Peptidyl Virus-Like Nanovesicles as Reconfigurable “Trojan Horse” for Targeted siRNA Delivery and Synergistic Inhibition of Cancer Cells

The self-assembly of peptidyl virus-like nanovesicles (pVLNs) composed of highly ordered peptide bilayer membranes that encapsulate the small interfering RNA (siRNA) is reported. The targeting and enzyme-responsive sequences on the bilayer's surface allow the pVLNs to enter cancer cells with high efficiency and control the release of genetic drugs in response to the subcellular environment. By transforming its structure in response to the highly expressed enzyme matrix metalloproteinase 7 (MMP-7) in cancer cells, it helps the siRNA escape from the lysosomes, resulting in a final silencing efficiency of 92%. Moreover, the pVLNs can serve as reconfigurable “Trojan horse” by transforming into membranes triggered by the MMP-7 and disrupting the cytoplasmic structure, thereby achieving synergistic anticancer effects and 96% cancer cell mortality with little damage to normal cells. The pVLNs benefit from their biocompatibility, targeting, and enzyme responsiveness, making them a promising platform for gene therapy and anticancer therapy.     

Design and Configuration Rationales for Digital Video Storage and Delivery Systems

Recent advances in computing technology have brought multimedia information processing to prominence. The ability to digitize, store, retrieve, process, and transport analog information in digital form has changed the dimensions of information handling. Several architectural and network configurations have been proposed for efficient and reliable digital video delivery systems. However, these proposals succeed only in addressing subsets of the whole problem. In this paper, we discuss the characteristics of video services. These include Cable Television, Pay-Per-View, and Video Repository Centers. We also discuss requirements for Video On Demand services. With respect to these video services, we analyze two important video properties: image quality and response time. We discuss and present configurations of a Digital Video Delivery System (DVDS) from three general system components - servers, clients, and connectivities. Pertinent issues in developing each component are also analyzed. We also present an architecture of a DVDS that can support the various functionalities that exist in the various video services. Lastly, we discuss data allocation strategies which impact performance of interactive video on demand (IVOD). We present preliminary results from a study using a limited form of mirroring to support high performance IVOD.     

可靠实现煤矿双机热备系统的方法

在分析商用双机热备系统在煤矿应用的局限性基础上,提出了一种适用于中小煤矿的双机热备系统纯软件实现方法。该方法采用单/双工混合运行模式保证双机实时性能和数据一致性,采用心跳线连接和对等网络消息传递机制实现运行故障监测与传递功能,采用数据自动同步技术减少人工干预和提高系统可维护性,并采用虚拟IP地址技术实现应用层透明访问和无缝切换功能。实验与应用案例证明该方法符合相关行业安全标准并具备现实可行性。     

Coordination of split deliveries in one-warehouse multi-retailer distribution systems

In this paper, we consider a distribution system where a warehouse is responsible for replenishing the inventories at multiple retailers by a fleet of vehicles of limited capacity. If a distribution policy of the system involves split deliveries, that is, the inventory of at least one retailer is replenished by using multiple vehicle routes, the coordination of the deliveries can further reduce the inventory cost of the retailer. We consider the coordination where two split deliveries are realized by direct shipping and multiple-stop shipping, respectively. To the best of our knowledge, this kind of coordination was never studied in the literature but can find its application in inventory routing problems. This paper proposes and analyses a class of coordination policies for the split deliveries which can reduce the inventory costs of the retailers without increasing transportation costs. A non-linear programming model is established for formulating the class of polices. Because the optimal coordination policy corresponding to an optimal solution of the model may be hard to find and/or implement, two simple but effective coordination policies are proposed. The inventory cost savings realized by the two policies are evaluated analytically and algorithmically. Our theoretical analysis and computational experiments show that both policies are effective. Under certain conditions, they can save 50% of the inventory costs at the retailers without increasing transportation costs.     

移动计算环境中的自适应混合广播

提出一种自适应混合广播算法。在周期广播数据分配时采用多信道非均匀分配,使高访问率的数据获得高广播频率。在确定周期广播数据和联机请求数据个数时考虑联机请求信道响应时间和访问率之间的关系,从而在少量比较次数后获得数据最佳分割点。实验结果表明,该算法可以根据系统负载和用户访问模式的变化动态调节信道和数据的分配,性能优于纯广播和纯基于请求的广播,访问时间少于现有的混合数据广播方式。     

发布／订阅范型中信息可靠投递的研究

以发布／订阅范型在时间维度上解耦导致的不确定性为出发点,采用时序分析方法,研究范型中信息投递的可靠性保障。在定义投递信息可用性的基础上,给出保证信息向订阅者可靠投递系统应满足的基本计算条件,探讨底层通信设施对可靠性的影响。针对发布／订阅范型中信息可靠投递的问题,提出必要的计算条件。分析结果表明,信息的可靠投递与订阅活跃期、订阅延迟、发布传播延迟密切相关。     

空空导弹投放信号中断故障容错设计与PHM实现

载机送出投放信号启动空空导弹弹射过程,当该过程尚未结束而投放信号中断或瞬断时,则会产生机-弹分离安全性问题.为了防止该问题的发生,在弹射控制电路的基础上,采用高能复合钽电解电容储存电能的方法实现投放信号中断故障容错.在电容充电过程中,通过在线测试充电电压得到实际电容充电曲线,比较该曲线和标准曲线的均方误差进行故障预测和健康管理.试验表明了故障容错设计和故障预测的可行性和有效性.     

基函数自适应选择的预测函数控制方法在混凝投加中的应用

传统预测函数控制算法的基函数是固定的,在控制环境不稳定的情况下控制品质不理想,本文提出一种通过对基函数的自适应选择来改善控制品质。本文根据过程输出值与期望值的误差来选择基函数,将全体误差分为几个区域,每个区域分别对应一个基函数,通过计算误差落选区域来选择对应的基函数。针对混凝投药过程的时滞原因,本文利用软测量方法建立一个混凝投药模型,应用该模型预测过程的输出。Matlab仿真表明此算法具有更好的平稳性和快速响应能力,比传统预测函数控制算法具有更好的控制品质。     

基于投递概率预测的DTN高效路由

在延迟容忍网络中,基于配额的多拷贝路由通过对消息拷贝数量的限制来控制开销,如何在控制开销的前提下尽可能提高消息投递成功率是其需要解决的关键问题.文中首先分析了提高消息投递成功率的配额分配策略,然后据此提出一种基于投递概率预测的配额路由算法.该路由算法根据节点的本地信息预测到其它节点的投递概率,在复制消息时,根据节点间投...