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The PET18 locus of Saccharomyces cerevisiae: a complex locus containing multiple genes 总被引:13,自引:0,他引:13
The basis of pleiotropy shown by the pet18 mutants of Saccharomyces cerevisiae (rho-0,KIL-0 and temperature sensitive growth) was examined by cloning the fragment which complements the defect in growth at 37 degrees C of the pet18 mutants. The cloned DNA could complement the defect in the maintenance of the killer plasmid but did not give the cell the ability to maintain mitochondrial DNA. Sequence analysis of the cloned DNA revealed the presence of four open reading frames, at least two of which are necessary for the complementation activity. By using the cloned DNA as a probe, we found that two independent pet18 mutants have a deletion covering the entire sequence contained in the probe. From these results we predict that the traits of the pet18 mutants that concern temperature sensitivity and killer of the pet18 mutants are controlled by a separate gene(s) from that which participates in the maintenance of mitochondrial DNA. 相似文献
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Kreppner Jana M.; Rutter Michael; Beckett Celia; Castle Jenny; Colvert Emma; Groothues Christine; Hawkins Amanda; O'Connor Thomas G.; Stevens Suzanne; Sonuga-Barke Edmund J. S. 《Canadian Metallurgical Quarterly》2007,43(4):931
Longitudinal analyses on normal versus impaired functioning across 7 domains were conducted in children who had experienced profound institutional deprivation up to the age of 42 months and were adopted from Romania into U.K. families. Comparisons were made with noninstitutionalized children adopted from Romania and with nondeprived within-U.K. adoptees placed before the age of 6 months. Specifically, the validity of the assessment, the degree of continuity and change in levels of functioning from 6 to 11 years, and the factors in the pre- and postadoption environment accounting for heterogeneity in outcome were examined. Pervasive impairment was significantly raised in children experiencing institutional deprivation for ≥6 months of life, with a minority within this group showing no impairment. There was no additional significant effect of duration of deprivation beyond the 6-month cutoff, and few other predictors explained outcome. The pattern of normality/impairment was mainly established by 6 years of age, with considerable continuity at the individual level between 6 and 11 years. The findings are discussed in terms of the possibility of a sensitive period for development. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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介绍了一个基于Windows操作系统的数据采集软件,该软件用于使用重心法确定的位置信息的位置灵敏探测器,具有方便的人机交互界面,使用专用驱动程序采集数据。 相似文献
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Thi Thi Nge Makiko Yamaguchi Naruhito Hori Akio Takemura Hirokuni Ono 《应用聚合物科学杂志》2002,83(5):1025-1035
Polyelectrolyte complex based on chitosan and acrylic acid monomer by photoinitiated free‐radical polymerization in the absence of crosslinker showed a large transition in swelling in response to changes in pH of surrounding medium. Their ability to swell arises from polyelectrolyte interactions and molecular structure of the complex. The main properties of interest that related to the molecular structure, swelling volumes, glass transition temperature, and elastic modulus of the complex were investigated. The effect of water content, the only variable in the sample component, played an important role in molecular structure of the complex and as a consequence, the extent of intermolecular linkage, especially amide bonds which in turn governed the degree of swelling of the polyelectrolyte complex in this study. The decreased degree of swelling and higher temperature shift of glass transition temperature was found with increased water content, whereas increased modulus of elasticity of dry complex was found in lower water content of synthesis component. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 83: 1025–1035, 2002 相似文献
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高含酸性气碳酸盐岩气藏流体敏感性实验研究 总被引:2,自引:0,他引:2
对川东北雷口坡组、嘉陵江组、飞仙关组、长兴组高含H,S及032的碳酸盐岩储层流体敏感性进行了实验研究,结果表明岩样速敏损害弱~强,水敏和盐敏损害中偏弱~极强,碱敏损害严重。岩石学分析揭示,川东北高含酸性气碳酸盐岩储层发育伊利石、微晶石英、白云石、方解石、硫化钙等潜在损害物质。钻井完井液侵入会破坏储层各物质间的原始动态平衡,诱发储层损害。主要损害机理为:①储层流体pH增加引起黏土矿物以及微晶石英失稳;②碱液与微晶石英、长石、白云石反应生成硅酸盐、高岭石、水镁石等新矿相;③流体矿化度改变可降低伊利石微粒间的连接力;④硫化钙水解产生OH^-,过量的OH-与Ca^2+结合形成氢氧化钙沉淀;⑤储层含水饱和度和流体离子浓度改变,致使焦沥青脱附并在储层深部沉积;⑥硬石膏水化膨胀、分散运移。针对该气藏损害机理,可采用屏蔽暂堵技术以形成优质滤饼,有效降低储层流体敏感性损害。图5表2参10。 相似文献
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