Texture analysis of poly-adenylated mRNA staining following global brain ischemia and reperfusion

Texture analysis provides a means to quantify complex changes in microscope images. We previously showed that cytoplasmic poly-adenylated mRNAs form mRNA granules in post-ischemic neurons and that these granules correlated with protein synthesis inhibition and hence cell death. Here we utilized the texture analysis software MaZda to quantify mRNA granules in photomicrographs of the pyramidal cell layer of rat hippocampal region CA3 around 1 h of reperfusion after 10 min of normothermic global cerebral ischemia. At 1 h reperfusion, we observed variations in the texture of mRNA granules amongst samples that were readily quantified by texture analysis. Individual sample variation was consistent with the interpretation that animal-to-animal variations in mRNA granules reflected the time-course of mRNA granule formation. We also used texture analysis to quantify the effect of cycloheximide, given either before or after brain ischemia, on mRNA granules. If administered before ischemia, cycloheximide inhibited mRNA granule formation, but if administered after ischemia did not prevent mRNA granulation, indicating mRNA granule formation is dependent on dissociation of polysomes. We conclude that texture analysis is an effective means for quantifying the complex morphological changes induced in neurons by brain ischemia and reperfusion.     

Estimating extreme temperature differences in steel box girder using long-term measurement data

The extreme temperature differences in fiat steel box girder of a cable-stayed bridge were studied.Firstly,by using the long-term measurement data collected by the structural health monitoring system installed on the Runyang Cable-stayed Bridge,the daily variations as well as seasonal ones of measured temperature differences in the box girder cross-section area were summarized.The probability distribution models of temperature differences were further established and the extreme temperature differences were estimated with a return period of 100 years.Finally,the temperature difference models in cross-section area were proposed for bridge thermal design.The results show that horizontal temperature differences in top plate and vertical temperature differences between top plate and bottom plate are considerable.All the positive and negative temperature differences can be described by the weighted sum of two Weibull distributions.The maximum positive and negative horizontal temperature differences in top plate are 10.30 ℃ and -13.80 ℃,respectively.And the maximum positive and negative vertical temperature differences between top plate and bottom plate are 17.30 ℃ and-3.70 ℃,respectively.For bridge thermal design,there are two vertical temperature difference models between top plate and bottom plate,and six horizontal temperature difference models in top plate.     

基于三网融合的数字机顶盒的设计与应用

数字电视机顶盒是随着电视系统的更新而推出的家庭数字终端产品,其功能是把各种数字信号通过电视终端展示出来。结合实际说明了现有的数字机顶盒的设计方法,并从三网融合最新的业务需求出发,分析了新业务需求对数字机顶盒的功能要求,提出了基于三网融合的数字机顶盒的设计方案,并讨论了三网融合数字机顶盒的应用方向。     

开式箱体多孔衬套的组装及工装设计

利用工装安装制作开式箱体多孔衬套的加工方式,将铆焊技术与机械加工技术进行巧妙的结合,使制作流程更加紧凑、工序缩减、制作周期更短、加工难度降低。生产实践表明采用铆焊技术与机械加工相结合的制造方法既保证了工件的尺寸精度和质量要求,又提高了生产效率,节约了生产制造成本,效益显著。     

手掘式顶管中箱梁顶进力计算研究

通过辨析普通手掘式顶管施工中顶进力计算公式的特点,对顶进施工中的箱梁进行了受力分析,推导得到了手掘式顶管中矩形箱梁的顶进力实用计算公式,且基于某工程的实测数据,将实用计算公式计算的结果与实测数据进行了对比,结果较为合理,从而验证了实用计算公式的合理性与可靠性.     

连续刚构箱梁剪力滞效应分析

以靖远黄河大桥这一五跨连续刚构箱梁桥为工程背景,运用当量截面法计算靖远桥的剪力滞系数。分析其简化计算,将结果与ANSYS分析结果进行比较。     

针对类球形对象的改进光线投射算法

为提高光线投射算法的绘制速度和图像绘制质量,提出了一种针对类球形对象的改进光线投射算法。该算法首先设置球形包围盒的方法剔除对最后绘图结果没有影响的光线投射,用快速求交的方法来提高获取采样点的速度,通过自适应采样的方法加入新的采样点来提高绘制图像的质量。实验结果表明该算法不仅比传统方法绘制出的图像质量清晰,并且提高了算法的执行速度。     

基于Qt 的电纸书图形用户界面设计

电子书在日常生活中应用广泛,为了便于用户直观、舒适地阅读电子书,电纸书应运而生。在Linux操作系统上,利用Qt图形用户界面开发工具,实现电纸书图形用户界面的设计,并在电子纸上实现文本文档的显示。程序实现了主窗口和对话框的创建,以及主窗口和对话框之间的切换与交互。同时,在加载文本文档的过程中,实现对不同编码方式的文本文档的解码及分页。     

薄壁箱型结构位移参数的动态Bayes随机估计

应用薄壁箱型结构的有限条控制方程,建立了薄壁箱型结构位移参数的动态B ayes误差函数和相应的动态B ayes均值及方差表达式.利用迭代步长的一维自动寻优方案,结合共轭梯度法研究了薄壁箱型结构位移参数的动态B ayes估计方法.通过典型算例分析,获得薄壁箱型结构位移参数先验信息准确性判定方法及结构位移参数动态B ayes随机估计的相关结论.