Use of a YAP1 overexpression cassette conferring specific resistance to cerulenin and cycloheximide as an efficient selectable marker in the yeast Saccharomyces cerevisiae.

Drug-resistance markers for yeast transformation are useful because they can be applied to strains without auxotrophic mutations. However, they are susceptible to technical difficulties, namely lower transformation efficiency and the appearance of drug-resistant mutants without the marker. To avoid these problems, we have constructed a phosphoglycerate kinase (PGK) promoter-driven YAP1 expression cassette, called PGKp-YAP1. Yeast cells containing PGKp-YAP1 were resistant to cycloheximide, a protein synthesis inhibitor, and also to cerulenin, a fatty acid synthesis inhibitor, but not to other drugs tested. The transformation efficiency of PGKp-YAP1 using cerulenin selection was comparable to that using a URA3 auxotrophic marker when low concentrations of cerulenin were used. Non-transformed drug-resistant colonies did appear on the low-concentration cerulenin plates. However, these non-transformed colonies could easily be identified, based on their cycloheximide sensitivity and/or their resistance to aureobasidin A to which the transformants were sensitive. Therefore, the dual drug resistance of PGKp-YAP1 could be used as an effective selection for PGKp-YAP1 recipient cells. The PGKp-YAP1 marker was used to disrupt the LYS2 gene and to transform an industrial yeast strain, indicating that this marker can be used for efficient and reliable gene manipulations in any Saccharomyces cerevisiae strain.     

Computing distance transformations in convex and non-convex domains

It is shown that the well known two-pass sequential local transformation algorithm for computing a distance transformation in rectangular domains may fail in some convex integer domains, but that a four-pass algorithm is sufficient in all two-dimensional convex domains. For non-convex domains the number of passes necessary is shown to be generally greater. Two propagation algorithms for computing the distance transformation are described and shown theoretically and experimentally to be computationally more efficient than the sequential local transformation algorithm in non-convex domains of complex shape. The relationship of the distance transformation in non-convex domains to some more general transformations is explored.     

复合功能原理方案特征建模及其求解过程研究

研制了驱动、传动和分离等典型功能原理方案解的知识库，建立了复合功能产品原理解的特征模型，提出了复合功能原理求解的通用进`生平注解和接口匹配并行的策略，进而提出原理解接口特征的嵌套结构及其匹配进程，开发了一个复合功能产品原理方案求解的计算机支持平台，进行了实例验证。     

离心式空压机轴承技术改造

针对离心式空压机各级轴振动值超标的现象进行技术攻关,解决了制约装置安全平稳生产的瓶颈问题。     

Necessary optimality conditions in a nonsmooth control problem with variable structure

An optimal control problem with nonsmooth performance criterion described by a system of ordinary differential equations is considered. Necessary first-order optimality conditions are obtained.     

Extracting Electron Densities in N-Type GaAs From Raman Spectra: Theory

In this paper, we present the theory for calculating Raman line shapes as functions of the Fermi energy and finite temperatures in zinc blende, n-type GaAs for donor densities between 10¹⁶ cm⁻³ and 10¹⁹ cm⁻³. Compared to other theories, this theory is unique in two respects: 1) the many-body effects are treated self-consistently and 2) the theory is valid at room temperature for arbitrary values of the ratio R = (Q²/α), where Q is the magnitude of the normalized wave vector and α is the normalized frequency used in the Raman measurements. These calculations solve the charge neutrality equation self-consistently for a two-band model of GaAs at 300 K that includes the effects of high carrier concentrations and dopant densities on the perturbed densities of states used to calculate the Fermi energy as a function of temperature. The results are then applied to obtain the carrier concentrations from Fermi energies in the context of line shapes in Raman spectra due to the coupling between longitudinal optical phonons and plasmons. Raman measurements have been proposed as a non-destructive method for wafer acceptance tests of carrier density in semiconductor epilayers. The interpretation of Raman spectra to determine the majority electron density in n-type semiconductors requires an interdisciplinary effort involving experiments, theory, and computer-based simulations and visualizations of the theoretical calculations.     

强化技改决策促进技术进步和企业发展

以有效的技改投资决策理念指导企业技改投资，加快了企业技术进步，促进了企业跨越式发展，使三钢的产能从1996年年产钢50万t钢规模发展到目前的200万t钢水平，且优化了产业结构，技术经济指标从全国同行业的中下游上升到领先水平，增强了企业实力。但是，在取得显著成绩的同时，周边民营钢铁企业异军突起，面对严峻的挑战，三钢力争两年内依靠技改使产能达到300万t钢规模，并筹谋更远大的发展。     

Survivability of systems under multiple factor impact

The paper considers vulnerable multi-state series-parallel systems operating under influence of external impacts. Both the external impacts and internal failures affect system survivability, which is determined as the probability of meeting a given demand. The external impacts are characterized by several destructive factors affecting the system or its parts simultaneously.In order to increase the system's survivability a multilevel protection against the destructive factors can be applied to its subsystems. In such systems, the protected subsystems can be destroyed only if all of the levels of their protection are destroyed.The paper presents an algorithm for evaluating the survivability of series-parallel systems with arbitrary configuration of multilevel protection against multiple destructive factor impacts. The algorithm is based on a composition of Boolean and the Universal Generating Function techniques.Illustrative examples are presented.     

Analysis of Resolution of Bistatic SAR

In this paper, the spatial resolutions at different directions of bistatic synthetic aperture radar （BiSAR） have been derived from the ambiguity function. Compared with monostatic signal to noise ratio, BiSAR＇s resolutions of a fixed point target are varying with slow time since BiSAR system is space-variant. Constraints for the assumption of space-invariant bistatic topology are proposed in the paper. Moreover, under the assumption of invariance, the change of resolutions at different point in the image scene is taken into account, and we have specified two key parameters that affect resolutions directly and analyzed the way how they influence on the resolutions.     

Hydriding and Dehydriding Characteristics of Mechanically Alloyed LaMg_(17) Ni Composite Material

Magnesiumandmagnesiumalloyshavebeenin vestigatedashydrogenstoragematerialsforseveralde cadesbecausefarmorehydrogenbyweightcanbestoredinthemthaninmostoftheothercurrentlyknownhydrogenstoragealloys .Moreover ,thehighnaturalabundanceofMg ,itslightmassandenviron mentalcompatibilitypotentiallymakemagnesiumoneofthemostprospectivecandidatesforfuturehydrogenstoragematerials .Unfortunately ,thepracticalappli cationofMganditsalloyshasbeenlimitedonlytocertainstoragedevicebecauseoftheirpoorhydriding dehydr…