是减小激光器热应力和变形的最佳选择.另外,适当增加热沉厚度,选择热匹配的材料,焊接时进行预热,可减小激光器的热应力和变形.通过模拟和实验分析,提出了减小热应力和变形的方法,为优化激光器的封装设计提供了参考依据.     

基于近似模型的电子封装散热结构优化设计

针对封装散热结构优化问题中存在的难点,提出了一种基于近似模型和随机模拟的快速全局优化方法.利用Kriging方法建立封装散热结构的近似模型,重构原始的优化问题,采用随机模拟对重构出的目标函数进行寻优,从而得到最优解.采用CVT试验设计,使Kriging模型的泛化预测能力达到最大程度的发挥;在随机模拟中采用Quasi-Monte Carlo法,有效地提高了寻优的效率.以方形扁平封装器件为例,应用该方法实现了封装散热结构的优化,结果表明所提出的方法有效地解决了设计变量中含有离散变量的问题,并且大大提高了随机模拟优化的计算效率.     

基于高频探测信号的金属探测器的研究

运用于食品领域,是金属探测器的一种新用途。与其他领域相比,在食品工业所使用的金属探测器需要有较高的灵敏度。文中从提高探测信号频率的角度,探讨了频率变化对于金属探测器探测灵敏度的影响,论证了采用高频探测信号是提高金属探测器性能的有效途径。     

Bioinspired Supramolecular Slippery Organogels for Controlling Pathogen Spread by Respiratory Droplets

Surface-deposited pathogens are sources for the spread of infectious diseases. Protecting public facilities with a replaceable or recyclable antifouling coating is a promising approach to control pathogen transmission. However, most antifouling coatings are less effective in preventing pathogen-contained respiratory droplets because these tiny droplets are difficult to repel, and the deposited pathogens can remain viable from hours to days. Inspired by mucus, an antimicrobial supramolecular organogel for the control of microdroplet-mediated pathogen spread is developed. The developed organogel coating harvests a couple of unique features including localized molecular control-release, readily damage healing, and persistent fouling-release properties, which are preferential for antifouling coating. Microdroplets deposited on the organogel surfaces will be spontaneously wrapped with a thin liquid layer, and will therefore be disinfected rapidly due to a mechanism of spatially enhanced release of bactericidal molecules. Furthermore, the persistent fouling-release and damage-healing properties will significantly extend the life-span of the coating, making it promising for diverse applications.