Na+ and/or Cl− Toxicities Determine Salt Sensitivity in Soybean (Glycine max (L.) Merr.), Mungbean (Vigna radiata (L.) R. Wilczek), Cowpea (Vigna unguiculata (L.) Walp.), and Common Bean (Phaseolus vulgaris L.)

Grain legumes are important crops, but they are salt sensitive. This research dissected the responses of four (sub)tropical grain legumes to ionic components (Na⁺ and/or Cl⁻) of salt stress. Soybean, mungbean, cowpea, and common bean were subjected to NaCl, Na⁺ salts (without Cl⁻), Cl⁻ salts (without Na⁺), and a “high cation” negative control for 57 days. Growth, leaf gas exchange, and tissue ion concentrations were assessed at different growing stages. For soybean, NaCl and Na⁺ salts impaired seed dry mass (30% of control), more so than Cl⁻ salts (60% of control). All treatments impaired mungbean growth, with NaCl and Cl⁻ salt treatments affecting seed dry mass the most (2% of control). For cowpea, NaCl had the greatest adverse impact on seed dry mass (20% of control), while Na⁺ salts and Cl⁻ salts had similar intermediate effects (~45% of control). For common bean, NaCl had the greatest adverse effect on seed dry mass (4% of control), while Na⁺ salts and Cl⁻ salts impaired seed dry mass to a lesser extent (~45% of control). NaCl and Na⁺ salts (without Cl⁻) affected the photosynthesis (P_n) of soybean more than Cl⁻ salts (without Na⁺) (50% of control), while the reverse was true for mungbean. Na⁺ salts (without Cl⁻), Cl⁻ salts (without Na⁺), and NaCl had similar adverse effects on P_n of cowpea and common bean (~70% of control). In conclusion, salt sensitivity is predominantly determined by Na⁺ toxicity in soybean, Cl⁻ toxicity in mungbean, and both Na⁺ and Cl⁻ toxicity in cowpea and common bean.     

车联网基于信任度的RSU识别及资源分配算法

在车联网中,由于部分路侧单元（RSU）的位置比较偏远,无法得到网络管理员的及时维护,可能遭到攻击,变为不可信任的路侧单元,降低了车辆的通信质量和网络能效.为了有效地识别RSU的可靠性,提出了一种基于信任度的路侧单元识别及资源分配算法,将路侧单元识别、传输模式的选择和功率分配联合建模为网络能效的优化问题,并分成3个子优化问题,分别求解.首先,采用基于信任度的RSU识别算法,识别出可信任和不可信任的路侧单元;其次,优化车辆资源块配置和链路选择;最后,通过次梯度算法优化传输功率,最大化网络能效.理论分析和仿真结果表明,所提算法具备较低的复杂度,可以达到较高的路侧单元识别准确度,并有效地提升网络总能效.     

形位公差标注中的常见问题分析

对在形位公差标注中常出现的问题进行了分析探讨，并给出了合理性方案。     

Isolation and Functional Characterization of a Salt-Responsive Calmodulin-Like Gene MpCML40 from Semi-Mangrove Millettia pinnata

Salt stress is a major increasing threat to global agriculture. Pongamia (Millettia pinnata), a semi-mangrove, is a good model to study the molecular mechanism of plant adaptation to the saline environment. Calcium signaling pathways play critical roles in the model plants such as Arabidopsis in responding to salt stress, but little is known about their function in Pongamia. Here, we have isolated and characterized a salt-responsive MpCML40, a calmodulin-like (CML) gene from Pongamia. MpCML40 protein has 140 amino acids and is homologous with Arabidopsis AtCML40. MpCML40 contains four EF-hand motifs and a bipartite NLS (Nuclear Localization Signal) and localizes both at the plasma membrane and in the nucleus. MpCML40 was highly induced after salt treatment, especially in Pongamia roots. Heterologous expression of MpCML40 in yeast cells improved their salt tolerance. The 35S::MpCML40 transgenic Arabidopsis highly enhanced seed germination rate and root length under salt and osmotic stresses. The transgenic plants had a higher level of proline and a lower level of MDA (malondialdehyde) under normal and stress conditions, which suggested that heterologous expression of MpCML40 contributed to proline accumulation to improve salt tolerance and protect plants from the ROS (reactive oxygen species) destructive effects. Furthermore, we did not observe any measurable discrepancies in the development and growth between the transgenic plants and wild-type plants under normal growth conditions. Our results suggest that MpCML40 is an important positive regulator in response to salt stress and of potential application in producing salt-tolerant crops.     

In Vitro Characterization of Human CD24hiCD38hi Regulatory B Cells Shows CD9 Is Not a Stable Breg Cell Marker

Regulatory B (Breg) cells are endowed with immune suppressive functions. Various human and murine Breg subtypes have been reported. While interleukin (IL)-10 intracellular staining remains the most reliable way to identify Breg cells, this technique hinders further essential functional studies. Recent findings suggest that CD9 is an effective surface marker of murine IL-10 competent Breg cells. However, the stability of CD9 and its relevance as a unique marker for human Breg cells, which have been widely characterized as CD24^hiCD38^hi, have not been investigated. Here, we demonstrate that CD9 expression is sensitive to in vitro B cell stimulations. CD9 expression could either be re-expressed or downregulated in purified CD9-negative B cells and CD9-positive B cells, respectively. We found no significant differences in the Breg differentiation capacity of the CD9-negative and CD9-positive B cells. Furthermore, CD9-positive B cells co-express CD40 and CD86, suggesting their nature as B cell activation or co-stimulatory molecules, rather than regulatory ones. Therefore, we report the relatively unstable CD9 as a distinct surface molecule, indicating the need for further research for a more reliable marker to purify human Breg cells.     

基于时空特性以及需求响应的DG和EV充电站多目标优化配置

针对可再生分布式电源(DG)及电动汽车(EV)大规模接入给配电网带来的用电量增长以及电压波动问题,提出一种基于时空特性以及需求响应的DG和EV充电站多目标协调优化配置方法.通过提取城市路网的拓扑结构,监测路网流量,基于交通规划软件TransCAD进行起讫点(OD)矩阵反推,构建出行概率矩阵以描述用户的出行特性;基于蒙特卡洛方法模拟EV的时空分布特性,考虑EV、DG与常规负荷的时序特性,并基于改进K-means算法构建风-光-负荷的典型运行场景;兼顾电网侧与用户侧,以综合效益、系统负荷波动以及充电耗时成本为目标,构建DG和EV充电站的多目标联合配置模型,并采用改进粒子群优化算法进行求解.结合IEEE 33节点配电网与某城区主干道路网模型进行仿真分析,结果验证了所建模型的有效性与可行性.     

型材产生壁厚偏差的原因及解决方法

就型材生产过程中产生壁厚偏差的原因进行了分析，并就解决偏差的方法进行了探讨。