对建设中国先进文化的几点认识

充分认识文化和先进文化的本质特征,领会“三个代表”重要思想中关于“代表中国先进文化前进方向”的深刻内涵,研讨我国先进文化建设面临的新课题,努力实践“三个代表”,积极发展先进文化。     

Nisin expression production from Lactococcus lactis in milk whey medium

BACKGROUND: Nisin is a commercially available bacteriocin produced by Lactococcus lactis ATCC 11454 and used as a natural agent in the biopreservation of food. In the current investigation, milk whey, a byproduct from dairy industries was used as a fermentation substrate for the production of nisin. Lactococcus lactis ATCC 11454 was developed in a rotary shaker (30 °C/36 h/100 rpm) using two different media with milk whey (i) without filtration, pH 6.8, adjusted with NaOH 2 mol L^?1 and without pH adjustment, both autoclaved at 121 °C for 30 min, and (ii) filtrated (1.20 µm and 0.22 µm membrane filter). These cultures were transferred five times using 5 mL aliquots of broth culture for every new volume of the respective media. RESULTS: The results showed that culture media composed of milk whey without filtration supplied L. lactis its adaptation needs better than filtrated milk whey. Nisin titers, in milk whey without filtration (pH adjusted), was 11120.13 mg L^?1 in the second transfer, and up to 1628‐fold higher than the filtrated milk whey, 6.83 mg.L^?1 obtained in the first^t transfer. CONCLUSIONS: Biological processing of milk byproducts (milk whey) can be considered a profitable alternative, generating high‐value bioproducts and contributing to decreasing river disposals by dairy industries. Copyright © 2008 Society of Chemical Industry     

Effect of temperature on recombinant protein production using the Bm5/Bm5.NPV expression system

A series of experiments have been conducted using a recombinant baculovirus/insect cell expression system (Bm5/Bm5.NPV.CAT) to establish the optimum temperature for both cell growth and virus infection. Bm5 cell growth was found to be limited at temperatures below 22°C and ceased completely at temperatures above 34°C. In the range between 24 and 28°C, final cell densities always reached 96% of the highest achievable viable cell density. The shortest population doubling time was obtained at 28°C. Overall, a consistent increase in metabolism with increasing temperatures was observed. During the infection/viral replication phase, an increase in the temperature from 25 to 31°C resulted in a faster decrease in viable cell density and an earlier production of chloramphenicol acetyltransferase (CAT). Furthermore, protein yield at temperatures above 28°C was significantly reduced. Overall, the best temperature for the infection phase for the Bm5/Bm5.NPV expression system was found to be 25°C when the cells are cultured in serum free media.     

“中国式”非显形奢华

奢侈品在中国逐渐成为热门产业,建立在精神层面上的“非显形奢华”也渐为众人关注。中国拥有深厚的文化底蕴,足以创造撼动视觉新体验的新“奢华”美学。当创意市场不断推进,变得成熟之后,中国的“非显形奢华”定会有更高的价值,奢华不再是炫耀身份的代名词,而是融合民族特色、具有独特的设计品位,真正意义上的顶级产品或服务,是一种低调、内敛、优雅的“非显形奢华”。     

全球化进程中建筑的趋同

对全球化进程中建筑趋同的原因进行了分析,探讨了全球化对中国建筑的影响,并对我国当代建筑创作问题提出了见解,以使人们对建筑全球化的趋同有一个较为清晰的认识。     

明清图书翻译的特征、种类及作用

本文对我国在明末和清代西学东渐过程中的图书翻译进行了梳理和剖析,总结了这一时期西学翻译的历史概况,阐述了东西文化交流中翻译图书的重要作用,以及历史上有识之士对于西学翻译的贡献。     

晋煤集团赵庄煤矿建筑外部空间规划浅谈

通过对晋煤集团赵庄煤矿建筑外部空间规划的实例分析,综合考虑工业、行政、生活区的不同功能,构建园林、人文、旅游式矿山,从文化提炼、总体把握、景观构成等方面解决了煤矿建设中的可持续发展问题,以达到良好的规划效果。     

中华丹参酒的研制

以丹参为主 ,配以12种中药材 ,经热回流提取 ,回流时夹层气压为0.2MPa,锅内气压小于0.05MPa,时间2h,按配比将提取液与酒母液在冷冻罐内混合 ,在0.05MPa下反吹混匀 ,取上清液为半成品 ,再经过滤等处理为成品。(孙悟)     

水文化研究的现代视野

水文化与人类文明同步。与水文化作为母体文化的传统优势相比,现代水文化研究的冲击力显得极其不足。应该在现代文化的视野下寻找水文化研究更为宽阔的出路。现代水利科技是水文化的主体,水资源短缺的危机为现代水文化提出了挑战;水与经济互动发展拓展了现代水文化的空间;农村饮水安全问题使水文化与人文素质相互渗透;农村水库生活与和谐社会建设为水文化提供了新的诠释。     

Neural networks modeling signal responses and taxol production of cultured Taxus chinensis cells induced by bio-elicitor

Quantitatively describing the signal transduction process is important for understanding the mechanism of signal regulation in cells, and thus, poses both a challenge and an opportunity for chemical and biochemical engineers. An artificial neural network (ANN), in which we took the signal molecules as neural nodes, was constructed to simulate the generation of active oxygen species (AOS) in Taxus chinensis cells induced by a bio-elicitor. The relative contents of AOS in cells predicted by the ANN model agreed well with the experimental data and three notable stages of AOS increase were observed from the 3D figure of AOS generation. The robustness of AOS trajectories indicated that signal regulation in vivo was an integral feedback control model that ensured the adaptation of Taxus chinensis to environmental stress. The artificial neural network was able to predict taxol production as well as determine the optimal concentration of oligosaccharides needed for it.