Pro／Engineer在逆向工程曲面造型上的应用

介绍了在逆向工程曲面建模过程中,使用美国PTC公司的Pro/Engineer三维造型软件,处理由三坐标测量机所测得的大量点数据,建立符合工程要求的曲面的方法。着重阐述了由大量数据点进行不规则曲线、曲面反求的完整技术。探讨了在Pro/Engineer环境下,直接从三坐标测量机产生的数据文档生成合乎工程要求的三维曲线,进而生成三维曲面的方法。通过对实验测量的涡轮表面数据的处理,在Pro/Engineer中建立了符合工程要求的涡轮曲面,从而证实了本方法的确实可行性。     

具有细胞识别信号的组织工程支架材料的研究

组织工程是一门新兴的交叉性学科,寻找理想的具有细胞识别信号的细胞外基质替代材料是目前研究的热点之一.综述了天然聚合物材料、新兴的α-氨基酸类聚合材料等具有细胞识别信号的材料的研究和发展概况.     

开展工程伦理学研究 增强工程师责任意识

工程伦理学是20世纪后期兴起的一门交叉学科。文章首先分析了把技术开发与技术使用分开，从而回避工程师责任的做法，进而剖析了认为工程是科学的应用和工程是实现社会价值的简单工具等观点，指出工程与伦理密切相关，工程师应当增强责任意识。     

Holographic digital Fourier microscopy for selective imaging of biological tissue

We present an application of digital Fourier holography for selective imaging of scatterers with different sizes in turbid media such as biological tissues. A combination of Fourier holography and high‐resolution digital recording, digital Fourier microscopy (DFM) permits crucial flexibility in applying filtering to highlight scatterers of interest in the tissue. The high‐resolution digital hologram is a result of the collation of Fourier holographic frames to form a large‐size composite hologram. It is expected that DFM has an improved signal‐to‐noise ratio as compared to conventional direct digital imaging, e.g., phase microscopy, as applied to imaging of small‐size objects. The demonstration of the Fourier filtering capacity of DFM using a biological phantom represents the main focus of this article. © 2005 Wiley Periodicals, Inc. Int J Imaging Syst Technol, 14, 253–258, 2004; Published online inWiley InterScience (www.interscience.wiley.com). DOI 10.1002/ima.20031     

Poröse Scaffolds aus mineralisiertem Kollagen – ein biomimetisches Knochenersatzmaterial

Porous scaffolds made from mineralised collagen – a biomimetic bone graft material Using biomimetically mineralised collagen type I, a new porous bone graft material has been developed, the composition of which mimics extracellular matrix of bone tissue. The pore structure is generated by a freeze drying process, whereas the pore size can be controlled by temperature and velocity of the freezing over a wide range. The structure is stabilized by crosslinking of the collagen with the water soluble carbodiimide derivative EDC. For the seeding with bone cells, pores with diameters of about 200 μm have turned out to be optimal. These scaffolds are elastic in the wet state which allow for cell culture experiments under mechanical stimulation.     

The Experimental Study of Constructing Tissue Engineered Bone by Compounding Zinc-sintered Bovine Cancellous Bone with Marrow Stromal Cells

1　IntroductionBonetissueengineeringinvolvesconstructingtissueengineeredbonetorepairbonedefectsbyusingscaffoldswhicharecompoundedwithseededcells .Itisimportanttochooseandmanufactureidealscaffoldsinthisarea .Wehavealreadyusedbothzinc sinteredbovinecancello…     

胶原在组织工程人工皮肤中的应用

作为生物相容性良好的医用材料，胶原被广泛应用于人工皮肤。文中介绍了胶原基人工皮肤的特点、应用形式和国内外的研究情况。     

基于Pro/E NC模块的图形交互式自动编程

随着计算机技术的发展，图形交互式自动编程的应用越来越广泛，它可以直接将零件的几何图形信息转化为数控加工程序，极大地提高了数控编程的效率．文章介绍了利用Pro／engineer NC模块进行图像交互式自动编程的工作流程，并以铣削实例对编程过程中的有关参数设置进行了分析说明。     

AFM analysis of the lacunar-canalicular network in demineralized compact bone

Atomic force microscopy has been successfully used to examine a wide range of cellular and biomolecular structures and interactions. The application of atomic force microscopy in the analysis of organs and tissues, however, has been limited. In this study, we present a new method for high-resolution atomic force microscopy imaging of compact bone tissue. We performed atomic force microscopy imaging on demineralized compact bone from bovine tibia to obtain structural information about the bone matrix and the lacunar-canalicular network. Knowledge of the dimensions and distributions of the network allows quantitative analysis of the microfluidics of bone tissue. Results from our study show that (1) the canalicular distribution and dimensions are homogenous in transverse, radial and longitudinal orientations; (2) the lamellae of an osteon consist of alternating high and low bands; (3) the canaliculi follow the contour of lamellar bands and (4) globular structures cover much of the bone matrix, including canalicular walls. Our work demonstrates that atomic force microscopy studies of thin-section tissue samples can provide structural details at nanometre resolution.     

Backscattered electron imaging of the undersurface of resin-embedded cells by field-emission scanning electron microscopy

In this study backscattered electron (BSE) imaging was used to display cellular structures stained with heavy metals within an unstained resin by atomic number contrast in successively deeper layers. Balb/c 3T3 fibroblasts were cultured on either 13-mm discs of plastic Thermanox, commercially pure titanium or steel. The cells were fixed, stained and embedded in resin and the disc removed. The resin block containing the cells was sputter coated and examined in a field-emission scanning electron microscope. The technique allowed for the direct visualization of the cell undersurface and immediately overlying areas of cytoplasm through the surrounding embedding resin, with good resolution and contrast to a significant depth of about 2 μm, without the requirement for cutting sections. The fixation protocol was optimized in order to increase heavy metal staining for maximal backscattered electron production. The operation of the microscope was optimized to maximize the number of backscattered electrons produced and to minimize the spot size. BSE images were collected over a wide range of accelerating voltages (keV), from low values to high values to give ‘sections' of information from increasing depths within the sample. At 3–4 keV only structures a very short distance into the material were observed, essentially the areas of cell attachment to the removed substrate. At higher accelerating voltages information on cell morphology, including in particular stress fibres and cell nuclei, where heavy metals were intensely bound became more evident. The technique allowed stepwise ‘sectional’ information to be acquired. The technique should be useful for studies on cell morphology, cycle and adhesion with greater resolution than can be obtained with any light-microscope-based system.