The GNAQ T96S Mutation Affects Cell Signaling and Enhances the Oncogenic Properties of Hepatocellular Carcinoma

Hepatocellular carcinoma (HCC), the most common malignant tumor in the liver, grows and metastasizes rapidly. Despite advances in treatment modalities, the five-year survival rate of HCC remains less than 30%. We sought genetic mutations that may affect the oncogenic properties of HCC, using The Cancer Genome Atlas (TCGA) data analysis. We found that the GNAQ T96S mutation (threonine 96 to serine alteration of the Gαq protein) was present in 12 out of 373 HCC patients (3.2%). To examine the effect of the GNAQ T96S mutation on HCC, we transfected the SK-Hep-1 cell line with the wild-type or the mutant GNAQ T96S expression vector. Transfection with the wild-type GNAQ expression vector enhanced anchorage-independent growth, migration, and the MAPK pathways in the SK-Hep-1 cells compared to control vector transfection. Moreover, cell proliferation, anchorage-independent growth, migration, and the MAPK pathways were further enhanced in the SK-Hep-1 cells transfected with the GNAQ T96S expression vector compared to the wild-type GNAQ-transfected cells. In silico structural analysis shows that the substitution of the GNAQ amino acid threonine 96 with a serine may destabilize the interaction between the regulator of G protein signaling (RGS) protein and GNAQ. This may reduce the inhibitory effect of RGS on GNAQ signaling, enhancing the GNAQ signaling pathway. Single nucleotide polymorphism (SNP) genotyping analysis for Korean HCC patients shows that the GNAQ T96S mutation was found in only one of the 456 patients (0.22%). Our data suggest that the GNAQ T96S hotspot mutation may play an oncogenic role in HCC by potentiating the GNAQ signal transduction pathway.     

螺旋齿轮塑件注塑模设计

分析了斜齿轮塑件的工艺特性,给出了实用的注塑模结构,介绍了斜齿轮注塑模各系统的结构设计,论述了模具的工作原理。该模具利用定模推出机构和推管旋转脱模机构解决了螺旋齿轮脱模难的问题。模具结构紧凑,推出可靠,安装维修操作方便,成型塑件质量好,生产效率高。     

基于Pro／E参数化斜齿轮建模技术

应用Pro／E软件的参数化造型功能，不需要二次开发，直接创建渐开线斜齿圆柱齿轮模型，对直齿、人字齿齿轮的建模具有借鉴作用。     

A helical drive in-pipe robot based on compound planetary gearing

The modern society is fuelled by very comprehensive grids of gas and liquid pipelines. In recent years, various in-pipe robots have been developed for inspection and maintenance tasks inside such pipes. In this paper, a novel in-pipe robot is proposed and developed for gas/oil well interventions at thousands of meters downhole. Due to the nature of such intervention, in-pipe robot design must be capable of carrying a very large payload, as large as 2500?N inside a pipe with diameter as small as 54?mm. The proposed design concept is based on a compound planetary gearing system. One of the major novelties of this design is the use of pipe wall as a ring gear for one stage of the compound planetary gear system; the other novelty is the generation of helical angle when the planetary gears are expanded to press on the pipe wall. The proposed concept is compact, efficient, and has never been reported before. In this paper, the helical angle, the velocity, and load capability of the proposed system will be analyzed. The load transportation capability of the proposed robot is also measured based on an experiment. Initial data have shown great potential in carrying large payloads.     

Gas hold-up in non-newtonian solutions in a bubble column with internals

Bubble characteristics and gas hold-up were studied in a two phase (air-aqueous CMC solution) bubble column provided with helical coils and straight tubes as internals. The effects of superficial gas velocity, rheological properties, and volume fraction covered by the internals, on gas hold-up were studied. Hold-up values determined directly and by simultaneous pressure drop measurements matched well. Enhancement of gas hold-up values up to 55 per cent was achieved in systems using internals. The gas hold-up results were also compared with the values obtained from correlations reported in the literature.     

螺旋侧板厚度对浮式风力机Spar平台涡激载荷的影响

Spar平台因其特殊的深吃水立柱式结构,在来流影响下极易发生涡激运动。为探究不同厚度螺旋侧板对浮式风力机Spar平台涡激载荷的影响,基于CFD方法,针对不同厚度螺旋侧板,对平台进行了数值模拟并详细分析平台的升阻力系数、压力场及涡量场等流场参数。结果表明:螺旋侧板可有效抑制涡激载荷,且随厚度增加,升力系数幅值先增加后减小,阻力系数逐步减小;在研究范围内,螺旋侧板厚度为0.10 D时,升力系数幅值最小,阻力系数与原型平台在同一范围内,对涡激载荷的抑制效果最好。     

经孔板形成旋转流的管壳式换热器壳程传热及流阻的研究

对螺旋折流孔板管壳式换热器壳程的传热与流体阻力做了研究 ,给出换热器壳程传热与流阻的计算关联式 ,并采用实验模型对换热器壳程流体旋转流的阻力系数与传热管的局部传热系数做了测试 ,且对光滑和菱形翅片两种管型作了对比     

Flow regimes,hold–up and pressure drop for two phase flowin helical coils

Experimental results on flow pattern, hold–up and pressure drop are presented for cocurrent upward and downward air water flow in helical coils. A tube of 0.01 m internal diameter was used and the ratio of coil to tube diameter was varied from 11 to 156.5. Water flow rate was varied from 4.9 × 10-⁶ m³/s to 92 × 10-⁶ m³/s while the range of gas flow rate covered was 83 × 10-⁶ m³/s to 610 × 10-⁶ m³/s. A new mechanistic approach is proposed to correlate pressure drop data in coils. The proposed model retains the identity of each phase and separately accounts for the effects of curvature and tube inclination resulting from the torsion of the tube. This makes it possible to use a single model to predict pressure drop for both upward and downward two–phase flow in coiled tubes. Required correlations for hold–up, interfacial friction factor and friction factors for individual phases are provided.     

基于云计算的 Excel 数据处理效率优化研究*

为了提高Excel软件的数据处理效率,需建立一个能满足高精确度、高效率、易使用的计算环境。论文建立圆柱螺线优化插值模型,在Excel中通过五组测斜数据计算井眼曲率,再根据云计算可弹性增加计算节点的特点,将Sympho‐ny DE并行软件搭建在本地私有云环境下,通过增加计算节点数目对比运算效率与计算精度。结果表明：随着计算节点数的增加,运算效率大大提高,计算结果更加精确。     

Experimental Study on the Expansion Uniformity of Armature

A measurement system has been developed based on high-precision printed probes in printed circuit board (PCB) and steep rising-time probe adapters,which can be applied to study the expansion uniformity of armature in a helical magnetic flux compression generator (HFCG).The influences of wall thickness and initiation position on the expansion uniformity of armature in HFCG were experimentally investigated.The results show that the armature with thinner wall thickness will easily rupture due to the high pressure of detonation products inside,the armature with larger wall thickness will easily crack due to the tensile stress on the outer surface of the wall,the influence of the end effect on the expansion uniformity can be ignored if the distance between the first group of probe and the initiation point is more than 3 times the armature diameter.