水下设备安装过程及其影响因素分析

为了提高水下设备安装精度与工作效率,基于海上工作经验及查阅国内外相关文献,对水下设备的安装过程进行了研究,其总体过程大致分为5个阶段,即装船固定、提升操作、穿越飞溅区、深水下放、落地坐放;分析了影响水下设备安装过程的主要因素,其中水深主要影响吊放系统和设备在水下的横向偏移;波浪载荷主要应用船舶的动态响应,在设备经历穿越飞溅区阶段时对设备及缆绳造成损伤的风险较大;水下设备自身参数主要影响安装方式和安装船舶的选用。     

Chronically Elevated Exogenous Glucose Elicits Antipodal Effects on the Proteome Signature of Differentiating Human iPSC-Derived Pancreatic Progenitors

The past decade revealed that cell identity changes, such as dedifferentiation or transdifferentiation, accompany the insulin-producing β-cell decay in most diabetes conditions. Mapping and controlling the mechanisms governing these processes is, thus, extremely valuable for managing the disease progression. Extracellular glucose is known to influence cell identity by impacting the redox balance. Here, we use global proteomics and pathway analysis to map the response of differentiating human pancreatic progenitors to chronically increased in vitro glucose levels. We show that exogenous high glucose levels impact different protein subsets in a concentration-dependent manner. In contrast, regardless of concentration, glucose elicits an antipodal effect on the proteome landscape, inducing both beneficial and detrimental changes in regard to achieving the desired islet cell fingerprint. Furthermore, we identified that only a subgroup of these effects and pathways are regulated by changes in redox balance. Our study highlights a complex effect of exogenous glucose on differentiating pancreas progenitors characterized by a distinct proteome signature.     

A Novel Volume-Stable Collagen Matrix Induces Changes in the Behavior of Primary Human Oral Fibroblasts,Periodontal Ligament,and Endothelial Cells

The aim of the present study was to investigate the influence of a novel volume-stable collagen matrix (vCM) on early wound healing events including cellular migration and adhesion, protein adsorption and release, and the dynamics of the hemostatic system. For this purpose, we utilized transwell migration and crystal violet adhesion assays, ELISAs for quantification of adsorbed and released from the matrix growth factors, and qRT-PCR for quantification of gene expression in cells grown on the matrix. Our results demonstrated that primary human oral fibroblasts, periodontal ligament, and endothelial cells exhibited increased migration toward vCM compared to control cells that migrated in the absence of the matrix. Cellular adhesive properties on vCM were significantly increased compared to controls. Growth factors TGF-β1, PDGF-BB, FGF-2, and GDF-5 were adsorbed on vCM with great efficiency and continuously delivered in the medium after an initial burst release within hours. We observed statistically significant upregulation of genes encoding the antifibrinolytic thrombomodulin, plasminogen activator inhibitor type 1, thrombospondin 1, and thromboplastin, as well as strong downregulation of genes encoding the profibrinolytic tissue plasminogen activator, urokinase-type plasminogen activator, its receptor, and the matrix metalloproteinase 14 in cells grown on vCM. As a general trend, the stimulatory effect of the vCM on the expression of antifibrinolytic genes was synergistically enhanced by TGF-β1, PDGF-BB, or FGF-2, whereas the strong inhibitory effect of the vCM on the expression of profibrinolytic genes was reversed by PDGF-BB, FGF-2, or GDF-5. Taken together, our data strongly support the effect of the novel vCM on fibrin clot stabilization and coagulation/fibrinolysis equilibrium, thus facilitating progression to the next stages of the soft tissue healing process.     

Biomarkers for Cancer Cachexia: A Mini Review

Cancer cachexia is a common condition in many cancer patients, particularly those with advanced disease. Cancer cachexia patients are generally less tolerant to chemotherapies and radiotherapies, largely limiting their treatment options. While the search for treatments of this condition are ongoing, standards for the efficacy of treatments have yet to be developed. Current diagnostic criteria for cancer cachexia are primarily based on loss of body mass and muscle function. However, these criteria are rather limiting, and in time, when weight loss is noticeable, it may be too late for treatment. Consequently, biomarkers for cancer cachexia would be valuable adjuncts to current diagnostic criteria, and for assessing potential treatments. Using high throughput methods such as “omics approaches”, a plethora of potential biomarkers have been identified. This article reviews and summarizes current studies of biomarkers for cancer cachexia.     

基于MMS替代协议的变电站安全通信技术研究及应用

目前,绝大多数变电站基于IEC61850标准建设,站内通信协议主要包括制造报文规范(MMS)、面向通用对象的变电站事件(GOOSE)、采样测量值(SMV)等,在工程应用实践中逐渐暴露出一些不足与安全隐患.为此,基于MMS替代协议,重点介绍了其实现的功能与安全防护技术.在功能方面,MMS替代协议增加了多个服务,并优化了现有MMS协议的不足;在安全防护技术方面,MMS替代协议的应用层实现了基于SM2算法与调度证书的双向身份认证,杜绝未授权设备的非法接入、攻击,从协议源头杜绝设备连接的不安全性;MMS替代协议的传输层实现了基于SM2算法与调度证书的全链路加密、认证,确保传输数据报文防伪造、防抵赖、防窃取,保护数据报文的机密性、完整性.测试结果表明MMS替代协议在工程应用中具有一定的参考意义.     

影响中小企业球铁质量稳定的因素

分析了影响中小企业球铁生产稳定性的关键因素,指出在一定的原材料条件下,目前中小型企业生产球铁存在的问题是温度偏低,孕育方法不当。     

类金刚石膜的制备及其影响因素

概述了近年来制类金刚石膜的新技术如双射频辉光放电（RF－RF）法，射频－直流辉光放电（RF－DC）法和微波－射频（MW－RF）法等，并且对影响类金刚石膜制备及性能的因素如基体材料，制备类金刚石膜时在膜中添加元素，类金刚石膜与基材之间的中间层等进行了较全面的分析。     

锡磷青铜合金水平连铸工艺参数的优化

铸造锡磷青铜合金组织中存在显微缩松、枝晶偏析和反偏析是冷加工报废的主要原因.通过连铸工艺参数的正交实验,建立了轧制成材率与主要工艺参数之间的神经网络描述,网络模型与遗传算法结合对工艺参数的组合进行了优化,改善了铸坯的组织,提高了轧制带材成材率.     

U形件弯曲影响回弹因素模拟分析

板材在弯曲过程中存在的最突出的问题是弯曲回弹难以精确控制.弯曲卸载后产生的回弹,使弯曲件的形状和尺寸与模具工作部分的形状和尺寸不符.弯曲件的最后形状与整个变形过程有关,模具几何参数、材料性能参数等都会对回弹产生很大影响.在板材弯曲成形智能化控制系统中,准确确定实时识别和预测模型的输入、输出参数是弯曲智能化控制的成功与否以及回弹控制精度高低的关键.本文采用Ls-dyna软件,对U形件弯曲影响回弹的因素进行了分析,得出了影响回弹规律的主要因素.为U形件弯曲智能化控制神经网络参数识别及预测模型输入、输出参数的选择提供依据.