Label-free electrochemical immunosensor for sensitive detection of kanamycin

A novel label-free electrochemical immunosensor for sensitive detection of kanamycin based on water-soluble graphene sheet (WGS)/prussian blue-chitosan (PB-CTS)/nanoporous gold (NPG) composited film has been reported. PB was selected as an electron transfer mediator, and was modified onto the electrode together with WGS through electrostatic adsorption. Then NPG was immobilized onto the as-prepared film for biomolecules anchoring. The electroactivity of PB was greatly enhanced in the presence of WGS and NPG. It could mainly be ascribed to the fact that the good conductivity of WGS and NPG promoted electron transfer and enhanced the sensitivity. kanamycin antibody, as a model, was immobilized onto the composite film for the detection of kanamycin. Under optimum conditions, the amperometric signal of PB decreased linearly with kanamycin concentration (0.02-14 ng mL⁻¹), a linear calibration plot (y = 1.3817 + 4.7544x, r = 0.9993), resulting in a low limit of detection (6.31 pg mL⁻¹). The novel immunosensor for the detection of kanamycin in real sample with satisfactory results has been proved. In addition, this method would be easily adapted for the detection of other residual antibiotics in animal derived foods.     

Palladium nanoclusters-coated polyfuran as a novel sensor for catecholamine neurotransmitters and paracetamol

A promising electrochemical biosensor was developed by electrodeposition of palladium nanoclusters on polyfuran film modified platinum electrode. This biosensor electrode was used to determine some catecholamines, namely dopamine, epinephrine and norepinephrine, ascorbic acid and paracetamol. The method of formation of the polymer film and deposition of Pd particles plays a key role in the electroactivity of the resulting hybrid material. This sensor effectively resolved the overlapping anodic peaks of ascorbic acid (AA), dopamine (DA) and paracetamol (ACOP) into three well-defined voltammetric peaks in differential pulse voltammetry analysis. The detection limit of DA in the absence and presence of AA and ACOP are eventually the same which indicates that the oxidation processes of DA, AA and ACOP are independent and that the simultaneous measurements of the three analytes are possible without interference. The electrodeposition of Pd on polyfuran improved exceptionally the detection limit about four decades. Moreover, diffusion coefficient measurements confirmed the fast electron transfer kinetics of the electrochemical oxidation of the analyte molecules at the sensor/solution interface. It is very interesting to note that the electrocatalytic effect of PF/Pd composite has been increased to be sometimes 21 times that of the pristine PF which has been considered for a long time to be of low conductivity and attracted low attention as a result of the difficulty of its formation and poor conductivity.     

广西镇龙山金（银）矿的构造控制特征

本文阐述了广西镇龙山金（银）矿化集中区内主要断裂构造的基本特征,并指出,其中规模较大的,与矿化关系较密切的主要是近ＥＷ向,次为近ＳＮ向断裂,它们组合成线状,交叉状和放射状断裂带,并严格控制着区内岩浆岩、矿化带、矿脉、矿体和“富矿包“的空间展布。这对找矿评价和预测盲矿体都具有实际意义。     

用流体静力学称量法测定黄金饰品中金的含量

本文研究了用流体静力学称量法，通过测定密度计算金饰品中金的含量，该法简单实用，设备投产少，能满足用户日常检定的需要。该法克服了“试金石”法检定黄金的粗糙性，又解决了电子探针在测试过程中，可能对材料成人偏析所造成的误差。     

勘查砂金的最新技术KJ法

本文介绍了朝鲜民主主义人民共和国金策工业大学金致崇教授发明的根据磁场调查勘查砂金的最新技术(简称KJ法)。这项技术是不用钻探和井探,而只用仪器探出砂金的矿体形状、大小、埋深及品位。     

基于光源调制技术的金标试纸条定量分析仪器

基于光电检测原理,研发了一套可用于金标免疫试纸条定量检测的分析仪器。采用绿光发光二极管(LED)作为发射光源,以光电二极管接收试纸条反射光,并通过步进电机带动检测系统扫描试纸条待检区域。系统采用光源信号的调制与接收信号的解调技术,以消除环境干扰噪声。实验结果表明,该仪器检测结果准确,与真实值之间误差<1%;稳定性高,变异系数CV<2%。     

高原寒冷区地球电化学方法寻找隐伏金矿对比试验

为了解高原寒冷景观区隐伏金矿地电化学异常特征,选取辛曲矿区和扎家同哪矿区进行对比试验。结果表明,两个矿区的地电提取Au含量均较低,且多为难以连片的狭窄异常,但矿体的分布基本限定在地电化学综合异常范围内。两个矿区有不同的异常特征：产出较陡的辛曲金矿异常多呈狭窄的单峰,峰值与矿体头部相对应;而产出较缓的扎家同哪金矿,异常呈多峰异常形态,在矿头对应部位有异常峰,且异常沿矿体倾斜方向呈阶梯式变弱,表明在高原寒冷景观区地球电化学方法对隐伏金矿体赋存范围和产出形态均有指示作用。结合地电化学异常成因机制、成矿地质条件,初步构建了高原寒冷景观条件下破碎带型金矿地电化学找矿模型。     

有机质的地质热示踪意义及其成矿作用——以桂西北卡林型金矿为例

在卡林型金矿中有机质常与矿石共生,根据有机地球化学理论,可利用有机质示踪地质热事件。以桂西北卡林型金矿为例,研究有机质的基本类型及地球化学特征,探讨了桂西北地区卡林型金矿中有机质对地质热事件的示踪及其与金矿成矿的关系：①桂西北地区各矿区的有机质可划分为原生型干酪根和迁移型有机流体两种基本类型,其中,迁移型有机质主要为凝胶化有机质,主要是地层原生有机质在地质热事件的影响下由于热降解作用生成的有机流体的残余物,其形成、演化与成矿关系极为密切;②桂西北地区卡林型矿区地层中的有机质成熟作用由矿体向近矿围岩、再过渡到矿体外围,其氯仿沥青A含量及其A/C值由大逐渐变小,反映成矿过程中,成矿的地质热事件对于地层原生有机质影响所产生的热效应,从矿体到外围地层逐渐减弱;③地质热事件对于金矿区赋矿地层中有机质产生了明显的改造。     

广西金牙金矿毒砂 Re-O s同位素测年和硫同位素示踪

金牙金矿的载金矿物主要为毒砂,次为含砷黄铁矿。毒砂矿物结构均一,为主成矿阶段热液成因,相较于成因复杂的环带状含砷黄铁矿更适合于同位素测年和示踪。通过对该矿床毒砂的单矿物Re-Os同位素测年和S同位素分析,获得Re-Os等时线年龄为（206±22）Ma,反映金矿形成于晚三叠世末期,大致相当于印支期挤压造山向燕山期拉伸的构造转换期。毒砂δ34 S值为-9‰～-3.9‰,平均值为-5.3‰,具有沉积硫的特征,推断硫来自地层,成矿流体为盆地建造水。由此认为金牙金矿是一个与盆地流体和沉积物有关的后生热液矿床。