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81.
82.
An automatic image analysis method was developed to determine the shape and size of spheroidal cells from a time series of differential interference contrast (DIC) images. The program incorporates an edge detection algorithm and dynamic programming for edge linking. To assess the accuracy and working range of the method, results from DIC images of different focal planes and resolutions were compared to confocal images in which the cell membrane was fluorescently labelled. The results indicate that a 1‐µm focal drift from the in‐focus plane can lead to an overestimation of cell volume up to 14.1%, mostly due to shadowing effects of DIC microscopy. DIC images allow for accurate measurements when the focal plane lies in a zone slightly above the centre of a spherical cell. In this range the method performs with 1.9% overall volume error without taking into account the error introduced by the representation of the cell as a sphere. As a test case, the method was applied to quantify volume changes due to acute changes of osmotic stress. 相似文献
83.
R. C. Ecob 《Journal of microscopy》1985,137(3):313-337
Whereas models of the structure and periodicity of CSL or near CSL high angle grain boundaries are relatively well developed and have been to some extent verified by experiment, the nature of more general (e.g. fcc/bcc) interfaces is very imperfectly understood. One of the major differences between homo- and heterophase boundaries is the occurrence, in the latter case, of reproducible orientation relationships due to the crystallographic requirements of phase transformation (e.g. a glissile interface for martensitic growth, low energy interfaces for classical nucleation). A review is given of the relationships commonly observed to obtain between fcc and bcc crystals (as well as ordered phases based upon these structures). Within a given relationship, it is possible to use models of the available interfaces to predict their dislocation content; the success of the various approaches is considered. It is shown that the dislocation arrays identified on fcc/bcc boundaries are consistent with the occurrence of a primary structural relaxation, but that the latter does not appear to be complete. Possible reasons for this are discussed, together with the extent to which secondary structural models may be applicable. Attempts to rationalize the observed orientation relationships and morphologies are discussed, and it is pointed out that these all rely on searches for situations of minimum primary misfit, but that the way in which the latter is quantified determines the results of the analyses. Nevertheless, the implication is that the primary misfit does appear to determine the actual behaviour, though no structural or mechanistic conclusions can safely be drawn from this observation. 相似文献
84.
MCS接枝共聚物的结构和形态 总被引:4,自引:0,他引:4
将甲基丙烯酸甲酯(MMA)、苯乙烯(S)在氯化聚乙烯(CPE)存在下进行悬浮接枝共聚,获得MCS树脂。本文着重考察了MCS树脂的化学结构及胶粒形态。结果表明,确实发生了接枝共聚。CPE用量、RSH含量、引发剂浓度、反应温度、溶胀时间及聚合转化率对MCS树脂的接枝率、接枝效率均有影响。MCS树脂是接枝共聚物、MMA/S共聚物与MMA均聚物的混合物,橡胶相CPE在MCS树脂中的分散情况为“海岛结构”, 相似文献
85.
Image segmentation towards new image representation methods 总被引:1,自引:0,他引:1
Diogo Cortez Paulo Nunes Manuel Menezes de Sequeira Fernando Pereira 《Signal Processing: Image Communication》1995,6(6):485-498
Very low bit-rate video coding has recently become one of the most important areas of image communication and a large variety of applications have already been identified. Since conventional approaches are reaching a saturation point, in terms of coding efficiency, a new generation of video coding techniques, aiming at a deeper “understanding” of the image, is being studied. In this context, image analysis, particularly the identification of objects or regions in images (segmentation), is a very important step. This paper describes a segmentation algorithm based on split and merge. Images are first simplified using mathematical morphology operators, which eliminate perceptually less relevant details. The simplified image is then split according to a quad tree structure and the resulting regions are finally merged in three steps: merge, elimination of small regions and control of the number of regions. 相似文献
86.
87.
罗璇 《计算机与数字工程》2012,40(3):58-60,88
VR与GIS相结合是VR技术的一个非常重要的应用,是实现"数字地球"、"数字城市"、"数字区域"等的关键技术之一。以武汉大学信息学部教学区为研究区域,利用教学区的航空影像、数字化地图、实测数据以及区内建筑物的数字影像,在MultiGen Creator/Vega软件平台上建立该区域的虚拟现实场景,实现三维模型漫游。并将此场景用于GIS,实现了GIS属性查询功能。 相似文献
88.
为了提高肝癌手术治疗计划的可靠性,在利用腹部CT图像数据进行肝脏三维重建的基础上,实现了任意角度对虚拟肝脏进行平面切割和切割面结构的显示;通过构造可以调节大小、位置的球体模型,实现了球体切除;通过在肝脏三维模型表面上勾画不规则封闭区域,实现了任意曲面肝脏切除;并依据肝内血管结构进行肝段划分,实现了解剖性肝段切除.临床实验结果表明,文中所述的技术能够满足虚拟手术的需求,有利于医生制定安全可靠的术前计划,提高手术成功率. 相似文献
89.
当前的脉象仪大部分采集手段单一,取脉压力的调节性差,无法模拟医生的布指方式,而且只能实现脉搏图的描记及分析,无法给出脉象的三维信息。针对这一问题研制了基于柔性阵列传感器的脉象检测系统,用硅胶制作了专门的脉搏换能器;设计了压力控制系统,实现压力自动调节,完成了对最佳取脉压力下脉象的采集;搭建了信号处理电路,实现了对脉象信号的提取和预处理;以USB-7660为核心构建了A/D采集系统,实现了对多路脉象信号的显示、存储。实验表明,该系统不仅可以有效采集到受试者的七路二维脉图,而且能清晰地观测到受试者的脉道宽度,并重建出相应脉搏波的三维传导地形图。 相似文献
90.