Effects and Mechanism of Particulate Matter on Tendon Healing Based on Integrated Analysis of DNA Methylation and RNA Sequencing Data in a Rat Model

Exposure to particulate matter (PM) has been linked with the severity of various diseases. To date, there is no study on the relationship between PM exposure and tendon healing. Open Achilles tenotomy of 20 rats was performed. The animals were divided into two groups according to exposure to PM: a PM group and a non-PM group. After 6 weeks of PM exposure, the harvest and investigations of lungs, blood samples, and Achilles tendons were performed. Compared to the non-PM group, the white blood cell count and tumor necrosis factor-alpha expression in the PM group were significantly higher. The Achilles tendons in PM group showed significantly increased inflammatory outcomes. A TEM analysis showed reduced collagen fibrils in the PM group. A biomechanical analysis demonstrated that the load to failure value was lower in the PM group. An upregulation of the gene encoding cyclic AMP response element-binding protein (CREB) was detected in the PM group by an integrated analysis of DNA methylation and RNA sequencing data, as confirmed via a Western blot analysis showing significantly elevated levels of phosphorylated CREB. In summary, PM exposure caused a deleterious effect on tendon healing. The molecular data indicate that the action mechanism of PM may be associated with upregulated CREB signaling.     

索板法布筋方式及受力性能研究

本文探讨了无粘结预应力混凝土板的新的布筋方式,并给出理论计算方法.按此方式配筋,可节约钢材50％以上.该项技术已通过省级鉴定,并为国家经贸委资金资助项目.     

考虑P△效应的体外预应力梁的M-φ分析法

本文推导了T型截面梁的弯矩-轴力-曲率关系,提出了分析大偏心体外预应力筋的应力增量和梁弯曲性能的通用方法。在本方法中,体外筋的效应表示为作用在转向座和锚具处的等效荷载,梁(不含体外筋)的内力是由外荷载和等效荷载共同引起的。把静定梁的变形曲线简化为由单元节点位移所组成的多边形,把DP效应的附加弯矩转化为节点上的集中力,较方便地考虑了这一效应。比较荷载作用前后,转向座和锚具的变形差,计算出体外筋的应变和应力。因此这一方法考虑了体外筋的变形协调条件,同时自动地考虑了体外筋偏心距的损失。计算结果与文献[2,4]相吻合。     

Evaluation of Sodium Relaxation Times and Concentrations in the Achilles Tendon Using MRI

Sodium magnetic resonance imaging (MRI) can be used to evaluate the change in the proteoglycan content in Achilles tendons (ATs) of patients with different AT pathologies by measuring the ²³Na signal-to-noise ratio (SNR). As ²³Na SNR alone is difficult to compare between different studies, because of the high influence of hardware configurations and sequence settings on the SNR, we further set out to measure the apparent tissue sodium content (aTSC) in the AT as a better comparable parameter. Ten healthy controls and one patient with tendinopathy in the AT were examined using a clinical 3 Tesla (T) MRI scanner in conjunction with a dual tuned ¹H/²³Na surface coil to measure ²³Na SNR and aTSC in their ATs. ²³Na T₁ and T₂* of the AT were also measured for three controls to correct for different relaxation behavior. The results were as follows: ²³Na SNR = 11.7 ± 2.2, aTSC = 82.2 ± 13.9 mM, ²³Na T₁ = 20.4 ± 2.4 ms, ²³Na T_2s* = 1.4 ± 0.4 ms, and ²³Na T_2l* = 13.9 ± 0.8 ms for the whole AT of healthy controls with significant regional differences. These are the first reported aTSCs and ²³Na relaxation times for the AT using sodium MRI and may serve for future comparability in different studies regarding examinations of diseased ATs with sodium MRI.     

部分预应力混凝土叠合板底板力学性能试验研究

通过单因素控制变量试验,研究了预应力筋张拉控制应力与分布情况对部分预应力混凝土叠合板底板变形与抗裂性能的影响。试验结果表明,随着张拉控制应力与预应力筋分布根数的增加,叠合板底板挠度降低,抗裂性能得到极大改善。当预应力筋张拉根数为7根时,张拉控制应力从0.4fptk递增至0.7fptk,构件挠度降低了22.1%,裂缝最大宽度降低幅度可达50.0%。     

钢-混凝土组合箱型梁的滑移和剪力滞效应

该文提出了钢-混凝土组合箱型梁考虑滑移和剪力滞效应的理论模型。模型中引入了5个变量,分别包括混凝土板轴向位移、钢梁轴向位移、竖向挠度、混凝土板翘曲强度函数和钢梁翘曲强度函数,采用虚功原理得到了组合箱型梁的控制微分方程组,微分方程组可基于有限差分法求解。进而结合有限元精细模型分析手段,通过分析1座简支组合箱梁桥和1座两跨连续组合箱梁桥的滑移和剪力滞效应,验证了理论模型的准确性与适用性。最后以承受均布荷载的简支组合箱梁桥为基础,采用理论模型研究了压弯或拉弯荷载导致的梁柱效应以及预应力束布置方式对剪力滞效应的影响,并进一步分析了所提出的理论模型较之于之前研究者提出的理论模型所具备的优势。     

有黏结预应力FRP筋混凝土梁的抗弯设计

为了研究非预应力钢筋对预应力FRP筋混凝土梁抗弯性能的影响,本文定义了各种破坏模式,推导了相应的承载力计算公式,对抗弯强度的折减系数进行了分析,并通过文献中的试验数据对设计公式进行了校核,最后对体内有黏结预应力FRP筋混凝土梁的抗弯设计提出了建议.     

不同预拉力和冲击能量下BFRP筋的横向抗冲击性能

为研究玄武岩纤维增强树脂基复合材料(Basalt fiber reinforced polymer,BFRP)筋的低速冲击性能,通过落锤冲击试验测试了不同预拉力比值(2％、10％、20％和30％)和不同能量(12.76-31.90J)作用下BFRP筋的低速冲击响应,同时测试了未完全断裂试件的残余拉伸承载力.结果 表明:...